Journal: bioRxiv

Article Title: The mitochondrial NAD transporter SLC25A51 is a modulator of beta cell senescence and type 2 diabetes

doi: 10.1101/2025.04.09.647991

Figure Lengend Snippet: (A) ( A ) A diagram depicting the generation of SLC25A51-floxed C57BL/6N mice using a CRISPR-Cas9-based approach by Biocytogen Pharmaceuticals. ( B, C ) SLC25A51 expression per cell was compared between T2D patients and healthy controls using two different human beta-cell scRNA-seq datasets (E-MTAB-5061, GSE81608) ( Table S3 ). Heatmaps of Cdkn1a (p21) and Cdkn2a (p16) expression were also analyzed in both datasets. ( D ) 30-week-old SLC25A51-floxed C57BL/6N male mice were infused with either AAV6-INS1-Empty or AAV6-INS1-CRE viruses via pancreatic ductal infusion. Mice were sacrificed 15 weeks post-surgery. SLC25A51, p16 INK4A , MTCO2, and beta-actin protein expression levels were analyzed in pancreatic beta cells isolated from 45-week-old INS1-EMPTY (n=2) and INS1-CRE (n=4) groups. ( E-K ) Pancreatic ductal infusion of either PBS, AAV6-INS1-Empty, or AAV6-INS1-CRE was performed in each group of female C57BL/6N mice (n=5–6 per group). ( F ) Random blood glucose levels and ( G ) body weight were measured twice a week, and ( H ) beta-hydroxybutyrate levels were measured once a week for each mouse. ( I-K ) Six or eight weeks post-surgery, female mice underwent an IPGTT and ITT. Graphs show blood glucose levels at ( I, J ) each time point and area under the curve. ( I ) During the IPGTT, serum was collected from each group at 0 and 15 min to measure ( K ) serum insulin levels. Two weeks after the IPGTT, ( J ) an ITT was performed to test insulin sensitivity. Two-way ANOVA; error bars represent SEM; **p < 0.01.

Article Snippet: The data were visualized using heatmaps or dot-plot graphs in GraphPad Prism.

Techniques: CRISPR, Expressing, Isolation

Journal: Pharmaceuticals

Article Title: Computational Investigation of Montelukast and Its Structural Derivatives for Binding Affinity to Dopaminergic and Serotonergic Receptors: Insights from a Comprehensive Molecular Simulation

doi: 10.3390/ph18040559

Figure Lengend Snippet: Structural alignment and similarity analysis of Montelukast and its modifications. ( A ) Superimposition of Montelukast modifications with the native structure (backbone representation). ( B ) A 3D superimposition of Montelukast modifications with the native structure. Different colors represent different Montelukast modifications, allowing visual comparison of structural variations. ( C ) Pairwise similarity correlation heatmap, depicting the structural similarity scores between Montelukast and its modified derivatives. Higher similarity scores indicate minimal structural deviation, while lower scores suggest significant modifications. ( D ) Similarity score distribution plot, providing a comparative analysis of the degree of structural resemblance between each modified derivative and the native Montelukast. Purple colors indicate the similarity of each modification compared to the native structure of Montelukast.

Article Snippet: The similarity data were processed and visualized as a heatmap graph using OriginLab Pro version 2024 (OriginLab Corporation, Northampton, MA, USA) [ ].

Techniques: Comparison, Modification