Journal: BMJ Open Ophthalmology

Article Title: Protective effects of blue light-blocking shades on phototoxicity in human ocular surface cells

doi: 10.1136/bmjophth-2018-000217

Figure Lengend Snippet: Representative fluorescent microscopic images of immunostained cells with anti-pan-keratin antibody and its isotype control antibody (mouse IgG1). (A, B) Cells from human corneal surface origin (peripheral portion) and normal human dermal fibroblasts immunostained with anti-pan-keratin antibody, respectively. (C, D) Cells from human corneal surface origin (peripheral portion) and normal human dermal fibroblasts immunostained with an isotype control antibody (mouse IgG1), respectively. Scale bar=100 µm.

Article Snippet: Normal human dermal fibroblasts (NHDF, DS Pharma Biomedical, Osaka, Japan) were used as a negative control.

Techniques: Control

Journal: International Journal of Molecular Sciences

Article Title: Genetic Base of Behavioral Disorders in Mucopolysaccharidoses: Transcriptomic Studies

doi: 10.3390/ijms21031156

Figure Lengend Snippet: Characteristics of fibroblast lines derived from MPS patients and control cell lines.

Article Snippet: Non-transformed, patient-derived lines of MPS fibroblasts and HDFa were purchased from the NIGMS Human Genetic Cell Repository at the Coriell Institute for Medical Research.

Techniques: Derivative Assay, Control, Mutagenesis

Journal: International Journal of Molecular Sciences

Article Title: Genetic Base of Behavioral Disorders in Mucopolysaccharidoses: Transcriptomic Studies

doi: 10.3390/ijms21031156

Figure Lengend Snippet: Number of transcripts with changed levels of expression (at FDR < 0.1; p < 0.1 ( A ) or FDR < 0.000001; p < 0.1 ( B )) in different types of MPS relative to control cells (HDFa). FRD—false discovery rate; MPS—mucopolysaccharidosis.

Article Snippet: Non-transformed, patient-derived lines of MPS fibroblasts and HDFa were purchased from the NIGMS Human Genetic Cell Repository at the Coriell Institute for Medical Research.

Techniques: Expressing, Control

Journal: International Journal of Molecular Sciences

Article Title: Genetic Base of Behavioral Disorders in Mucopolysaccharidoses: Transcriptomic Studies

doi: 10.3390/ijms21031156

Figure Lengend Snippet: Number of up- and down-regulated transcripts of genes related to behavior (term GO:0007610 in the QuickGO database) in different types of MPS relative to control cells (HDFa).

Article Snippet: Non-transformed, patient-derived lines of MPS fibroblasts and HDFa were purchased from the NIGMS Human Genetic Cell Repository at the Coriell Institute for Medical Research.

Techniques: Control

Journal: International Journal of Molecular Sciences

Article Title: Genetic Base of Behavioral Disorders in Mucopolysaccharidoses: Transcriptomic Studies

doi: 10.3390/ijms21031156

Figure Lengend Snippet: Number of transcripts, corresponding to genes from indicated sub-processes (child processes) of behavior (term GO:0007610), defined according to the QuickGO database, which levels were significantly changed in MPS cells (MPS type or subtype is indicated in each panel) relative to the control cells. Fractions (%) of genes represented in each given GO term being differentially expressed relative to HDFa are also demonstrated above corresponding columns. Each sub-process is indicated by different color, according to the legend presented in the right-lower corner.

Article Snippet: Non-transformed, patient-derived lines of MPS fibroblasts and HDFa were purchased from the NIGMS Human Genetic Cell Repository at the Coriell Institute for Medical Research.

Techniques: Control

Journal: International Journal of Molecular Sciences

Article Title: Genetic Base of Behavioral Disorders in Mucopolysaccharidoses: Transcriptomic Studies

doi: 10.3390/ijms21031156

Figure Lengend Snippet: Genes which expression is significantly changed in at least six MPS types/subtypes relative to the control cells. Heat map of transcript levels is presented in panel ( A ) (MPS types and biological repeats are indicated). Comparison of results obtained for the OXTR gene using RNA-seq and RT-qPCR methods is shown in panel ( B ). Presented values are mean values from 4 experiments with SD shown as error bars. Statistically significant differences relative to control (HDFa) cells are indicated by asterisks.

Article Snippet: Non-transformed, patient-derived lines of MPS fibroblasts and HDFa were purchased from the NIGMS Human Genetic Cell Repository at the Coriell Institute for Medical Research.

Techniques: Expressing, Control, Comparison, RNA Sequencing, Quantitative RT-PCR

Journal: International Journal of Molecular Sciences

Article Title: Genetic Base of Behavioral Disorders in Mucopolysaccharidoses: Transcriptomic Studies

doi: 10.3390/ijms21031156

Figure Lengend Snippet: Changes in expression (transcripts’ levels) of particular genes in MPS types are depicted, indicating log 2 fold change (FC) relative to control cells (HDFa); up-regulation is marked in bold and down-regulation in marked in italic, while not statistically significant differences are not marked.

Article Snippet: Non-transformed, patient-derived lines of MPS fibroblasts and HDFa were purchased from the NIGMS Human Genetic Cell Repository at the Coriell Institute for Medical Research.

Techniques: Expressing, Control

Journal: International Journal of Molecular Sciences

Article Title: Genetic Base of Behavioral Disorders in Mucopolysaccharidoses: Transcriptomic Studies

doi: 10.3390/ijms21031156

Figure Lengend Snippet: Volcano plots indicating genes which expression is significantly changed in MPS types relative to control cells (HDFa) and reached log 2 FC > 2.5. The gene ( MME ) for which two alternative transcripts were changed in the above manner is indicated in MPS IIID.

Article Snippet: Non-transformed, patient-derived lines of MPS fibroblasts and HDFa were purchased from the NIGMS Human Genetic Cell Repository at the Coriell Institute for Medical Research.

Techniques: Expressing, Control

Journal: International Journal of Molecular Sciences

Article Title: Genetic Base of Behavioral Disorders in Mucopolysaccharidoses: Transcriptomic Studies

doi: 10.3390/ijms21031156

Figure Lengend Snippet: Genes which expression is particularly significantly changed (log 2 FC > 2.5) in different MPS types/subtypes relative to control cells – heat maps. Heat maps of transcript levels are presented for each MPS type (types: I, II, IIIA, IIIB, IIIC, IIID, IVA, IVB, VI, VII and IX in panels A – K , respectively), relative to control cell line (HDFa), with 4 biological repeats indicated in each case. Names of genes are indicated at the right side of each panel. The scale of changes in shown below panel K.

Article Snippet: Non-transformed, patient-derived lines of MPS fibroblasts and HDFa were purchased from the NIGMS Human Genetic Cell Repository at the Coriell Institute for Medical Research.

Techniques: Expressing, Control

Journal: International Journal of Molecular Sciences

Article Title: Genetic Base of Behavioral Disorders in Mucopolysaccharidoses: Transcriptomic Studies

doi: 10.3390/ijms21031156

Figure Lengend Snippet: Genes which expression is particularly significantly changed (log 2 FC > 2.5) in different MPS types/subtypes relative to control cells—quantitative analysis. Changes in levels of particular transcripts are presented for each MPS type relative to control cell line (HDFa). Names of genes are indicated. Down-regulation is marked in blue, and up-regulation in red. Presented values are mean values from 4 experiments with SD shown as error bars. Statistically significant differences relative to control (HDFa) cells are indicated by asterisks.

Article Snippet: Non-transformed, patient-derived lines of MPS fibroblasts and HDFa were purchased from the NIGMS Human Genetic Cell Repository at the Coriell Institute for Medical Research.

Techniques: Expressing, Control

Journal: International Journal of Molecular Sciences

Article Title: Genetic Base of Behavioral Disorders in Mucopolysaccharidoses: Transcriptomic Studies

doi: 10.3390/ijms21031156

Figure Lengend Snippet: Comparison of results of assessing levels of transcripts using RNA-seq and RT-qPCR methods, obtained for selected genes in all tested MPS types/subtypes relative to control cell line (HDFa). One gene was tested in each MPS type/subtype as indicated in the figure. Presented values are mean values from 4 experiments with SD shown as error bars. Statistically significant differences relative to control (HDFa) cells are indicated by asterisks.

Article Snippet: Non-transformed, patient-derived lines of MPS fibroblasts and HDFa were purchased from the NIGMS Human Genetic Cell Repository at the Coriell Institute for Medical Research.

Techniques: Comparison, RNA Sequencing, Quantitative RT-PCR, Control

Journal: International Journal of Molecular Sciences

Article Title: Genetic Base of Behavioral Disorders in Mucopolysaccharidoses: Transcriptomic Studies

doi: 10.3390/ijms21031156

Figure Lengend Snippet: RT-qPCR analysis of levels of transcripts of selected genes ( THBS1 and OXTR ) in control 2 (healthy female) and control 3 ( IDUA heterozygote) fibroblasts, as well as in MPS II fibroblast, either untreated or treated with human recombinant iduronate-2-sulfatase (Elaprase, IDS) at 0.5 mg/mL for 24 h, relative to HDFa cells. Presented values are mean values from 3 experiments with SD shown as error bars. Statistically significant differences relative to control cells (HDFa) are indicated by asterisks.

Article Snippet: Non-transformed, patient-derived lines of MPS fibroblasts and HDFa were purchased from the NIGMS Human Genetic Cell Repository at the Coriell Institute for Medical Research.

Techniques: Quantitative RT-PCR, Control, Recombinant