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Image Search Results
Journal: Nucleic Acids Research
Article Title: BORIS/CTCFL-mediated transcriptional regulation of the hTERT telomerase gene in testicular and ovarian tumor cells
doi: 10.1093/nar/gkq827
Figure Lengend Snippet: pBIG-BORIS inducible expression vector or the pBIG-empty vector were transfected into NHBE cell line (Normal Human Bronchial Epithelial). BORIS expression was induced with 1/16 µg/ml (0.0625 µg/ml) or 1 µg/ml of doxycycline and maintained in culture. RNAs from cells were extracted 4, 7 and 10 days after transfection. Parental cells and cells transfected with empty vector died after 7 days. The transcriptional expression of BORIS and hTERT were measured by quantitative RT–PCR and normalized on GAPDH levels.
Article Snippet: The
Techniques: Expressing, Plasmid Preparation, Transfection, Quantitative RT-PCR