Journal: Molecular Medicine Reports

Article Title: X-irradiation induces acute and early term inflammatory responses in atherosclerosis-prone ApoE − / − mice and in endothelial cells

doi: 10.3892/mmr.2021.12038

Figure Lengend Snippet: Inflammatory response in endothelial cells at 24 h after irradiation. The response of various inflammatory markers at 24 h after 0.1 and 5 Gy irradiation is represented in (A-I) TICAE cells and (J-R) TIME cells, normalized to cell count. The Kruskal-Wallis test was used to analyse the data and the P-value was adjusted using the Benjamini-Hochberg method. Values represent the average ± SEM of 6 biological replicates. *P<0.05, **P<0.01 and ***P<0.001 vs. 0 Gy. p.i., post irradiation; GDF-15, growth differentiation factor-15; CXCL10, C-X-C motif chemokine ligand 10; ICAM-1, intercellular adhesion molecule-1; MCP-1, monocyte chemoattractant protein-1; uPAR, urokinase-type plasminogen activator receptor; PAI-1, plasminogen activator inhibitor-1; FGF-basic, basic fibroblast growth factor.

Article Snippet: In addition, TICAE cells, which are primary human coronary artery endothelial cells from the European Collection of Authenticated Cell Cultures (ECACC; HCAECs cat. no. 300-05a) that were transduced with retroviruses bearing the est2 gene, a yeast homologue of the human TERT protein ( , ), were used.

Techniques: Irradiation, Cell Counting

Journal: Molecular Medicine Reports

Article Title: X-irradiation induces acute and early term inflammatory responses in atherosclerosis-prone ApoE − / − mice and in endothelial cells

doi: 10.3892/mmr.2021.12038

Figure Lengend Snippet: Inflammatory response in endothelial cells at 72 h after irradiation. The response of various inflammatory markers at 72 h after 0.1 and 5 Gy irradiation is presented in (A-I) TICAE cells and (J-R) TIME cells. Data were normalized to cell count. The Kruskal-Wallis test was used to analyse the data and the P-value was adjusted using the Benjamini-Hochberg method. Values represent the average ± SEM of 6 biological replicates. *P<0.05, **P<0.01 and ***P<0.001 vs. 0 Gy. p.i., post irradiation; GDF-15, growth differentiation factor-15; CXCL10, C-X-C motif chemokine ligand 10; ICAM-1, intercellular adhesion molecule-1; MCP-1, monocyte chemoattractant protein-1; uPAR, urokinase-type plasminogen activator receptor; PAI-1, plasminogen activator inhibitor-1; FGF-basic, basic fibroblast growth factor.

Article Snippet: In addition, TICAE cells, which are primary human coronary artery endothelial cells from the European Collection of Authenticated Cell Cultures (ECACC; HCAECs cat. no. 300-05a) that were transduced with retroviruses bearing the est2 gene, a yeast homologue of the human TERT protein ( , ), were used.

Techniques: Irradiation, Cell Counting

Journal:

Article Title: Eotaxin Increases Monolayer Permeability of Human Coronary Artery Endothelial Cells

doi: 10.1161/ATVBAHA.109.194134

Figure Lengend Snippet: Effects of eotaxin, antimycin A, MnTBAP, ginkgolide B and CCR3 on endothelial monolayer permeability in HCAECs. Endothelial monolayer permeability was analyzed with a costar transwell system and a Texas Red-labeled dextran tracer. A, Concentration-dependent study. HCAECs were treated with increasing concentrations of eotaxin (50, 100 and 200 ng/mL), TNF-α (2 ng/mL) or with ginkgolide B (5 μM) for 24 h. n=3, *p<0.05 B, Effects of CCR3 receptor blocking and heat-inactivation (HI) of eotaxin on endothelial permeability. HCAECs were treated with eotaxin (100 ng/mL) or HI-eotaxin (100 gm/mL) with or without anti-CCR3 antibody 24 h. n=3, *p<0.05. C, Effect of antimycin A on endothelial permeability. HCAECs were treated with antimycin A (10 μg/mL) for 24 h in the presence or absence of antioxidant MnTBAP (2 μM). n=3, *p<0.05. D, Western blot analysis. HCAECs were treated with eotaxin (100 ng/mL) and/or MnTBAP (2 μM) for 24 h and CCR3 protein levels were determined by Western blot. Loading efficiency was determined by reprobing the blot with β-actin antibody. Experiments were repeated twice. Statistical comparison is indicated by the arrow head. Error bars represent SD.

Article Snippet: Endothelial Permeability HCAECs were obtained from Gelantis (San Diego, CA) and cultured in HCAEC Growth medium (Gelantis).

Techniques: Permeability, Labeling, Concentration Assay, Blocking Assay, Western Blot, Comparison

Journal:

Article Title: Eotaxin Increases Monolayer Permeability of Human Coronary Artery Endothelial Cells

doi: 10.1161/ATVBAHA.109.194134

Figure Lengend Snippet: Effects of eotaxin on the expression of junctional molecules in HCAECs. HCAECs were treated with serial concentrations of eotaxin (50, 100 and 200 ng/mL) for 24 h. A, The mRNA levels of ZO-1, claudin-1, occludin, JAM-1 and VE cadherin were analyzed by real time PCR, and values were normalized to GAPDH [2(Ct GAPDH −Ct gene of interest)] in each sample. n=3 *p<0.05 (compared with controls). B, The protein levels of ZO-1, occludin, claudin-1, VE cadherin and JAM-1 were analyzed by Western blot analysis. Loading efficiency was determined by reprobing the blot with β-actin antibody. Eotaxin and antioxidant MnTBAP were used. Experiments were repeated twice. C, Histogram of flow cytometry analysis showing protein levels of ZO-1, claudin-1, and occludin under the control and eotaxin-treated conditions. HCAECs were treated with 100 ng/mL eotaxin for 24 h. Experiments were repeated twice.

Article Snippet: Endothelial Permeability HCAECs were obtained from Gelantis (San Diego, CA) and cultured in HCAEC Growth medium (Gelantis).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Flow Cytometry, Control

Journal:

Article Title: Eotaxin Increases Monolayer Permeability of Human Coronary Artery Endothelial Cells

doi: 10.1161/ATVBAHA.109.194134

Figure Lengend Snippet: Time course study of the effect of eotaxin on mRNA levels of tight junction molecules in HCAECs. Cells were treated with eotaxin (100 ng/mL) for different time points (0, 45 min, 2 h, 6 h, 18 h and 24 h) and the mRNA levels of ZO-1 (A), occludin (B) and claudin-1 (C) were determined by real-time PCR and values were normalized to GAPDH [2(Ct GAPDH −Ct gene of interest)] in each sample. n=3, *p<0.05 (compared with controls). Error bars represent SD.

Article Snippet: Endothelial Permeability HCAECs were obtained from Gelantis (San Diego, CA) and cultured in HCAEC Growth medium (Gelantis).

Techniques: Real-time Polymerase Chain Reaction

Journal:

Article Title: Eotaxin Increases Monolayer Permeability of Human Coronary Artery Endothelial Cells

doi: 10.1161/ATVBAHA.109.194134

Figure Lengend Snippet: Effects of eotaxin, antimycin A, TNF-α, and MnTBAP on the cellular glutathione (GSH) levels in HCAECs. Cells were treated with eotaxin (100 ng/mL), antimycin A (10 μg/mL), TNF-α (2 ng/mL) and/or MnTBAP (2 μM) for 45 min. Cellular GSH levels were determined by a GSH-Glo Glutathione assay kit. n=3. *p<005 (the comparison is indicated by the arrow head). Error bars represent SD.

Article Snippet: Endothelial Permeability HCAECs were obtained from Gelantis (San Diego, CA) and cultured in HCAEC Growth medium (Gelantis).

Techniques: Glutathione Assay, Comparison

Journal:

Article Title: Eotaxin Increases Monolayer Permeability of Human Coronary Artery Endothelial Cells

doi: 10.1161/ATVBAHA.109.194134

Figure Lengend Snippet: Effects of eotaxin, antimycin A and MnTBAP on the activation of p38, Stat3 and NFκB in HCAECs. A, Phosphorylation of p38 and Stat3 as well as nuclear translocation of NF-kB p65 in HCAECs. Cells were treated with either eotaxin (100 ng/mL) or antimycin A (10 μg/mL) for 45 min or pretreated with MnTBAP (2 μM) for 30 min followed by antimycin A treatment for 30 min. Cytoplasmic and nuclear extracts were isolated, respectively. Phosphorylated (p) and total p38 and Stat3 as well as NF-kB p65 proteins were detected by the Western blotting analysis. Loading efficiency was determined by reprobing the blot with β-actin antibody. The experiment was repeated twice. B, Endothelial permeability. HCAECs were treated with eotaxin (100 ng/mL) alone or pretreated with specific p38 inhibitor SB203580 followed by eotaxin treatment for 24 h. Endothelial permeability was assayed. n=3. *p<0.05 (the comparison is indicated by the arrow head). Error bars represent SD.

Article Snippet: Endothelial Permeability HCAECs were obtained from Gelantis (San Diego, CA) and cultured in HCAEC Growth medium (Gelantis).

Techniques: Activation Assay, Phospho-proteomics, Translocation Assay, Isolation, Western Blot, Permeability, Comparison