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Image Search Results
Journal: Oncogene
Article Title: Downregulation of human polo-like kinase activity by antisense oligonucleotides induces growth inhibition in cancer cells.
doi: 10.1038/sj.onc.1205412
Figure Lengend Snippet: Figure 1 Reduction of PLK1 mRNA in vitro. (a) Relative positioning of the predicted hybridization sites of the 26 tested PLK1 ASOs. Sequences of all ASOs are available upon request. Arrows indicate positions of the potent ASOs P12 and P13. (b) Northern blot analysis of PLK1 mRNA in MDA-MB-435 cells 24 h after ASO treatment. HSV-ASO served as negative control. To ascertain uniformity of loading the membrane was reprobed with human b-actin and G3PDH. Per cent inhibition was calculated by comparison with standardized PLK1 mRNA levels of cells grown in the absence of ASOs. PLK1 mRNA expression in HeLa S3 cells (c) and A549 cells (d) 24 h after ASO application. Membranes were reprobed with human b-actin
Article Snippet: Tumor cell lines MDA-MB-435 and A549 were obtained from CLS (Heidelberg),
Techniques: In Vitro, Hybridization, Northern Blot, Negative Control, Membrane, Inhibition, Comparison, Expressing
Journal: Oncogene
Article Title: Downregulation of human polo-like kinase activity by antisense oligonucleotides induces growth inhibition in cancer cells.
doi: 10.1038/sj.onc.1205412
Figure Lengend Snippet: Figure 2 Inhibition of PLK1 protein expression and kinase activity by PLK1-speci®c ASOs in cancer cells. Control cells were incubated with OPTIMEM. HSV-ASO served as negative control. Per cent inhibition was calculated by comparison with PLK1 protein levels in cells grown in the absence of ASOs. (a) Western blot analysis of PLK1 protein in MDA-MB-435 cells 48 h after ASO treatment. Indicated are the signals for PLK1 and actin in both autoradiographs. Actin served as control for equal loading (upper panel). Quanti®cation of PLK1 protein levels after normalization to actin was performed using a Kodak gel documentation system (1D 3.5). Mean values of at least 3 independent experiments with standard deviation are shown (lower panel). Quanti®cation of PLK1 protein in HeLa S3 cells (b) and A549 cells (c) after normalization to actin. PLK1 protein correlated to the concentration of ASO P12 in HeLa S3 cells (d) and in MDA-MB-435 cells (e). PLK1 immunoprecipitated from ASO treated cells phosphorylated casein to a lower extent compared to PLK1 from control cells (upper panel). Coomassie staining served as control for equal loading of substrate (middle panel). Equal amounts of PLK1 were subjected to the enzymatic assay (lower panel) (f)
Article Snippet: Tumor cell lines MDA-MB-435 and A549 were obtained from CLS (Heidelberg),
Techniques: Inhibition, Expressing, Activity Assay, Control, Incubation, Negative Control, Comparison, Western Blot, Standard Deviation, Concentration Assay, Immunoprecipitation, Staining, Enzymatic Assay
Journal: Oncogene
Article Title: Downregulation of human polo-like kinase activity by antisense oligonucleotides induces growth inhibition in cancer cells.
doi: 10.1038/sj.onc.1205412
Figure Lengend Snippet: Figure 3 Antiproliferative eects of PLK1 antisense inhibition in cancer cells. Control cells were incubated only with OPTIMEM. HSV-ASO served as a control. Percent inhibition was calculated by comparison to the number of cells treated with DOTAP grown in the absence of ASOs. (a) Growth inhibition of MDA-MB-435 cells treated with ASOs at a concentration of 250 nM over a period of 2 days. Growth inhibition of HeLa S3 (b) and A549 cells (c) over a period of 3 days. Dose-dependent reduction of MDA-MB-435 cells (d) and HeLa S3 cells (e) after ASO treatment. Mean values of at least three independent experiments with standard deviation are shown
Article Snippet: Tumor cell lines MDA-MB-435 and A549 were obtained from CLS (Heidelberg),
Techniques: Inhibition, Control, Incubation, Comparison, Concentration Assay, Standard Deviation