Journal: medRxiv

Article Title: CRABP2 Upregulation in Perichondral Stem Cells is Associated with Microtia

doi: 10.64898/2025.12.18.25342452

Figure Lengend Snippet: (A) Boxplot shows the RA responsiveness score measured by three different gene sets. (B) Dot plot shows the top 10 active regulons predicted by pyscenic in the COL1 + HES1 + perichondral stem cells from normal and microtia samples respectively. (C) Gene ontology enrichment results of genes up-regulated in COL1 + HES1 + perichondral stem cells from normal samples (left) and in microtia samples (right). (D) Expression level of RA associated genes in COL1 + HES1 + perichondral stem cells from normal samples (left) and in microtia samples (right). ***P<0.001, Student’s t test.

Article Snippet: The following primary antibodies and dilutions were used: COL-1 (1:150, 67288-1-Ig, Proteintech, China), CoraLite 594-conjugated CRABP2 (1:75, CL594-10225, Proteintech, China), and HES1 (1:200, 11988, CST, United States).

Techniques: Expressing

Journal: medRxiv

Article Title: CRABP2 Upregulation in Perichondral Stem Cells is Associated with Microtia

doi: 10.64898/2025.12.18.25342452

Figure Lengend Snippet: (A) Representative immunofluorescence staining images of auricular cartilage slices from microtia and normal samples. Scale bar for large images: 150 µm. Scale bar for insets: 50 µm. (B) Line intensity profiles of DAPI, anti-COL1, anti-CRABP2 and anti-HES1 immunostainings. (C) Boxplot shows the quantification result of CRABP2 + COL1 + HES1 + proportions among COL1 + HES1 + perichondral stem cells in normal and microtia samples. *P<0.05, Student’s t test.

Article Snippet: The following primary antibodies and dilutions were used: COL-1 (1:150, 67288-1-Ig, Proteintech, China), CoraLite 594-conjugated CRABP2 (1:75, CL594-10225, Proteintech, China), and HES1 (1:200, 11988, CST, United States).

Techniques: Immunofluorescence, Staining

Journal: Aging and Disease

Article Title: Mash1-dependent Notch Signaling Pathway Regulates GABAergic Neuron-Like Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells

doi: 10.14336/AD.2016.1018

Figure Lengend Snippet: Primer sequences for Notch signaling and GABAergic neuron marker

Article Snippet: For RBPJ or Hes1 silencing, BMSCs which reached approximately 50% confluence in a 12-well plate were replaced with Polybrene (Santa Cruz Biotechnology, Inc., sc-134220, CA, USA) media mixture, followed by infection with RBPJ (Santa Cruz Biotechnology, Inc., sc-270318-v, CA, USA) or Hes1 (Santa Cruz Biotechnology, Inc., sc-270146-v, CA, USA) shRNA lentiviral particles (these primers were provided by the company) overnight (37°C, 5% CO 2 ).

Techniques: Sequencing

Journal: Aging and Disease

Article Title: Mash1-dependent Notch Signaling Pathway Regulates GABAergic Neuron-Like Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells

doi: 10.14336/AD.2016.1018

Figure Lengend Snippet: A ) Protein bands of Notch signaling (RBPJ, Hes1 and Mash1) visualized through western blotting of BMSCs that were treated by DAPT (DAPT+BMSCs), or engineered by Hes1 shRNA (Hes1-BMSCs), pGC-FU-Mash1 plasmid (Mash1+BMSCs), RBPJ shRNA (RBPJ-BMSCs) and copGFP Control (Lv-con-BMSCs). B ) The protein level based on the ratio of Gauss Model Trace exhibited the Notch signaling (RBPJ, Hes1 and Mash1) changes in the DAPT+ BMSCs. C ) The protein level based on the ratio of Gauss Model Trace exhibited genetically engineered BMSCs. Error bars in bar graphs display standard deviation (SD). * P = 0.032, < 0.05, ** P = 0.0006, < 0.01.

Article Snippet: For RBPJ or Hes1 silencing, BMSCs which reached approximately 50% confluence in a 12-well plate were replaced with Polybrene (Santa Cruz Biotechnology, Inc., sc-134220, CA, USA) media mixture, followed by infection with RBPJ (Santa Cruz Biotechnology, Inc., sc-270318-v, CA, USA) or Hes1 (Santa Cruz Biotechnology, Inc., sc-270146-v, CA, USA) shRNA lentiviral particles (these primers were provided by the company) overnight (37°C, 5% CO 2 ).

Techniques: Western Blot, shRNA, Plasmid Preparation, Control, Standard Deviation

Journal: World Journal of Gastroenterology

Article Title: Downregulation of Hes1 expression in experimental biliary atresia and its effects on bile duct structure

doi: 10.3748/wjg.v24.i29.3260

Figure Lengend Snippet: Primers used in the experiments

Article Snippet: To study the interruption of gene expression, such as interruption of the Notch signaling pathway target molecule Hes1, in the IBEC 3D cell culture, Hes1 siRNA RNA and control siRNA purchased from Santa Cruz Biotechnology, Inc., were used in the cell culture and transfected into cells using Lipofectamine ® RNAiMAX Reagent (Life Technologies, 13778) following the manufacturer’s instructions.

Techniques:

Journal: World Journal of Gastroenterology

Article Title: Downregulation of Hes1 expression in experimental biliary atresia and its effects on bile duct structure

doi: 10.3748/wjg.v24.i29.3260

Figure Lengend Snippet: Expression levels of Notch signaling components in the liver of rhesus rotavirus-inoculated mice. The mRNA levels of Notch signaling components, including Jag1, Dll1, Notch1, Notch2 and Hes1, were detected using quantitative real-time PCR with appropriate primers at day 7 and day 14 after RRV inoculation. A: The relative expression levels compared with the normal control are shown for day 7 ( a P < 0.05; b P < 0.01; n = 6 in the control group and n = 10 in the RRV group); B: day 14 ( c P < 0.001); C: The levels of the Hes1 protein and the expression of the transcriptional coregulator RBP-Jκ were detected by Western blot ( n = 2 or 3 in each group; the proteins were mixed and loaded).

Article Snippet: To study the interruption of gene expression, such as interruption of the Notch signaling pathway target molecule Hes1, in the IBEC 3D cell culture, Hes1 siRNA RNA and control siRNA purchased from Santa Cruz Biotechnology, Inc., were used in the cell culture and transfected into cells using Lipofectamine ® RNAiMAX Reagent (Life Technologies, 13778) following the manufacturer’s instructions.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Control, Western Blot

Journal: World Journal of Gastroenterology

Article Title: Downregulation of Hes1 expression in experimental biliary atresia and its effects on bile duct structure

doi: 10.3748/wjg.v24.i29.3260

Figure Lengend Snippet: Hes1 siRNA in 3D biliary epithelial cell culture. Biliary epithelial cells (BECs) were cultured in either normal growth medium (N. medium) or in matrix + collagen mixed gel (detailed components are given in the Materials and Methods section). The cells were cultured without a transfection reagent (Cont.), with Lipofectamine (Lipo) only, with irrelevant control siRNA (Cont. siRNA) or with Hes1 siRNA for seven days, then fixed and stained with anti-CK19, γ-GT and AFP antibodies, and finally counter-stained with Alexa Fluor ® 488. Photographs of the cells were taken with a Leica DMi8 inverted fluorescence microscope, and the groups were then compared. The experiment was duplicated, and one set of representative results is shown here.

Article Snippet: To study the interruption of gene expression, such as interruption of the Notch signaling pathway target molecule Hes1, in the IBEC 3D cell culture, Hes1 siRNA RNA and control siRNA purchased from Santa Cruz Biotechnology, Inc., were used in the cell culture and transfected into cells using Lipofectamine ® RNAiMAX Reagent (Life Technologies, 13778) following the manufacturer’s instructions.

Techniques: Cell Culture, Transfection, Control, Staining, Fluorescence, Microscopy

Journal: International journal of cancer

Article Title: Modification of α2,6-sialylation mediates the invasiveness and tumorigenicity of non-small cell lung cancer cells in vitro and in vivo via Notch1/Hes1/MMPs pathway.

doi: 10.1002/ijc.31737

Figure Lengend Snippet: Figure 4: Depletion of ST6Gal-I in A549 and H1299 cells inhibits the Notch1/Hes1/MMPs signaling pathway.

Article Snippet: Membranes were blocked for non-specific binding and incubated with primary antibodies directed against ST6Gal-I (polyclonal antibody, 14355-1-AP), DLL-1 (polyclonal antibody, 20230-1-AP), Notch1 (polyclonal antibody, 20687-1-AP), Hey1 (polyclonal antibody, 19929-1-AP), MMP-2 (polyclonal antibody, 10373-2-AP), MMP-7 (polyclonal antibody, 10374-2-AP), MMP-9 (polyclonal antibody, 10375-2-AP) or VEGF (polyclonal antibody, 19003-1-AP) (1:500, Proteintech), Hes1 (polyclonal antibody, EAP2709) or Jagged-1 (polyclonal antibody, ENK5401) (1:500, Elabscience), and GAPDH (polyclonal antibody, AP0063) (1:6000, Bioworld).

Techniques:

Journal: International journal of cancer

Article Title: Modification of α2,6-sialylation mediates the invasiveness and tumorigenicity of non-small cell lung cancer cells in vitro and in vivo via Notch1/Hes1/MMPs pathway.

doi: 10.1002/ijc.31737

Figure Lengend Snippet: Figure 6: ST6Gal-I knockdown inhibits the lung tumor formation in nude mice through Notch1/Hes1/MMPs

Article Snippet: Membranes were blocked for non-specific binding and incubated with primary antibodies directed against ST6Gal-I (polyclonal antibody, 14355-1-AP), DLL-1 (polyclonal antibody, 20230-1-AP), Notch1 (polyclonal antibody, 20687-1-AP), Hey1 (polyclonal antibody, 19929-1-AP), MMP-2 (polyclonal antibody, 10373-2-AP), MMP-7 (polyclonal antibody, 10374-2-AP), MMP-9 (polyclonal antibody, 10375-2-AP) or VEGF (polyclonal antibody, 19003-1-AP) (1:500, Proteintech), Hes1 (polyclonal antibody, EAP2709) or Jagged-1 (polyclonal antibody, ENK5401) (1:500, Elabscience), and GAPDH (polyclonal antibody, AP0063) (1:6000, Bioworld).

Techniques: Knockdown

Journal: Cancer cell

Article Title: Therapeutic effect of ?-secretase inhibition in Kras G12V -driven non-small cell lung carcinoma through derepression of DUSP1 phosphatase and inhibition of ERK

doi: 10.1016/j.ccr.2012.06.014

Figure Lengend Snippet: (A) Luciferase activity of H358 cells transfected with a DUSP1-luc reporter together with a plasmid expressing HES1 or its corresponding empty control. Cells were treated for 48 h with DAPT or its vehicle in the presence of complete medium.

Article Snippet: Cells were treated for 48 h with a MEK inhibitor (PD0325901) or its vehicle in the presence of complete medium. (C) ChIP analysis of the DUSP1 promoter using 2 different HES1 antibodies (Ab1, 4H1 Novus Biologicals; Ab2, H140 Santa Cruz) in H358 cells.

Techniques: Luciferase, Activity Assay, Transfection, Plasmid Preparation, Expressing, Control

Journal: Cancer cell

Article Title: Therapeutic effect of ?-secretase inhibition in Kras G12V -driven non-small cell lung carcinoma through derepression of DUSP1 phosphatase and inhibition of ERK

doi: 10.1016/j.ccr.2012.06.014

Figure Lengend Snippet: (A) Kaplan-Meier curve indicating that tumors displaying high expression of nuclear HES1 show shorter overall survival (p=0.045). High nuclear HES1 expression corresponds to tumors with at least 40% of their neoplastic cells positive for HES1.

Article Snippet: Cells were treated for 48 h with a MEK inhibitor (PD0325901) or its vehicle in the presence of complete medium. (C) ChIP analysis of the DUSP1 promoter using 2 different HES1 antibodies (Ab1, 4H1 Novus Biologicals; Ab2, H140 Santa Cruz) in H358 cells.

Techniques: Expressing