Journal: Advanced Healthcare Materials

Article Title: Panthenol Citrate Biomaterials Accelerate Wound Healing and Restore Tissue Integrity

doi: 10.1002/adhm.202301683

Figure Lengend Snippet: PC and PC‐PPCN promote the migration and proliferation of human dermal fibroblasts (HDF) and human epidermal keratinocytes (HEK α ) from healthy and diabetic individuals. a) Images of healthy HDF migration after exposure to saline, PC, PPCN, and PC‐PPCN for 8 h. b) Quantification of percentage wound area remaining for healthy HDF. c) Healthy HDF proliferation at 72 h. d) Images of diabetic HDF migration after exposure to saline, PC, PPCN, and PC‐PPCN for 8 h. e) Quantification of percentage of wound area remaining for diabetic HDF. f) Diabetic HDF proliferation at 72 h. g) Images of healthy HEK α migration after exposure to saline, PC, PPCN, and PC‐PPCN for 24 h. h) Quantification of percentage of wound area remaining for healthy HEK α . i) Healthy HEK α proliferation at 72 h. j) Images of diabetic HEK α migration after exposure to saline, PC, PPCN, and PC‐PPCN for 24 h. k) Quantification of percentage of wound area remaining for diabetic HEK α . l) Diabetic HEK α proliferation at 72 h ( n = 3; ns, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001, **** p < 0.0001).

Article Snippet: Healthy and diabetic HEK α and HDF (Zen‐bio, passage 1–2), and HMVEC (Lonza, passage 1–2) proliferation assays were conducted by seeding cells at a density of 5 × 10 4 cells per well in 24‐well tissue culture plates and incubated overnight to allow cell attachment.

Techniques: Migration, Saline

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Assessment of chemotherapeutic effects on cancer cells using adhesion noise spectroscopy

doi: 10.3389/fbioe.2024.1385730

Figure Lengend Snippet: 5-Fluorouracil (5-FU) with cytotoxic and detachment-enhancing effect on the colorectal cancer cell (CRC) line HT-29 but without effect on human dermal fibroblast (HDF) cell viability and proliferation. (A) 5-FU effects via CAN spectroscopy on HT-29 cells cultivated on the CMOS MEA (analyzed at 300 kHz). Untreated HT-29 grew on the CMOS MEA and the cell-covered area rose (A-i) . After 5-FU treatment, the detection positive area declined after 72 h of cultivation (A-ii) . Statistics (A-i,A-ii) : one-way ANOVA. Statistical difference of the cell-covered area between untreated and treated HT-29 (A-iii) . Statistics (A-iii) : two-way ANOVA. (B) 5-FU effects via CAN spectroscopy on HDF cells cultivated on the CMOS MEA (analyzed at 300 kHz). Untreated and treated HDFs showed a stagnant CMOS MEA area where cells are adhered to (B-i,B-ii) . Statistics (B-i,B-ii) : one-way ANOVA. CMOS MEA area covered by HDFs (B-iii) . Statistics (B-iii) : two-way ANOVA. (C) 5-FU effects on HT-29 cells via CCK-8 assay. Untreated HT-29 cells’ optical density (OD) increased during 72 h cultivation time (C-i) , while 5-FU treated cells’ OD declined (C-ii) . (D) 5-FU effects on HDF cells via CCK-8 assay. For HDFs, the OD stays constant without (D-i) and with 5-FU treatment (D-ii) . Statistics: one-way ANOVA. (C-iii,D-iii) compared cell viability of treated cells with untreated cells as control group. Treated cells showed less cell viability for both cell types [HT-29 (C-iii) and HDF (D-iii) ]. Statistics: two-way ANOVA. (E) Cell viability from CASY recordings of treated and untreated HT-29 in the supernatant and on the chip (E-i) . Same for HDFs (E-ii) . Two-way ANOVA. Statistical significance is indicated by asterisks (n HT-29 = 4, n HDF = 4, ns, not significant. *, p ≤ 0.05. **, p ≤ 0.01. ***, p ≤ 0.001. ****, p ≤ 0.0001).

Article Snippet: The colorectal cancer (CRC) cell line HT-29 (ATCC, RRID: CVCL_0320) and the human dermal fibroblast cell line HDF (PELOBiotech GmbH, RRID: CVCL_DP66) were cultivated at 37°C in a 5% CO 2 atmosphere.

Techniques: Spectroscopy, CCK-8 Assay, Control