Journal: Data in Brief

Article Title: The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cells

doi: 10.1016/j.dib.2016.03.040

Figure Lengend Snippet: (A) Immunofluorescence image of p65 siRNA knock down effect after reverse transfection for 7 days ( n =6 array spots) in hBMSCs. (B) Quantitative analysis for p65 positive cells ratio and cell numbers of (A). The figure images were acquired by an ImageXpress Ultra point scanning confocal microscope with an anti-p65 antibody. Blue: Draq5 for the nucleus, Green: fluorescence-labeled antibody to p65 protein. Scramble siRNA was used control. Each error bar represents the mean±SD. (** p <0.01: statistical significance comparison of scramble siRNA). Scale bar=100 µm.

Article Snippet: hBMSCs, human bone marrow stromal cells, (Lonza, Basel, Switzerland) were cultured in MSC basal medium (MSCBM; Lonza), supplemented with BMSC growth medium (MSCGM), an hBMSCs SingleQuots kit (Lonza), 5% fetal bovine serum, 1% l -glutamine, and 0.1% GA-1000.

Techniques: Immunofluorescence, Knockdown, Transfection, Microscopy, Fluorescence, Labeling, Control, Comparison

Journal: Data in Brief

Article Title: The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cells

doi: 10.1016/j.dib.2016.03.040

Figure Lengend Snippet: The examination of siRNA activity from cell-defined microarray by storage period in hBMSCs. (A) The immunofluorescence image of p65 siRNA knock down effect after cell defined siRNA microarray stored for 10 days and 2 months at 4 °C ( n =9 array spots, scale bar=100 µm). (B) Quantitative analysis for p65 positive cells ratio of (A). The figure images were acquired by an ImageXpress Ultra point scanning confocal microscope with an anti-p65 antibody. Blue: Draq5 for the nucleus, Green: fluorescence-labeled antibody to p65 protein. Scramble siRNA was used control. Each error bar represents the mean±SD. (*** p <0.001: statistical significance comparison of scramble siRNA, **p<0.01: statistical significance in comparison between the p65siRNA condition of 10 days and 2 months).

Article Snippet: hBMSCs, human bone marrow stromal cells, (Lonza, Basel, Switzerland) were cultured in MSC basal medium (MSCBM; Lonza), supplemented with BMSC growth medium (MSCGM), an hBMSCs SingleQuots kit (Lonza), 5% fetal bovine serum, 1% l -glutamine, and 0.1% GA-1000.

Techniques: Activity Assay, Microarray, Immunofluorescence, Knockdown, Microscopy, Fluorescence, Labeling, Control, Comparison

Journal: Data in Brief

Article Title: The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cells

doi: 10.1016/j.dib.2016.03.040

Figure Lengend Snippet:

Article Snippet: hBMSCs, human bone marrow stromal cells, (Lonza, Basel, Switzerland) were cultured in MSC basal medium (MSCBM; Lonza), supplemented with BMSC growth medium (MSCGM), an hBMSCs SingleQuots kit (Lonza), 5% fetal bovine serum, 1% l -glutamine, and 0.1% GA-1000.

Techniques: Microarray, Microscopy, Cell Culture, Knockdown, Incubation, Cell Attachment Assay, Activity Assay, Transfection

Journal:

Article Title: Human bone marrow stem cell-encapsulating calcium phosphate scaffolds for bone repair

doi: 10.1016/j.actbio.2010.04.029

Figure Lengend Snippet: Mechanical properties of CPC constructs. Constructs contain 50% v/v alginate beads and 130,000 hBMSCs.

Article Snippet: 2.2. hBMSC culture and encapsulation hBMSCs (Poietics, Lonza, Allendale, NJ) were cultured following established protocols [ 34 ] at 37 °C with 5% CO 2 in low glucose Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, Carlsbad, CA).

Techniques: Construct

Journal:

Article Title: Human bone marrow stem cell-encapsulating calcium phosphate scaffolds for bone repair

doi: 10.1016/j.actbio.2010.04.029

Figure Lengend Snippet: hBMSCs encapsulated in alginate on day 1: (A) live cells in alginate alone; (B) live cells in CPC control; (C) live cells in CPC–chitosan; (D) live cells in CPC–chitosan–fiber. hBMSCs encapsulated in alginate on day 21: (E) live cells in alginate alone; (F) live cells in CPC control; (G) live cells in CPC–chitosan; (H) live cells in CPC–chitosan–fiber.

Article Snippet: 2.2. hBMSC culture and encapsulation hBMSCs (Poietics, Lonza, Allendale, NJ) were cultured following established protocols [ 34 ] at 37 °C with 5% CO 2 in low glucose Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, Carlsbad, CA).

Techniques: Control

Journal:

Article Title: Human bone marrow stem cell-encapsulating calcium phosphate scaffolds for bone repair

doi: 10.1016/j.actbio.2010.04.029

Figure Lengend Snippet: Live/dead results of encapsulated hBMSCs inside alginate hydrogel beads, CPC, CPC–chitosan and CPC–chitosan–fiber constructs (means ± SD, n = 6). (A) Percentage of live cells on days 1, 7, 14 and 21. (B) Live cell density inside the four different constructs on days 1, 7, 14 and 21. Dissimilar letters in the plot indicate values that are significantly different (Tukey’s multiple comparison test, family confidence coefficient 0.95).

Article Snippet: 2.2. hBMSC culture and encapsulation hBMSCs (Poietics, Lonza, Allendale, NJ) were cultured following established protocols [ 34 ] at 37 °C with 5% CO 2 in low glucose Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, Carlsbad, CA).

Techniques: Construct, Comparison

Journal:

Article Title: Human bone marrow stem cell-encapsulating calcium phosphate scaffolds for bone repair

doi: 10.1016/j.actbio.2010.04.029

Figure Lengend Snippet: Wst-1 assay results for encapsulated hBMSCs inside alginate hydrogel beads, CPC, CPC–chitosan and CPC–chitosan–fiber constructs (means ± SD, n = 6). The viability of hBMSCs was quantified by recording the absorbance at 450 nm as an indication of dehydrogenase activity. Dissimilar letters in the plot indicate values that are significantly different (Tukey’s multiple comparison test, family confidence coefficient 0.95).

Article Snippet: 2.2. hBMSC culture and encapsulation hBMSCs (Poietics, Lonza, Allendale, NJ) were cultured following established protocols [ 34 ] at 37 °C with 5% CO 2 in low glucose Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, Carlsbad, CA).

Techniques: WST-1 Assay, Construct, Activity Assay, Comparison

Journal:

Article Title: Human bone marrow stem cell-encapsulating calcium phosphate scaffolds for bone repair

doi: 10.1016/j.actbio.2010.04.029

Figure Lengend Snippet: ALP activity of encapsulated hBMSCs inside alginate hydrogel beads, and inside CPC, CPC–chitosan and CPC–chitosan–fiber constructs (means ± SD, n = 6). The ALP value was normalized to the DNA concentration, with units of mM pNpp min−1/µg DNA. Dissimilar letters in the plot indicate values that are significantly different (Tukey’s multiple comparison test, family confidence coefficient 0.95).

Article Snippet: 2.2. hBMSC culture and encapsulation hBMSCs (Poietics, Lonza, Allendale, NJ) were cultured following established protocols [ 34 ] at 37 °C with 5% CO 2 in low glucose Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, Carlsbad, CA).

Techniques: Activity Assay, Construct, Concentration Assay, Comparison

Journal:

Article Title: Human bone marrow stem cell-encapsulating calcium phosphate scaffolds for bone repair

doi: 10.1016/j.actbio.2010.04.029

Figure Lengend Snippet: Mineralization by hBMSCs in hydrogel beads in the CPC–chitosan–fiber construct on (A) day 7 and (B) day 14, cultured in osteogenic medium. The live cell culture was stained with xylenol orange, which stains mineral a red color (means ± SD, n = 6). (C) hBMSC mineralization area fraction, which is the area of stained mineralization divided by the total area of the field of view of the image. This was done for the encapsulated hBMSCs in alginate hydrogel beads, in CPC, in CPC–chitosan and in CPC–chitosan–fiber constructs. Dissimilar letters in (C) indicate values that are significantly different (Tukey’s multiple comparison test, family confidence coefficient 0.95).

Article Snippet: 2.2. hBMSC culture and encapsulation hBMSCs (Poietics, Lonza, Allendale, NJ) were cultured following established protocols [ 34 ] at 37 °C with 5% CO 2 in low glucose Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, Carlsbad, CA).

Techniques: Construct, Cell Culture, Staining, Comparison

Journal:

Article Title: Human bone marrow stem cell-encapsulating calcium phosphate scaffolds for bone repair

doi: 10.1016/j.actbio.2010.04.029

Figure Lengend Snippet: SEM showing significant mineral formation via hBMSCs. (A) Mineral synthesized by hBMSCs encapsulated in alginate hydrogel beads on day 21 and (B) mineral synthesized by hBMSCs encapsulated in beads in the CPC–chitosan–fiber construct on day 21.

Article Snippet: 2.2. hBMSC culture and encapsulation hBMSCs (Poietics, Lonza, Allendale, NJ) were cultured following established protocols [ 34 ] at 37 °C with 5% CO 2 in low glucose Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, Carlsbad, CA).

Techniques: Synthesized, Construct