Journal: Discover Oncology

Article Title: Multi-omics integration of TPX2 in prognostic prediction in resectable hepatocellular carcinoma

doi: 10.1007/s12672-025-02828-1

Figure Lengend Snippet: ( A ) TPX2 expression in normal hepatocytes and hepatocellular carcinoma cell lines. B TPX2 knockdown and ( C ) overexpression validation in Hep3B and Huh7 cells by qPCR and Western blot. D CCK8 assay of cell proliferation after TPX2 overexpression and knockdown in Hep3B and Huh7. E EdU assay showing cell proliferation in TPX2 knockdown and ( F ) overexpression groups. TPX2 Targeting protein for Xenopus kinesin-like protein 2; qPCR quantitative Polymerase Chain Reaction CCK8: Cell Counting Kit-8; EdU 5-Ethynyl-2'-deoxyuridine;

Article Snippet: HCC cell lines Hep3B (Procell, China), HuH7 (Procell, China), HCCLM3 (Procell, China), HepG2(Shanghai Cell Bank), SK-hep-1 (Procell, China), MHCC97H(Shanghai Cell Bank) and the immortalized human hepatocyte cell line MIHA (Syngene, China). cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) and Minimum Essential Medium (MEM) containing 10% FBS and 1%penicillin and streptomycin, respectively.

Techniques: Expressing, Knockdown, Over Expression, Biomarker Discovery, Western Blot, CCK-8 Assay, EdU Assay, Real-time Polymerase Chain Reaction, Cell Counting

Journal: Discover Oncology

Article Title: Multi-omics integration of TPX2 in prognostic prediction in resectable hepatocellular carcinoma

doi: 10.1007/s12672-025-02828-1

Figure Lengend Snippet: ( A )Wound-healing assay after TPX2 knockdown and B overexpression in Hep3B and Huh7 cells. C Transwell invasion assay in Hep3B and Huh7 cells after TPX2 knockdown and(D) overexpression. E The expression levels of DNA repair related proteins BRCA1 and RAD51 by Western blot after TPX2 knock-down in Hep3B and Huh7 cells. F the changes of cell cycle after TPX2 knock-down in Hep3B and Huh7 cells. TPX2 Targeting protein for Xenopus kinesin-like protein 2; BRCA1 Breast Cancer Type 1 Susceptibility Protein; RAD51 RAD51 Recombinase

Article Snippet: HCC cell lines Hep3B (Procell, China), HuH7 (Procell, China), HCCLM3 (Procell, China), HepG2(Shanghai Cell Bank), SK-hep-1 (Procell, China), MHCC97H(Shanghai Cell Bank) and the immortalized human hepatocyte cell line MIHA (Syngene, China). cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) and Minimum Essential Medium (MEM) containing 10% FBS and 1%penicillin and streptomycin, respectively.

Techniques: Wound Healing Assay, Knockdown, Over Expression, Transwell Invasion Assay, Expressing, Western Blot

Journal: Aging Cell

Article Title: Liver osteopontin is required to prevent the progression of age‐related nonalcoholic fatty liver disease

doi: 10.1111/acel.13183

Figure Lengend Snippet: Liver OPN is p53 regulated. (a) Protein levels of p53 were evaluated by immunohistochemistry in liver sections of 3‐, 10‐, and 20‐month‐old (m) wild‐type (WT) mice ( n = 3–5). (b) OPN protein levels from liver homogenates were measured in WT and p53‐KO male and female mice fed a chow diet (CD) and a high‐fat diet (HFD) by immunoblotting using glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) as loading control ( n = 4–6). (c) Liver p53 and OPN protein levels were evaluated in WT mice fed a HFD injected with p53‐dominant positive adenovirus (adp53) and GFP (adGFP), using transferrin or GAPDH as a loading control ( n = 4–6). (d) Protein levels of p53 and OPN in siCtrl and sip53 HepG2 cells were measured by immunoblotting using GAPDH as loading control. Extracellular OPN was measured using an ELISA ( n = 4–5). (e) OPN protein levels from HepG2 cells silenced for p53 and Hep3B cells treated with palbociclib (Palbo) were measured by immunoblotting using GAPDH as loading control. OPN media levels from Hep3B cells treated either with vehicle (Veh) or with palbociclib (Palbo) were measured by ELISA ( n = 4–8). Values are means ± SEM . Significant differences are denoted by * p < 0.05, ** p < 0.01, and *** p < 0.001 (Student's t test)

Article Snippet: Data obtained from the Cell Line Encyclopedia (© 2019 The Broad Institute of MIT & Harvard) showed that the cell line with the least p53 expression, the Hep3B, also had the lowest OPN expression.

Techniques: Immunohistochemistry, Western Blot, Control, Injection, Enzyme-linked Immunosorbent Assay

Journal: Archives of Medical Science : AMS

Article Title: MicroRNA-490-3p suppresses hepatocellular carcinoma cell proliferation and migration by targeting the aurora kinase A gene ( AURKA )

doi: 10.5114/aoms.2019.91351

Figure Lengend Snippet: miR-490-3p suppresses expression of aurora kinase A gene ( AURKA ) in hepatocellular carcinoma (HCC) cells. A – qRT-PCR showed that miR-390-3p mimic transfection could significantly increase expression of miR-390-3p in HepG2 cells. B – qRT-PCR showed that miR-390-3p mimic transfection could significantly increase expression of miR- 390-3p in HepG2 cells. C – qRT-PCR showed that AURKA mRNA expression of miR-490-3p mimics and AURKA siRNA group was significantly lower, while AURKA mRNA expression of AURKA cDNA group was higher, and miR-490-3p + AURKA cDNA had no influence on AURKA mRNA expression in HepG2 cells. D – qRT-PCR showed that AURKA mRNA expression of miR-490-3p mimics and AURKA siRNA group was significantly lower, while AURKA mRNA expression of AURKA cDNA group was higher, and miR-490-3p + AURKA cDNA had no influence on the AURKA mRNA expression in Hep3B cells. E – Western blot showed that AURKA protein expression of miR-490-3p mimics and AURKA siRNA group was significantly lower, while AURKA protein expression of AURKA cDNA group was higher, and miR-490-3p + AURKA cDNA had no influence on AURKA protein expression in HepG2 cells. F – Western blot showed that AURKA protein expression of miR-490-3p mimics and AURKA siRNA group was significantly lower, while AURKA protein expression of AURKA cDNA group was higher, and miR-490-3p + AURKA cDNA had no influence on AURKA protein expression in Hep3B cells *p < 0.05, **p < 0.01, compared with negative control (NC) group.

Article Snippet: Human embryonic kidney cell line HEK-293T, human HCC cell lines Huh-7, HepG2, Hep3B and SK-HEP1 and normal liver cell line HL-7702 were purchased from the Japanese Collection of Research Bioresources (Tokyo, Japan).

Techniques: Expressing, Quantitative RT-PCR, Transfection, Western Blot, Negative Control

Journal: Archives of Medical Science : AMS

Article Title: MicroRNA-490-3p suppresses hepatocellular carcinoma cell proliferation and migration by targeting the aurora kinase A gene ( AURKA )

doi: 10.5114/aoms.2019.91351

Figure Lengend Snippet: Impact of miR-490-3p and aurora kinase A gene ( AURKA ) on proliferation of hepatocellular carcinoma (HCC) cells. A – Colony formation images of HepG2 and Hep3B cells showed that colony number of miR-490-3p mimics and AURKA siRNA group was significantly smaller, while colony number area of AURKA cDNA group was larger, and miR-490-3p + AURKA cDNA had no influence on proliferation of the cells. B – Statistical graph of colony formation of HepG2 cells. C – Statistical graph of colony formation of Hep3B cells. D – MTT assay showed that the cell viability of miR-490-3p mimics and AURKA siRNA group was significantly lower, while the cell viability area of AURKA cDNA group was higher, and miR-490-3p + AURKA cDNA had no influence on HepG2 cell viability. E – MTT assay showed that cell viability of miR-490-3p mimics and AURKA siRNA group was significantly lower, while cell viability area of AURKA cDNA group was higher, and miR-490-3p + AURKA cDNA had no influence on Hep3B cell viability *p < 0.05, **p < 0.01, compared with negative control (NC) group.

Article Snippet: Human embryonic kidney cell line HEK-293T, human HCC cell lines Huh-7, HepG2, Hep3B and SK-HEP1 and normal liver cell line HL-7702 were purchased from the Japanese Collection of Research Bioresources (Tokyo, Japan).

Techniques: MTT Assay, Negative Control

Journal: Archives of Medical Science : AMS

Article Title: MicroRNA-490-3p suppresses hepatocellular carcinoma cell proliferation and migration by targeting the aurora kinase A gene ( AURKA )

doi: 10.5114/aoms.2019.91351

Figure Lengend Snippet: Impact of miR-490-3p and aurora kinase A gene ( AURKA ) on migration of hepatocellular carcinoma cells. A – Wound healing images of HepG2 and Hep3B cells showed that closed wound area of miR-490-3p mimics and AURKA siRNA group was significantly smaller, while closed wound area of AURKA cDNA group was larger, and miR-490-3p + AURKA cDNA had no influence on migration of the cells. B – Statistical graph of closed wound area of HepG2 cells. C – Statistical graph of closed wound area of Hep3B cells *p < 0.05, **p < 0.01, compared with negative control (NC) group.

Article Snippet: Human embryonic kidney cell line HEK-293T, human HCC cell lines Huh-7, HepG2, Hep3B and SK-HEP1 and normal liver cell line HL-7702 were purchased from the Japanese Collection of Research Bioresources (Tokyo, Japan).

Techniques: Migration, Negative Control

Journal: Archives of Medical Science : AMS

Article Title: MicroRNA-490-3p suppresses hepatocellular carcinoma cell proliferation and migration by targeting the aurora kinase A gene ( AURKA )

doi: 10.5114/aoms.2019.91351

Figure Lengend Snippet: Impact of miR-490-3p and aurora kinase A gene ( AURKA ) on invasion of hepatocellular carcinoma cells. A – Transwell images of HepG2 and Hep3B cells showed that invasive cell number of miR-490-3p mimics and AURKA siRNA group was significantly decreased, while invasive cell number of AURKA cDNA group was increased, and miR-490-3p + AURKA cDNA had no influence on invasion of the cells. B – Statistical graph of invasive cell number of HepG2 cells. C – Statistical graph of invasive cell number of Hep3B cells *p < 0.05, **p < 0.01, compared with negative control (NC) group.

Article Snippet: Human embryonic kidney cell line HEK-293T, human HCC cell lines Huh-7, HepG2, Hep3B and SK-HEP1 and normal liver cell line HL-7702 were purchased from the Japanese Collection of Research Bioresources (Tokyo, Japan).

Techniques: Negative Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Antioxidant and Anti-Cancer Potentials of Rheum emodi Rhizome Extracts

doi: 10.1093/ecam/neq048

Figure Lengend Snippet: IC 50 values of aqueous and methanolic extracts of R. emodi in MDA-MB-435S and Hep3B cell lines as determined in XTT assay.

Article Snippet: MDA-MB-435S (human breast carcinoma) and Hep3B (human liver carcinoma) cell lines were obtained from National Centre for Cell Science (Pune, India).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: The Beneficial Effects of Morusin, an Isoprene Flavonoid Isolated from the Root Bark of Morus

doi: 10.3390/ijms21186541

Figure Lengend Snippet: The Effects of Morusin on Subtypes of Cancer.

Article Snippet: Colon Liver Breast , HT-29 Hep3B MCF-7 , Apoptosis induction & suppression of NF-κB activity inhibits the phosphorylation of IKK-α, IKK-β and IκB-α and suppresses NF-κB nuclear localization and its DNA binding causes activation of caspase-8, change of mitochondrial membrane potential, release of Cytochrome c and Smac/DIABLO and activation of caspase-9 and caspase-3 , in vitro , [ ] .

Techniques: Inhibition, Expressing, In Vitro, Phospho-proteomics, In Vivo, Concentration Assay, Migration, Activation Assay, Marker, Activity Assay, Blocking Assay, Transformation Assay, Protein Binding, Membrane, Protein-Protein interactions, Binding Assay