hckα Search Results


anti-hck  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc anti-hck
A. NK-92 cells were treated for 10 minutes with IL-12 and/or ISG15 and total cell extracts were analyzed by immunoblotting with an antibody that recognizes the phosphorylated and activated form of three SRC family kinases expressed in NK-92 <t>cells</t> <t>(LYN,</t> <t>HCK,</t> FGR). B. As in (A.), phosphorylated and activated SFKs were analyzed by immunoblotting in response to the indicated ISG15 variants. C. IFN-γ ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM), an inhibitor of LYN, HCK, and FGR. Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. D. IL-10 ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM). Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. See also Figure S6.
Anti Hck, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-hck a2083  (ABclonal Biotechnology)
90
ABclonal Biotechnology anti-hck a2083
A. NK-92 cells were treated for 10 minutes with IL-12 and/or ISG15 and total cell extracts were analyzed by immunoblotting with an antibody that recognizes the phosphorylated and activated form of three SRC family kinases expressed in NK-92 <t>cells</t> <t>(LYN,</t> <t>HCK,</t> FGR). B. As in (A.), phosphorylated and activated SFKs were analyzed by immunoblotting in response to the indicated ISG15 variants. C. IFN-γ ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM), an inhibitor of LYN, HCK, and FGR. Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. D. IL-10 ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM). Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. See also Figure S6.
Anti Hck A2083, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-hck a2083/product/ABclonal Biotechnology
Average 90 stars, based on 1 article reviews
anti-hck a2083 - by Bioz Stars, 2026-03
90/100 stars
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anti-phospho-hck tyr-411  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology anti-phospho-hck tyr-411
A. NK-92 cells were treated for 10 minutes with IL-12 and/or ISG15 and total cell extracts were analyzed by immunoblotting with an antibody that recognizes the phosphorylated and activated form of three SRC family kinases expressed in NK-92 <t>cells</t> <t>(LYN,</t> <t>HCK,</t> FGR). B. As in (A.), phosphorylated and activated SFKs were analyzed by immunoblotting in response to the indicated ISG15 variants. C. IFN-γ ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM), an inhibitor of LYN, HCK, and FGR. Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. D. IL-10 ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM). Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. See also Figure S6.
Anti Phospho Hck Tyr 411, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-phospho-hck tyr-411/product/Santa Cruz Biotechnology
Average 90 stars, based on 1 article reviews
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90/100 stars
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anti-src  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc anti-src
A. NK-92 cells were treated for 10 minutes with IL-12 and/or ISG15 and total cell extracts were analyzed by immunoblotting with an antibody that recognizes the phosphorylated and activated form of three SRC family kinases expressed in NK-92 <t>cells</t> <t>(LYN,</t> <t>HCK,</t> FGR). B. As in (A.), phosphorylated and activated SFKs were analyzed by immunoblotting in response to the indicated ISG15 variants. C. IFN-γ ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM), an inhibitor of LYN, HCK, and FGR. Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. D. IL-10 ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM). Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. See also Figure S6.
Anti Src, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-src/product/Cell Signaling Technology Inc
Average 90 stars, based on 1 article reviews
anti-src - by Bioz Stars, 2026-03
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hckα  (OriGene)
90
OriGene hckα
A. NK-92 cells were treated for 10 minutes with IL-12 and/or ISG15 and total cell extracts were analyzed by immunoblotting with an antibody that recognizes the phosphorylated and activated form of three SRC family kinases expressed in NK-92 <t>cells</t> <t>(LYN,</t> <t>HCK,</t> FGR). B. As in (A.), phosphorylated and activated SFKs were analyzed by immunoblotting in response to the indicated ISG15 variants. C. IFN-γ ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM), an inhibitor of LYN, HCK, and FGR. Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. D. IL-10 ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM). Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. See also Figure S6.
Hckα, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hckα/product/OriGene
Average 90 stars, based on 1 article reviews
hckα - by Bioz Stars, 2026-03
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anti-p59 hck  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology anti-p59 hck
A. NK-92 cells were treated for 10 minutes with IL-12 and/or ISG15 and total cell extracts were analyzed by immunoblotting with an antibody that recognizes the phosphorylated and activated form of three SRC family kinases expressed in NK-92 <t>cells</t> <t>(LYN,</t> <t>HCK,</t> FGR). B. As in (A.), phosphorylated and activated SFKs were analyzed by immunoblotting in response to the indicated ISG15 variants. C. IFN-γ ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM), an inhibitor of LYN, HCK, and FGR. Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. D. IL-10 ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM). Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. See also Figure S6.
Anti P59 Hck, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-p59 hck/product/Santa Cruz Biotechnology
Average 90 stars, based on 1 article reviews
anti-p59 hck - by Bioz Stars, 2026-03
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anti-hck (n-30  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology anti-hck (n-30
A. NK-92 cells were treated for 10 minutes with IL-12 and/or ISG15 and total cell extracts were analyzed by immunoblotting with an antibody that recognizes the phosphorylated and activated form of three SRC family kinases expressed in NK-92 <t>cells</t> <t>(LYN,</t> <t>HCK,</t> FGR). B. As in (A.), phosphorylated and activated SFKs were analyzed by immunoblotting in response to the indicated ISG15 variants. C. IFN-γ ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM), an inhibitor of LYN, HCK, and FGR. Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. D. IL-10 ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM). Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. See also Figure S6.
Anti Hck (N 30, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-hck (n-30/product/Santa Cruz Biotechnology
Average 90 stars, based on 1 article reviews
anti-hck (n-30 - by Bioz Stars, 2026-03
90/100 stars
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pcmv6 hckα  (OriGene)
90
OriGene pcmv6 hckα
A. NK-92 cells were treated for 10 minutes with IL-12 and/or ISG15 and total cell extracts were analyzed by immunoblotting with an antibody that recognizes the phosphorylated and activated form of three SRC family kinases expressed in NK-92 <t>cells</t> <t>(LYN,</t> <t>HCK,</t> FGR). B. As in (A.), phosphorylated and activated SFKs were analyzed by immunoblotting in response to the indicated ISG15 variants. C. IFN-γ ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM), an inhibitor of LYN, HCK, and FGR. Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. D. IL-10 ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM). Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. See also Figure S6.
Pcmv6 Hckα, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcmv6 hckα/product/OriGene
Average 90 stars, based on 1 article reviews
pcmv6 hckα - by Bioz Stars, 2026-03
90/100 stars
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anti-hck eab-14119  (Elabscience Biotechnology)
90
Elabscience Biotechnology anti-hck eab-14119
A. NK-92 cells were treated for 10 minutes with IL-12 and/or ISG15 and total cell extracts were analyzed by immunoblotting with an antibody that recognizes the phosphorylated and activated form of three SRC family kinases expressed in NK-92 <t>cells</t> <t>(LYN,</t> <t>HCK,</t> FGR). B. As in (A.), phosphorylated and activated SFKs were analyzed by immunoblotting in response to the indicated ISG15 variants. C. IFN-γ ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM), an inhibitor of LYN, HCK, and FGR. Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. D. IL-10 ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM). Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. See also Figure S6.
Anti Hck Eab 14119, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-hck eab-14119/product/Elabscience Biotechnology
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anti-hck eab-14119 - by Bioz Stars, 2026-03
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hck  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology hck
A. NK-92 cells were treated for 10 minutes with IL-12 and/or ISG15 and total cell extracts were analyzed by immunoblotting with an antibody that recognizes the phosphorylated and activated form of three SRC family kinases expressed in NK-92 <t>cells</t> <t>(LYN,</t> <t>HCK,</t> FGR). B. As in (A.), phosphorylated and activated SFKs were analyzed by immunoblotting in response to the indicated ISG15 variants. C. IFN-γ ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM), an inhibitor of LYN, HCK, and FGR. Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. D. IL-10 ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM). Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. See also Figure S6.
Hck, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hck/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
hck - by Bioz Stars, 2026-03
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anti-hck  (Becton Dickinson)
90
Becton Dickinson anti-hck
A. NK-92 cells were treated for 10 minutes with IL-12 and/or ISG15 and total cell extracts were analyzed by immunoblotting with an antibody that recognizes the phosphorylated and activated form of three SRC family kinases expressed in NK-92 <t>cells</t> <t>(LYN,</t> <t>HCK,</t> FGR). B. As in (A.), phosphorylated and activated SFKs were analyzed by immunoblotting in response to the indicated ISG15 variants. C. IFN-γ ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM), an inhibitor of LYN, HCK, and FGR. Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. D. IL-10 ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM). Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. See also Figure S6.
Anti Hck, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-hck/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
anti-hck - by Bioz Stars, 2026-03
90/100 stars
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anti hck  (Proteintech)
93
Proteintech anti hck
A. NK-92 cells were treated for 10 minutes with IL-12 and/or ISG15 and total cell extracts were analyzed by immunoblotting with an antibody that recognizes the phosphorylated and activated form of three SRC family kinases expressed in NK-92 <t>cells</t> <t>(LYN,</t> <t>HCK,</t> FGR). B. As in (A.), phosphorylated and activated SFKs were analyzed by immunoblotting in response to the indicated ISG15 variants. C. IFN-γ ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM), an inhibitor of LYN, HCK, and FGR. Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. D. IL-10 ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM). Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. See also Figure S6.
Anti Hck, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti hck/product/Proteintech
Average 93 stars, based on 1 article reviews
anti hck - by Bioz Stars, 2026-03
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Image Search Results


A. NK-92 cells were treated for 10 minutes with IL-12 and/or ISG15 and total cell extracts were analyzed by immunoblotting with an antibody that recognizes the phosphorylated and activated form of three SRC family kinases expressed in NK-92 cells (LYN, HCK, FGR). B. As in (A.), phosphorylated and activated SFKs were analyzed by immunoblotting in response to the indicated ISG15 variants. C. IFN-γ ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM), an inhibitor of LYN, HCK, and FGR. Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. D. IL-10 ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM). Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. See also Figure S6.

Journal: Molecular cell

Article Title: Extracellular ISG15 Signals Cytokine Secretion Through the LFA-1 Integrin Receptor

doi: 10.1016/j.molcel.2017.10.003

Figure Lengend Snippet: A. NK-92 cells were treated for 10 minutes with IL-12 and/or ISG15 and total cell extracts were analyzed by immunoblotting with an antibody that recognizes the phosphorylated and activated form of three SRC family kinases expressed in NK-92 cells (LYN, HCK, FGR). B. As in (A.), phosphorylated and activated SFKs were analyzed by immunoblotting in response to the indicated ISG15 variants. C. IFN-γ ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM), an inhibitor of LYN, HCK, and FGR. Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. D. IL-10 ELISA of NK-92 cell culture supernatants in the presence of increasing amounts of ISG15, with and without addition of PP2 (10 μM). Three biological repeats of the experiment were conducted and the error bars represent the standard error of the mean. See also Figure S6.

Article Snippet: Other antibodies and their sources were: anti-M2 FLAG antibody (Sigma), anti-phospho LCK Y394 (Thermo scientific), anti-LYN (Cell Signaling Technologies), anti-HCK (Cell Signaling Technologies), anti-FGR (Cell Signaling Technologies) , anti-phospho Src family Y416 (recognizing phosphor-LYN, HCK, and FGR; Cell Signaling Technologies) , anti-M2 Flag Beads (Sigma), Glutathione Sepharose (GE healthcare).

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Cell Culture

Key Resources Table

Journal: Molecular cell

Article Title: Extracellular ISG15 Signals Cytokine Secretion Through the LFA-1 Integrin Receptor

doi: 10.1016/j.molcel.2017.10.003

Figure Lengend Snippet: Key Resources Table

Article Snippet: Other antibodies and their sources were: anti-M2 FLAG antibody (Sigma), anti-phospho LCK Y394 (Thermo scientific), anti-LYN (Cell Signaling Technologies), anti-HCK (Cell Signaling Technologies), anti-FGR (Cell Signaling Technologies) , anti-phospho Src family Y416 (recognizing phosphor-LYN, HCK, and FGR; Cell Signaling Technologies) , anti-M2 Flag Beads (Sigma), Glutathione Sepharose (GE healthcare).

Techniques: Clone Assay, Expressing, Recombinant, Enzyme-linked Immunosorbent Assay, Software