Journal: BMC Complementary Medicine and Therapies

Article Title: Biochemical and cellular effects of squalene on human gingival fibroblasts in an in vitro wound healing model

doi: 10.1186/s12906-026-05298-2

Figure Lengend Snippet: Inverted microscopy images of HGF cells in the control, SQ1, and SQ2 groups at baseline and 24, 48, and 72 hours following the scratch assay

Article Snippet: An HGF cell line (HGF-1, ATCC, CRL-2014, Manassas, VA, USA) was used in this study.

Techniques: Inverted Microscopy, Control, Wound Healing Assay

Journal: The Journal of investigative dermatology

Article Title: Downregulation of OPN3 Gene Induces Ferroptosis, Apoptosis, and Pyroptosis of Human Dermal Fibroblasts In Vitro.

doi: 10.1016/j.jid.2024.03.018

Figure Lengend Snippet: Figure 1. OPN3 knockdown promotes cell ferroptosis. (a) Protein levels of OPN3 and ferroptosis-related proteins (TFRC, FTH, FTL, FPN, ACSL4, GPX4, and NCOA4) were compared between the LV-control-RNAi group and the LV-OPN3-RNAi group using western blotting. (b) CCK8 assays were performed on LV-OPN3-RNAi and LV-control-RNAi cells treated with DMSO or 4 mM Lip-1. *P < .05, **P < .01, and ***P < .001 versus the LV-OPN3-RNAi cells treated with the ferroptosis inhibitor Lip-1. (c) Relative iron levels were assessed using FerroOrange fluorescent probes in NHDFs. (d) ROS and (e) lipid ROS levels in NHDFs were detected through flow cytometry analysis (control: no probe). (f) Transmission electron microscopy images of the LV-control-RNAi, LV-OPN3-RNAi, and RSL3 (0.006 mM, 24 hours) groups. Red arrows indicate the morphological features of mitochondria. Bars ¼ 5 mm, 2 mm, and 500 nm (from left to right). Error bars represent SDs. *P < .05, **P < .01, and ***P < .001. Lip-1 is a ferroptosis inhibitor. ACSL4, acyl-CoA synthetase long-chain family member 4; CCK8, Cell Counting Kit 8; FTH, ferritin heavy chain; FTL, ferritin light chain; FPN, ferroportin; GPX4, glutathione peroxidase 4; Lip-1, liproxstatin-1; NHDF, normal human dermal fibroblast; RNAi, RNA interference; TFRC, transferrin receptor.

Article Snippet: Normal human dermal fibroblasts (NHDFs) were derived from discarded prepuce from pediatric patients aged 3e12 years (Affiliated Hospital of Guizhou Medical University, Guiyang, China) in an experiment with patient consent, and the research protocol was approved by the ethics committee of the Affiliated Hospital of Guizhou Medical University.

Techniques: Knockdown, Control, Western Blot, Cytometry, Transmission Assay, Electron Microscopy, Cell Counting

Journal: The Journal of investigative dermatology

Article Title: Downregulation of OPN3 Gene Induces Ferroptosis, Apoptosis, and Pyroptosis of Human Dermal Fibroblasts In Vitro.

doi: 10.1016/j.jid.2024.03.018

Figure Lengend Snippet: Figure 2. OPN3 knockdown promotes cell ferroptosis, apoptosis, and pyroptosis in NHDFs. (a) Knockdown of OPN3 gene induced apoptosis by flow cytometry analysis; PI/annexin staining was performed at 24 hours. (b) Western blotting analyses of BCL-2, BAX, and BAX/BCL-2 protein levels between LV-control-RNAi group and LV-OPN3-RNAi group. (c) Transmission electron microscopy images of LV-control-RNAi group and LV-OPN3-RNAi group. Five low magnification fields were taken at random to count different cell death types. The red, black, blue, and green arrows indicated ferroptosis, apoptosis, pyroptosis, and apoptotic bodies, respectively. The bar in the first row and the final 2 panels is 10 mm, and the rest is 2 mm. (d) Protein levels of OPN3 and pyroptosis related proteins (Gasdermin D, cleaved Gasdermin D, caspase 1, Cleaved caspase 1, and IL-1b) were detected between LV-control-RNAi group and LV-OPN3- RNAi group by western blotting. PI, propidium iodide; RNAi, RNA interference.

Article Snippet: Normal human dermal fibroblasts (NHDFs) were derived from discarded prepuce from pediatric patients aged 3e12 years (Affiliated Hospital of Guizhou Medical University, Guiyang, China) in an experiment with patient consent, and the research protocol was approved by the ethics committee of the Affiliated Hospital of Guizhou Medical University.

Techniques: Knockdown, Cytometry, Staining, Western Blot, Control, Transmission Assay, Electron Microscopy