Journal: Advanced Healthcare Materials

Article Title: Plasma‐Polymerized Nanoparticles Presenting Fibrillin‐1 Drive Rapid Re‐Endothelialization of Vascular Grafts

doi: 10.1002/adhm.202503360

Figure Lengend Snippet: PPN‐PF8 functionalization of ePTFE improves endothelial attachment in vitro. A) Representative images of SEM and F‐actin staining. B) HCAEC attachment and C) spreading on ePTFE, ePTFE with passively bound PF8 (ePTFE‐PF8), PPN‐coated ePTFE (PPN), and PPN‐PF8 functionalized ePTFE, n = 3 per sample. Scale bar = 300 µm. * p < 0.05, *** p < 0.001 versus ePTFE.

Article Snippet: Human coronary artery endothelial cells (HCAEC; Cell Applications) were cultured in MesoEndo medium (Cell Applications) at 37 °C in 5% CO 2 .

Techniques: In Vitro, Staining

Journal: Advanced Healthcare Materials

Article Title: Plasma‐Polymerized Nanoparticles Presenting Fibrillin‐1 Drive Rapid Re‐Endothelialization of Vascular Grafts

doi: 10.1002/adhm.202503360

Figure Lengend Snippet: PPN‐PF8 functionalization of ePTFE improves endothelial proliferation in vitro. A) Representative images of SEM and F‐actin staining of HCAEC proliferation on days 1 and 3. B) HCAEC proliferation and C) spreading on ePTFE, ePTFE with passively bound PF8, PPN, and PPN‐PF8 functionalized ePTFE. * p < 0.05, *** p < 0.001 versus ePTFE on each day, Scale bar = 300 µm. n = 3 per sample.

Article Snippet: Human coronary artery endothelial cells (HCAEC; Cell Applications) were cultured in MesoEndo medium (Cell Applications) at 37 °C in 5% CO 2 .

Techniques: In Vitro, Staining

Journal: Circulation. Cardiovascular interventions

Article Title: Impact of stent surface on thrombogenicity and vascular healing: a comparative analysis of metallic and polymeric surfaces.

doi: 10.1161/CIRCINTERVENTIONS.113.000120

Figure Lengend Snippet: Figure 4. Endothelialization of uncoated and polyvinylidene flu- oride-co-hexafluoropropene (PVDF-HFP)–coated platinum chro- mium (PtCr) stents. A, Absorbance values of CD31 expression (minus background) for endothelial cells (ECs) covering stents at 7 (n=9) and 14 days (n=7). At 7 (P<0.01) and 14 (P=0.02) days, there were significant differences between the endothelialization between uncoated and PVDF-HFP–coated PtCr stents. Values shown are mean±SEM . B, Human coronary artery EC coverage on PVDF-HFP at 7 (n=9) and 14 days (n=7) was normalized to the respective value for PtCr at each time point. Endothelialization of uncoated PtCr stents was greater at 7 and 14 days compared with that on PVDF-HFP–coated stents (P<0.01). Values shown are mean with 95% confidence interval. *Significantly different from PtCr at the respective time point (P<0.05).

Article Snippet: Endothelial cell (EC) growth on uncoated and PVDF-HFP–coated PtCr stents (3.0 mm×16 mm, ELEMENT) was evaluated using human coronary artery ECs (HCAECs; Cell Applications Inc, San Diego, CA), as previously described,11 and is described in detail in the online-only Data Supplement.

Techniques: Expressing

Journal: Circulation. Cardiovascular interventions

Article Title: Impact of stent surface on thrombogenicity and vascular healing: a comparative analysis of metallic and polymeric surfaces.

doi: 10.1161/CIRCINTERVENTIONS.113.000120

Figure Lengend Snippet: Figure 5. Representative micrographs of immunohistochemical staining of human coronary artery endothelial cells (HCAECs) on uncoated and polyvinylidene fluoride-co-hexafluoropropene (PVDF-HFP)–coated platinum chromium (PtCr) stents at 14 days. 4',6-diamidino-2-phenylindole (DAPI)–stained nuclei of HCAEC on (A) uncoated and (D) PVDF-HFP–coated stents demonstrate presence of cells at 14 days with greater levels on uncoated stents. Expression of vascular endothelial (VE)-cadherin at intracellular junctions of HCAECs on (B) uncoated stents is readily evident and reflects typical cobblestone morphology of endothelial cells (within circle). In contrast, VE-cadherin expression on HCAEC covering (D) PVDF-HFP–coated stents is relatively weaker at intracellular junctions (within circle), sug- gesting reduced barrier function compared with HCAEC covering uncoated stents. For comparison on uncoated PtCr stents, (H) an isotype control for VE-cadherin displays nominal back- ground staining. C, F, and I, DAPI and VE-cadherin images were merged with the transmitted images of the underlying stents to represent strut location.

Article Snippet: Endothelial cell (EC) growth on uncoated and PVDF-HFP–coated PtCr stents (3.0 mm×16 mm, ELEMENT) was evaluated using human coronary artery ECs (HCAECs; Cell Applications Inc, San Diego, CA), as previously described,11 and is described in detail in the online-only Data Supplement.

Techniques: Immunohistochemical staining, Staining, Expressing, Comparison, Control

Journal: bioRxiv

Article Title: miR-10b Deficiency Affords Atherosclerosis Resistance

doi: 10.1101/248641

Figure Lengend Snippet: A. Nucleotide sequences of miR-10b and those of its target in 3’ UTR in LTBP1. B. Western blotting for LTBP1 and β-tubulin (TUBB) using various human ECs that showed Type-I phenotypes (HUVEC, HAEC, HCAEC, HMVEC and ESdEC[P6]) and those with Type-II phenotypes (iPS(BJ)EC, iPS(HU)EC, ESdEC[P0] and ESdEC[P1]) as reported previously 1 . C . Type-II ECs that were transfected with an empty vector (CMV-vector (+), mock) or an miR-10b expression vector (CMV-vector (+), miR-10b), and Type-II ECs without transfection (CMV-vector (-)) were subjected to Western blotting for using an anti-LTBP1 body or an anti-β-tubulin (TUBB) antibody. D . Western blotting for LTBP1 and β-tubulin (TUBB) proteins using Type-I ECs that were transfected with a control HIV vector (control) or an miR-10b inhibitor-expressing HIV vector (miR-10bi) together with Western blotting using Type-II ECs without transfection were shown as indicated. E. Proliferation indexes of SMCs that were co-cultured with Type-II ECs in the absence or the presence of increasing concentrations of LY2157299, a TGF-β signaling inhibitor, as indicated. N=3. F and G . SMCs were co-cultured with Type-I or Type-II ECs and the percentages of phosphorylated SMAD2/3-positive cells in were calculated by flow cytometry (F) and nuclear localization of SMAD3 in PKH-26 (red)-stained SMCs was estimated by immunostaining studies with an anti-smad3 antibody (green) along with nuclear counterstaining with DAPI (blue) (G). Full-length blots are presented in Supplementary information. Abbreviations: ESdEC[P6], human ES cell-derived ECs at passage 6; ESdEC[P0], human ES cell-derived ECs at passage 0; ESdEC[P1], human ES cell-derived ECs at passage 1.

Article Snippet: Human umbilical vein endothelial cells (HUVEC), human aortic endothelial cells (HAEC), human microvascular endothelial cells (HMVEC) and human coronary arterial endothelial cells (HCAEC) were purchased from Dainippon Sumitomo Pharma Co., Ltd. (Osaka Japan).

Techniques: Western Blot, Transfection, Plasmid Preparation, Expressing, Cell Culture, Flow Cytometry, Staining, Immunostaining, Derivative Assay

Journal: Metabolomics

Article Title: Metabolic fingerprints of human primary endothelial and fibroblast cells

doi: 10.1007/s11306-016-1024-7

Figure Lengend Snippet: Different phenotypes of human primary endothelial and fibroblast cells. Micrographs show normal, healthy endothelial cells isolated from coronary arteries, umbilical vein and fibroblasts from the lungs. HCAEC human coronary artery endothelial cells, HUVEC human umbilical vein endothelial cells and NHLF normal human lung fibroblasts

Article Snippet: HCAEC human coronary artery endothelial cells, HUVEC human umbilical vein endothelial cells and NHLF normal human lung fibroblasts Fig. 2 Optimization of Biolog media, redox dye mixes and cell density, for HCAEC human coronary artery endothelial cells and NHLF normal human lung fibroblasts.

Techniques: Isolation

Journal: Metabolomics

Article Title: Metabolic fingerprints of human primary endothelial and fibroblast cells

doi: 10.1007/s11306-016-1024-7

Figure Lengend Snippet: Optimization of Biolog media, redox dye mixes and cell density, for HCAEC human coronary artery endothelial cells and NHLF normal human lung fibroblasts. The OmniLog reading results, as area under curve , of the separate wells, are presented in green

Article Snippet: HCAEC human coronary artery endothelial cells, HUVEC human umbilical vein endothelial cells and NHLF normal human lung fibroblasts Fig. 2 Optimization of Biolog media, redox dye mixes and cell density, for HCAEC human coronary artery endothelial cells and NHLF normal human lung fibroblasts.

Techniques:

Journal: Metabolomics

Article Title: Metabolic fingerprints of human primary endothelial and fibroblast cells

doi: 10.1007/s11306-016-1024-7

Figure Lengend Snippet: HCAEC, HUVEC and NHLF assayed in PM-M1 to PM-M4. The columns show the endpoint of a 24 h incubation of a representative replicate of three independent experiments performed. Positive wells with glucose utilization are indicated with black boxes , while negative, background wells are shown with blue boxes . Exclusive results obtained for HCAEC are shown in green boxes and for NHLF in yellow boxes . HCAEC human coronary artery endothelial cells, HUVEC human umbilical vein endothelial cells and NHLF normal human lung fibroblasts

Article Snippet: HCAEC human coronary artery endothelial cells, HUVEC human umbilical vein endothelial cells and NHLF normal human lung fibroblasts Fig. 2 Optimization of Biolog media, redox dye mixes and cell density, for HCAEC human coronary artery endothelial cells and NHLF normal human lung fibroblasts.

Techniques: Incubation

Journal: Metabolomics

Article Title: Metabolic fingerprints of human primary endothelial and fibroblast cells

doi: 10.1007/s11306-016-1024-7

Figure Lengend Snippet: Comparison of substrate utilization in all three cell types are shown on plates PM-M1 and PM-M2, PM-M3 and PM-M4. Cells were assayed according to the standard protocol and data collected after 24 h using the OmniLog and PM software, with subtraction of the background. Average height (mOD) of tetrazolium reduction was measured in triplicate. HCAEC human coronary artery endothelial cells, HUVEC human umbilical vein endothelial cells and NHLF normal human lung fibroblasts

Article Snippet: HCAEC human coronary artery endothelial cells, HUVEC human umbilical vein endothelial cells and NHLF normal human lung fibroblasts Fig. 2 Optimization of Biolog media, redox dye mixes and cell density, for HCAEC human coronary artery endothelial cells and NHLF normal human lung fibroblasts.

Techniques: Comparison, Software

Journal: Metabolomics

Article Title: Metabolic fingerprints of human primary endothelial and fibroblast cells

doi: 10.1007/s11306-016-1024-7

Figure Lengend Snippet: Unique substrates metabolized exclusively by HCAEC, HUVEC and NHLF, as well as overlapping substrates, are represented by the Venn diagram. HCAEC human coronary artery endothelial cells, HUVEC human umbilical vein endothelial cells and NHLF normal human lung fibroblasts

Article Snippet: HCAEC human coronary artery endothelial cells, HUVEC human umbilical vein endothelial cells and NHLF normal human lung fibroblasts Fig. 2 Optimization of Biolog media, redox dye mixes and cell density, for HCAEC human coronary artery endothelial cells and NHLF normal human lung fibroblasts.

Techniques: