Journal: Cell Reports Medicine

Article Title: Receptor transfer between immune cells by autoantibody-enhanced, CD32-driven trogocytosis is hijacked by HIV-1 to infect resting CD4 T cells

doi: 10.1016/j.xcrm.2024.101483

Figure Lengend Snippet:

Article Snippet: Heparinase III , New England Biolabs , Cat#P0737S.

Techniques: Purification, Virus, Recombinant, Staining, Western Blot, Cell Isolation, Isolation, Marker, BIA-KA, Software, Membrane, Cell Culture

Journal: Biomolecules

Article Title: Glycan Epitopes on 201B7 Human-Induced Pluripotent Stem Cells Using R-10G and R-17F Marker Antibodies

doi: 10.3390/biom11040508

Figure Lengend Snippet: Characterization of the purified R-10G-binding protein by glycosidase digestion and Western blotting. Purified R-10G-binding protein (0.5–4 ng) incubated with (+) or without (−) glycosidases was subjected to SDS-PAGE on a 4–15% gradient polyacrylamide gel under non-reducing conditions. The exception was PNGase F digestion, which requires preheating under reducing conditions. Western blotting using R-10G was subsequently conducted. ( A ) Purified R-10G-binding protein from 201B7 cells was digested with PNGase F (lane 1), chondroitinase ABC (lane 2), a heparinase mixture (lane 3), α1-3/4 fucosidase (lane 4), α1-2 fucosidase (lane 5), neuraminidase (lane 6), and keratanase (lane 7), and the digests were analyzed by Western blotting using R-10G. ( B ) Purified R-10G-binding protein from 201B7 (lanes 1, 3) and Tic (lanes 2, 4) cells incubated with (+) or without (−) endo-β-galactosidase (lanes 1, 2) or keratanase II (lanes 3, 4) was analyzed by Western blotting using R-10G.

Article Snippet: Chondroitinase ABC ( Proteus vulgaris ), heparinase mixture (heparinase, heparitinase I, and heparitinase II), keratanase ( Pseudomonas sp.), keratanase II ( Bacillus sp.), and endo-β-galactosidase ( Escherichia freundii ) were obtained from Seikagaku Biobusiness (Tokyo, Japan).

Techniques: Purification, Binding Assay, Western Blot, Incubation, SDS Page