Journal: Kidney international

Article Title: Coexpression of CD9 augments the ability of membrane-bound heparin-binding epidermal growth factor-like growth factor (proHB-EGF) to preserve renal epithelial cell viability.

doi: 10.1046/j.1523-1755.1999.00259.x

Figure Lengend Snippet: Fig. 1. Expression of CD9 and HB-EGF mRNA in transfected NRK-52E cells trans- fected with rat proHB-EGF cDNA. Cells were transfected with CD91 proHB-EGF cDNA (NRKboth) (lane A), proHB-EGF (NRKproHB-EGF) (lane B), empty vector (NRKvector) (lane C) or CD9 (NRKCD9) (lane D) and stable transfectants were selected by neomycin resistance. Total RNA was hybrid- ized with rat CD9 or rat HB-EGF cDNA as indicated. The housekeeping gene GAPDH is indicated for comparison.

Article Snippet: Cells were exposed to 0.5 was obtained from Pharmigen (San Diego, CA, USA). mm H2O2 for the indicated times and were collected Anti-HB-EGF antibodies were from Santa Cruz Bio- by scraping cells into media in which they had been technology (Santa Cruz, CA, USA); monoclonal antirat incubated.

Techniques: Expressing, Transfection, Plasmid Preparation, Comparison

Journal: Kidney international

Article Title: Coexpression of CD9 augments the ability of membrane-bound heparin-binding epidermal growth factor-like growth factor (proHB-EGF) to preserve renal epithelial cell viability.

doi: 10.1046/j.1523-1755.1999.00259.x

Figure Lengend Snippet: Fig. 2. Immunoreactive CD9 and HB-EGF expression in transfected cells. Expression of CD9 (A and C) and HB-EGF (B and D) were determined in (NRKvector) (A and B) or (NRKboth) (C and D).

Article Snippet: Cells were exposed to 0.5 was obtained from Pharmigen (San Diego, CA, USA). mm H2O2 for the indicated times and were collected Anti-HB-EGF antibodies were from Santa Cruz Bio- by scraping cells into media in which they had been technology (Santa Cruz, CA, USA); monoclonal antirat incubated.

Techniques: Expressing, Transfection

Journal: Kidney international

Article Title: Coexpression of CD9 augments the ability of membrane-bound heparin-binding epidermal growth factor-like growth factor (proHB-EGF) to preserve renal epithelial cell viability.

doi: 10.1046/j.1523-1755.1999.00259.x

Figure Lengend Snippet: Fig. 5. Effect of CD9 1 proHB-EGF to prevent H2O2-induced apoptosis in renal epithelial cells. (A) Cells were treated with 0.5 mM H2O2 for 30 hours and stained with bisbenzamide H33258 as described in the Methods section. Representative fields of NRKvector (left) indicate characteristic morphologic alterations and apoptotic bodies compared to relative structural preservation in the majority of NRKboth (right). (B) DNA laddering in response to hydrogen peroxide. Subconfluent cells were made quiescent by incubation for two days in DMEM supplemented with 0.4% FCS and were then exposed to 0.5 mM H2O2 for the indicated times. Both the cell monolayer and floating cells were collected and analyzed.

Article Snippet: Cells were exposed to 0.5 was obtained from Pharmigen (San Diego, CA, USA). mm H2O2 for the indicated times and were collected Anti-HB-EGF antibodies were from Santa Cruz Bio- by scraping cells into media in which they had been technology (Santa Cruz, CA, USA); monoclonal antirat incubated.

Techniques: Staining, Preserving, DNA Laddering, Incubation

Journal: Kidney international

Article Title: Coexpression of CD9 augments the ability of membrane-bound heparin-binding epidermal growth factor-like growth factor (proHB-EGF) to preserve renal epithelial cell viability.

doi: 10.1046/j.1523-1755.1999.00259.x

Figure Lengend Snippet: Fig. 8. Expression of immunoreactive b1 in- tegrin. Immunofluorescent detection of b1 in- tegrin with a rat anti b1 integrin antibody (CD29) was performed as described in the “Ex- perimental Methods” section. NRKvector (A), NRKCD9 (B), NRKproHB-EGF (C), NRKboth (D).

Article Snippet: Cells were exposed to 0.5 was obtained from Pharmigen (San Diego, CA, USA). mm H2O2 for the indicated times and were collected Anti-HB-EGF antibodies were from Santa Cruz Bio- by scraping cells into media in which they had been technology (Santa Cruz, CA, USA); monoclonal antirat incubated.

Techniques: Expressing

Journal: Nature neuroscience

Article Title: A glycolytic shift in Schwann cells supports injured axons

doi: 10.1038/s41593-020-0689-4

Figure Lengend Snippet: a, Top: Scheme of metabolomic analysis using extracts from nerve segments. Bottom: Concentrations of key energy metabolism intermediates in control and axotomized nerve segments from C57Bl/6J mice (F6P/G6P: fructose-6-phosphate/glucose-6-phosphate. FBP: fructose-1,6-bisphosphate. GI-OH3P: Glyceraldehyde-3-phosphate. 2PG/3PG: 2-phosphoglycerate/3-phosphoglycerate. LACT: lactate) (Error bars represent s.e.m. n=5 mice per condition and metabolite). b, Top: Scheme of extracellular flux analysis of SCs purified from C57Bl/6J mouse nerves. Bottom: Box and whiskers plot (maximum, 25th percentile, median, 75th percentile, minimum) shows glycolytic activity parameters as assessed by extracellular acidification rate (ECAR) measurements in control mouse SCs and cells with Nrg1-induced ErbB2 activation (n=9 well preparations per condition, *P=0.0080, **P=0.0464, ***P<0.0001). c, Western blot analysis (cropped blot images) of control- and Nrg1-treated C57Bl/6J mouse SCs probed with the indicated antibodies. (n=6 independent pair preparations (2 separate dishes) for PFKFB3, and n=3 independent pair preparations (2 separate dishes) for LDHA quantification). d, Intracellular and extracellular (supernatant) lactate concentrations from control and Nrg1-treated mouse SC preparations normalized to cell number and cellular protein. Note decreased intracellular and increased extracellular lactate levels in SCs treated with Nrg1 for 24h, indicating greatly increased lactate extrusion (Error bars represent s.e.m. n=3 well preparations from 3 independent experiments per condition).

Article Snippet: SCs were subsequently control-treated or treated with 200 ng/ml recombinant Nrg1 (R&D Systems, 396-HB-050) for 24h, collected in RIPA buffer containing phosphatase and protease inhibitors, and then processed for protein analysis and western blotting using standard procedures.

Techniques: Control, Purification, Activity Assay, Activation Assay, Western Blot

Journal:

Article Title: The Role of Ultraviolet Irradiation and Heparin-Binding Epidermal Growth Factor-Like Growth Factor in the Pathogenesis of Pterygium

doi:

Figure Lengend Snippet: Expression of HB-EGF in pterygia. Pterygium tissue (A through F), normal limbus (G), and normal conjuntiva (H) were analyzed by immunohistochemistry to determine the expression of HB-EGF. Sections A, C, E, G, and H included the anti-HB-EGF antibody and sections B, D, and F were incubated without primary antibody and served as controls. All tissue sections were counterstained with hematoxylin. These data are representative of all pterygia examined. Original magnifications, ×600.

Article Snippet: 22 After blocking specimens in swine serum, a goat anti-human HB-EGF antibody (R&D Systems) was added to each section overnight at 4°C.

Techniques: Expressing, Immunohistochemistry, Incubation

Journal:

Article Title: The Role of Ultraviolet Irradiation and Heparin-Binding Epidermal Growth Factor-Like Growth Factor in the Pathogenesis of Pterygium

doi:

Figure Lengend Snippet: Genes Up-Regulated in PECs 12 Hours after UVB Exposure Compared to Nonirradiated PECs

Article Snippet: 22 After blocking specimens in swine serum, a goat anti-human HB-EGF antibody (R&D Systems) was added to each section overnight at 4°C.

Techniques:

Journal:

Article Title: The Role of Ultraviolet Irradiation and Heparin-Binding Epidermal Growth Factor-Like Growth Factor in the Pathogenesis of Pterygium

doi:

Figure Lengend Snippet: RT-PCR analysis for HB-EGF (750 bp) mRNA in UVB stimulated PEC (A) and LEC (C). B and D represent RT-PCR for GAPDH (240 bp) in PEC and LEC, respectively. Equal amounts of total RNA were reverse-transcribed from both cell types in all lanes. Time points at 0, 2, 6, 12, and 24 hours after irradiation are represented by lanes 2 to 6, respectively. When no reverse-transcriptase enzyme and no gene-specific primers were included, no PCR product formed (lanes 7 and 8, respectively). A 100-bp ladder (Promega) was run in parallel (lane 1). These results are representative of triplicate experiments in three different cell lines.

Article Snippet: 22 After blocking specimens in swine serum, a goat anti-human HB-EGF antibody (R&D Systems) was added to each section overnight at 4°C.

Techniques: Reverse Transcription Polymerase Chain Reaction, Reverse Transcription, Irradiation

Journal:

Article Title: The Role of Ultraviolet Irradiation and Heparin-Binding Epidermal Growth Factor-Like Growth Factor in the Pathogenesis of Pterygium

doi:

Figure Lengend Snippet: Genes Up-Regulated in PECs 6 Hours after UVB Exposure Compared to Nonirradiated PECs

Article Snippet: 22 After blocking specimens in swine serum, a goat anti-human HB-EGF antibody (R&D Systems) was added to each section overnight at 4°C.

Techniques: