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Proteintech
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ATCC
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Image Search Results
Journal: bioRxiv
Article Title: Oncostatin M orchestrates collective epithelial migration via HIF1A activation
doi: 10.1101/2025.09.26.678830
Figure Lengend Snippet: (A) MCF10A cells were treated with EGF, OSM, or IFNG for 48 hours, then stained for DAPI (blue) and β-Catenin (red). (B) Cell velocity vectors represent speed and direction of cell motility, derived from cell tracking data during 24-48 hours after ligand treatment. Arrow size is proportional to velocity. (C-F) Quantification of cell phenotype from live-cell imaging. Shaded region represents 95% confidence interval from 3 biological replicates (n=3). Boxplots in (E) depict interquartile range of nearest neighbor distance calculated for each cell. (G-J) Line plots show protein expression levels normalized to the T0 control for each treatment condition. Error bars represent the full range of measurements (n=3).
Article Snippet: Afterward, cells were treated with either 10 ng/ml EGF (R&D Systems #236-EG), 10 ng/ml
Techniques: Staining, Derivative Assay, Cell Tracking Assay, Live Cell Imaging, Expressing, Control
Journal: bioRxiv
Article Title: Oncostatin M orchestrates collective epithelial migration via HIF1A activation
doi: 10.1101/2025.09.26.678830
Figure Lengend Snippet: (A) Workflow for the comparative network analysis to identify molecular subnetworks and nodes perturbed by OSM. Integrated molecular data collected from OSM and IFNG treated cells was analyzed using CausalPath. (B) Combined molecular network of OSM and IFNG activated nodes. The rewiring score between conditions is indicated by node color, and the top scoring nodes nominated for further investigation are indicated by increased size. (C) The top scoring nodes most rewired by OSM treatment, corresponding to the highlighted nodes in (B). (D) The majority (8/14) of top rewired nodes are centered around the STAT3 subnetwork.
Article Snippet: Afterward, cells were treated with either 10 ng/ml EGF (R&D Systems #236-EG), 10 ng/ml
Techniques:
Journal: Life
Article Title: Casomorphine-10 (CM-10) Peptide Orchestrates Circadian and Neurodevelopmental Gene Clusters via δ-Opioid Receptor Signaling: Insights from Transcriptome Analysis with δ-Opioid Receptor-Expressing HEK293 Cells
doi: 10.3390/life15101636
Figure Lengend Snippet: δ-opioid receptor (DOR)-expressing HEK293 cells and HEK293 cells stained with anti-DOR antibody and followed with FITC-conjugated anti-mouse IgG ( A ). Volcano plot of identified differentially expressed genes (DEGs) in DOR-HEK293 cells after 1 h treatment with CM-10 ( B ). Red boxes show changed gene expression over 1.4-time higher and less than 0.71 with p -value predicted by EdgeR of less than 0.05.
Article Snippet: The fixed DOR-HEK293 and
Techniques: Expressing, Staining, Gene Expression
Journal: Life
Article Title: Casomorphine-10 (CM-10) Peptide Orchestrates Circadian and Neurodevelopmental Gene Clusters via δ-Opioid Receptor Signaling: Insights from Transcriptome Analysis with δ-Opioid Receptor-Expressing HEK293 Cells
doi: 10.3390/life15101636
Figure Lengend Snippet: Predicted DOR agonistic signaling networks in DOR-HEK293 cells after 1 h treatment with CM-10. Fourteen genes with suggested involvement in cAMP-dependent protein kinases, transcriptional regulators in response to cAMP, circadian rhythm, stress and depression are shown in and were applied for network analysis by STRING. Red: circadian rhythm, Green: regulation of transcription of Notch receptor target, Yellow: PKA activation in glucagon signalling.
Article Snippet: The fixed DOR-HEK293 and
Techniques: Activation Assay