Journal: Cureus

Article Title: Topical Biological Agents as Adjuncts to Improve Wound Healing in Chronic Diabetic Wounds: A Systematic Review of Clinical Evidence and Future Directions

doi: 10.7759/cureus.27180

Figure Lengend Snippet: Summary of studies on human epidermal growth factor

Article Snippet: Tsang et al. (2003) [ ] , , 61 , Actovegin 5% cream and 0.02% hEGF, Actovegin 5% cream, and 0.04% hEGF , Actovegin 5% cream , 12 weeks , Proportion with complete wound healing, Wound healing rate , 20 of 21 patients (95.3%) in the 0.04% (wt/wt) hEGF group achieved complete healing.

Techniques: Control, Cream, Recombinant

Journal: Haematologica

Article Title: In vitro and in vivo evaluation of possible pro-survival activities of PGE2, EGF, TPO and FLT3L on human hematopoiesis

doi: 10.3324/haematol.2018.191569

Figure Lengend Snippet: Epidermal growth factor (EGF) does not protect human CD34 + from apoptosis induced by sublethal irradiation. (A) Cord blood-derived human CD34 + cells were treated for 24 and 48 hours (h) with etoposide (1 µg/mL) and different doses of human EGF (hEGF). Apoptosis was measured using AnnexinV/7AAD and specific apoptosis was calculated. Bars represent means±Standard Error of Mean (SEM) of 5 independent experiments. (B) Cord blood-derived human CD34 + cells were cultured for 24 and 48 h in the presence of human EGF and/or cetuximab at indicated concentrations and specific apoptosis was determined. Bars represent means±SEM of 4 independent experiments. (C) Human CD34 + cells were treated with both etoposide and cetuximab, and specific apoptosis was determined 24 h later. Bars represent means±SEM of 3 independent experiments. (D-F) Human CD34 + cells were transplanted into sublethally irradiated Rag2 −/− γc −/− mice. Four weeks later, mice were irradiated with 3 Gy or left untreated. Mice that were irradiated received daily injections of human and/or murine EGF. Eight days after irradiation, mice were sacrificed and % human CD45 + cells was determined in bone marrow (D) and spleen (E). In addition, the proportion of CD34 + immature cells was determined within the human cell population (F). Bars represent means±SEM of 4–7 animals from 5 independent experiments. Mann-Whitney test, * P ≤0.05; ** P ≤0.01; *** P ≤0.001).

Article Snippet: Thawed cells were cultured in serum-free medium supplemented with 10% ES-FBS (Invitrogen), human TPO (50 ng/mL, Immunotools), FLT3L, SCF, IL3 (100 ng/mL each, Immunotools), human EGF (hEGF) (20 or 200 ng/mL Immunotools), PGE2 (10, 25 or 50 μM, Sigma-Aldrich) and/or cytotoxic drugs (etoposide, taxol, tunicamycin; Sigma-Aldrich).

Techniques: Irradiation, Derivative Assay, Cell Culture, MANN-WHITNEY

Journal: Cell Death & Disease

Article Title: Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer

doi: 10.1038/cddis.2014.521

Figure Lengend Snippet: Formulations of media used in this study

Article Snippet: GBM complete media (for 276 BTICs) , 96% DMEM/F12 (Invitrogen, 11330-032) 2% Antibiotic/Antimycotic (Sigma, A5955) 2% B27 (Invitrogen, 17504-044) 20 ng/ml hFGF-b (PeproTech, 100-18B) 20 ng/ml hEGF (PeproTech, AF-100-15).

Techniques: Cell Culture