Journal: eLife

Article Title: High-molecular-weight polymers from dietary fiber drive aggregation of particulates in the murine small intestine

doi: 10.7554/eLife.40387

Figure Lengend Snippet: The 1-µm-diameter PEG-coated particles form aggregates in fluid collected from the upper ( A-C ) and lower ( D ) SI in ~10 min. ( A and D ) Maximum z-projections of 10 optical slices taken on a confocal microscope. ( B and C ) 3D renderings of aggregates found in panel A. ( E ) Maximum z-projection of the same particles in Hanks' balanced salt solution (HBSS). Scale bars are 10 µm in 2D images and 2 µm in 3D images. ( F and H ) Volume-weighted empirical cumulative distribution functions (ECDFs) comparing aggregation of the particles in pooled samples from the upper ( F ) and lower ( H ) SI of three separate groups of male chow-fed mice (each group consisted of three mice) and a control (particles suspended in HBSS). The vertical axis is the cumulative volume fraction of the total number of particles in solution in an aggregate of a given size. The horizontal axis (aggregate size) is given as the number of particles per aggregate (N). ( G and I ) Box plots depicting the 95% empirical bootstrap CI of the volume-weighted average aggregate size (given in number of particles per aggregate, N) in samples from the upper ( G ) and lower ( I ) SI (the samples are the same as those from panels F and H). The line bisecting the box is the 50 th percentile, the upper and lower edges of the box are the 25 th and 75 th percentile respectively, and the whiskers are the 2.5 th and 97.5 th percentiles. USI = upper SI; LSI = lower SI. See Materials and methods for bootstrapping procedure.

Article Snippet: In PEG solution experiments and serial dilution experiments, HBSS (without calcium, magnesium, phenol red; GE Healthcare Life Sciences) was used to dilute.

Techniques: Microscopy

Journal: eLife

Article Title: High-molecular-weight polymers from dietary fiber drive aggregation of particulates in the murine small intestine

doi: 10.7554/eLife.40387

Figure Lengend Snippet: ( A ) Volume-weighted average sizes for serial dilutions of 1 MDa PEG solutions in a phosphate buffered saline solution with p H = 6.0 ± 0.1 (labeled pH = 6.0) and in Hanks' balanced salt solution (HBSS) with p H = 7.6 ± 0.1 (same data from ). Volume-weighted average sizes are plotted on the vertical axis in terms of number of particles per aggregate (N) against polymer mass concentration (c p ) in mg/mL. The vertical error bars are 95% empirical bootstrap CI (see Materials and methods for bootstrapping procedure).

Article Snippet: In PEG solution experiments and serial dilution experiments, HBSS (without calcium, magnesium, phenol red; GE Healthcare Life Sciences) was used to dilute.

Techniques: Labeling, Concentration Assay

Journal: eLife

Article Title: High-molecular-weight polymers from dietary fiber drive aggregation of particulates in the murine small intestine

doi: 10.7554/eLife.40387

Figure Lengend Snippet: Volume-weighted average sizes for serial dilutions of 1 MDa PEG solutions in Hanks' balanced salt solution (HBSS). Volume-weighted average sizes are plotted on the vertical axis in units of number of particles per aggregate ( N ) against the ‘normalized polymer concentration.’ The normalized polymer concentration is the polymer mass concentration (c p ) in mg/mL divided by the overlap concentration of each polymer solution (c p * ) in mg/mL. The overlap concentrations for PEG 1 MDa, 100 kDa, and 3350 Da are c p * =1.6, 8.6, and 52.6 mg/mL, respectively. The vertical error bars are 95% empirical bootstrap CI (see Materials and methods for bootstrapping procedure).

Article Snippet: In PEG solution experiments and serial dilution experiments, HBSS (without calcium, magnesium, phenol red; GE Healthcare Life Sciences) was used to dilute.

Techniques: Concentration Assay