Journal: International Journal of Microbiology

Article Title: Effect of Citrus Byproducts on Survival of O157:H7 and Non-O157 Escherichia coli Serogroups within In Vitro Bovine Ruminal Microbial Fermentations

doi: 10.1155/2013/398320

Figure Lengend Snippet: Least squares means for growth of STEC O26:H11, O103:H8, O111:H8, O145:H28, O157:H7, and O157:H7 Δ stx 1 stx 2 within bovine mixed rumen microorganisms fluid medium, supplemented with 0%, 5%, 10%, and 20% powdered citrus by-products (CBPs).

Article Snippet: Six strains of E. coli included in this study were purchased from the American Type Culture Collection (ATCC): O157:H7 (ATCC 43895), O103:H8 (ATCC 23982), O145:H28 (BAA-2129), O26:H11 (BAA-1653), O111:H8 (BAA-179), and O157:H7 Δ stx 1 stx 2 (ATCC 43888).

Techniques:

Journal: International Journal of Microbiology

Article Title: Effect of Citrus Byproducts on Survival of O157:H7 and Non-O157 Escherichia coli Serogroups within In Vitro Bovine Ruminal Microbial Fermentations

doi: 10.1155/2013/398320

Figure Lengend Snippet: Least squares means for growth of STEC O26:H11, O103:H8, O111:H8, O145:H28, O157:H7, and O157:H7 Δ stx 1 stx 2 within bovine mixed rumen microorganisms fluid medium, supplemented with 0%, 5%, 10%, and 20% pelleted citrus by-products (CBPs).

Article Snippet: Six strains of E. coli included in this study were purchased from the American Type Culture Collection (ATCC): O157:H7 (ATCC 43895), O103:H8 (ATCC 23982), O145:H28 (BAA-2129), O26:H11 (BAA-1653), O111:H8 (BAA-179), and O157:H7 Δ stx 1 stx 2 (ATCC 43888).

Techniques:

Journal: Journal of Translational Medicine

Article Title: ADSC-enriched adipose extract alleviates cartilage fibrosis in temporomandibular joint osteoarthritis by inhibiting chondrocyte senescence

doi: 10.1186/s12967-025-07108-8

Figure Lengend Snippet: Therapeutic efficacy of ARDE in TMJOA model animals. A , B Changes in HWT and PMMO. C Representative HE-stained images of condylar cartilage. The fibrocartilage areas are marked by dashed red lines. D Representative Safranin O-stained images of condylar cartilage. E Modified Mankin OA scoring. F Changes in fibrocartilage layer thickness. G Representative images of ACTA2, COL-I, and COL-III immunofluorescence staining in the condylar cartilage. H – J Quantification of the mean fluorescence intensity in Figure G. K Representative images of immunofluorescence staining for MMP-13, IL-1β, and TNF-α in the condylar cartilage. L – N Quantification of the mean fluorescence intensity in Figure K. All the data are expressed as the means ± SDs. The test of significance is Student’s t test ( n = 5, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). Scale bar: 100 μm

Article Snippet: Immunofluorescence staining: After washing the paraffin sections with PBS, goat serum blocking solution (ZSGB-Biotech, Beijing, China, ZLI-9056) was applied and incubated for 30 min. Primary antibodies against MMP-13 (1:500, Novus Biologicals, Littleton, CO, USA, NBP1-45723), IL-1β (1:500, Proteintech, Wuhan, China, 66737-1-Ig), TNF-α (1:500, Novus Biologicals, Littleton, CO, USA, NBP2-34301), ACTA2 (1:200, Abcam, Cambridge, MA, USA, ab7817), collagen I (1:500, Proteintech, Wuhan, China, 66761-1-Ig), collagen III (1:500, Proteintech, Wuhan, China, 68320-1-Ig) and p53 (1:500, Proteintech, Wuhan, China, 60283-2-Ig) were diluted according to the manufacturer’s instructions and incubated overnight at 4°C.

Techniques: Drug discovery, Staining, Modification, Immunofluorescence, Fluorescence

Journal: Journal of Translational Medicine

Article Title: ADSC-enriched adipose extract alleviates cartilage fibrosis in temporomandibular joint osteoarthritis by inhibiting chondrocyte senescence

doi: 10.1186/s12967-025-07108-8

Figure Lengend Snippet: ADSC coculture alleviates senescence in the TMJOA cell model. A GSEA of senescence-related gene sets between IL-1β-induced chondrocytes and normal chondrocytes. B GSEA of senescence-related gene sets between TNF-α-induced chondrocytes and normal chondrocytes. The results revealed increasing trends in senescence-related gene sets in inflammatory factor-induced chondrocytes. C Representative images of the cell cycle distribution. D Proportions of viable cells in the G1, S, and G2 phases among the groups ( n = 3, * p < 0.05, *** p < 0.001, **** p < 0.0001). E Representative β-galactosidase staining images. F Percentage of β-galactosidase-positive cells ( n = 5, ** p < 0.01). All the data are expressed as the means ± SDs. The test of significance is one-way ANOVA. Scale bar: 100 μm

Article Snippet: Immunofluorescence staining: After washing the paraffin sections with PBS, goat serum blocking solution (ZSGB-Biotech, Beijing, China, ZLI-9056) was applied and incubated for 30 min. Primary antibodies against MMP-13 (1:500, Novus Biologicals, Littleton, CO, USA, NBP1-45723), IL-1β (1:500, Proteintech, Wuhan, China, 66737-1-Ig), TNF-α (1:500, Novus Biologicals, Littleton, CO, USA, NBP2-34301), ACTA2 (1:200, Abcam, Cambridge, MA, USA, ab7817), collagen I (1:500, Proteintech, Wuhan, China, 66761-1-Ig), collagen III (1:500, Proteintech, Wuhan, China, 68320-1-Ig) and p53 (1:500, Proteintech, Wuhan, China, 60283-2-Ig) were diluted according to the manufacturer’s instructions and incubated overnight at 4°C.

Techniques: Staining

Journal: Vaccines

Article Title: Evaluation of Immunogenicity and Efficacy of Fasciola hepatica Tetraspanin 2 (TSP2) Fused to E. coli Heat-Labile Enterotoxin B Subunit LTB Adjuvant Following Intranasal Vaccination of Cattle

doi: 10.3390/vaccines9111213

Figure Lengend Snippet: Characterisation of LTB oligomerisation. Both proteins rLTB-FhTSP2 ( a , c ) and rLTB ( b , d ) were loaded at 6 µg and subject to boiling at 95 °C for 10 min. Boiled (lane 1) and non-boiled (lane 2) proteins were analysied by SDS-PAGE and visualized by Coomassie staining ( a , b ) and Western blotting ( c , d ) using anti-His tag antibodies.

Article Snippet: After washing five times with 1× PBS-T, plates were incubated for 1 h at RT with 100 μL/well of mouse anti-His tag HRP conjugate antibodies (R&D Systems) diluted 1:10,000 in blocking buffer C. Plates were washed five times with 1× PBS-T with the reactions developed and stopped and the plate OD read as described above.

Techniques: SDS Page, Staining, Western Blot

Journal: Vaccines

Article Title: Evaluation of Immunogenicity and Efficacy of Fasciola hepatica Tetraspanin 2 (TSP2) Fused to E. coli Heat-Labile Enterotoxin B Subunit LTB Adjuvant Following Intranasal Vaccination of Cattle

doi: 10.3390/vaccines9111213

Figure Lengend Snippet: Characterisation of pentamer formation of the rLTB-FhTSP2 fusion complex via glycan receptor binding. Plates were coated with 3 μg/mL of GM1 ganglioside and blocked with 3% bovine serum albumin. Potential binding proteins (rEcxB and rLTB-FhTSP2) and negative control (rFhTSP2) were incubated in triplicate at three dilution factors (3, 0.3, and 0.03 μg/mL). Binding was detected by mouse anti-His tag HRP conjugated antibodies. All results are displayed as the mean of the adjusted optical density after subtraction of non-GM1 coated wells.

Article Snippet: After washing five times with 1× PBS-T, plates were incubated for 1 h at RT with 100 μL/well of mouse anti-His tag HRP conjugate antibodies (R&D Systems) diluted 1:10,000 in blocking buffer C. Plates were washed five times with 1× PBS-T with the reactions developed and stopped and the plate OD read as described above.

Techniques: Glycoproteomics, Binding Assay, Negative Control, Incubation

Journal: Vaccines

Article Title: Evaluation of Immunogenicity and Efficacy of Fasciola hepatica Tetraspanin 2 (TSP2) Fused to E. coli Heat-Labile Enterotoxin B Subunit LTB Adjuvant Following Intranasal Vaccination of Cattle

doi: 10.3390/vaccines9111213

Figure Lengend Snippet: Characterisation of the antigenicity of the rLTB-FhTSP2 fusion complex. The reactivity of rFhTSP2, rLTB-FhTSP2, and rLTB was analysed using diluted bovine anti-rFhTSP2 sera (1:100, 1:200, 1:400, and 1:800) and a anti-bovine IgG HRP secondary antibody. Antigens were coated at an equal molar ratio of the FhTSP2 protein sequence.

Article Snippet: After washing five times with 1× PBS-T, plates were incubated for 1 h at RT with 100 μL/well of mouse anti-His tag HRP conjugate antibodies (R&D Systems) diluted 1:10,000 in blocking buffer C. Plates were washed five times with 1× PBS-T with the reactions developed and stopped and the plate OD read as described above.

Techniques: Sequencing

Journal: Vaccines

Article Title: Evaluation of Immunogenicity and Efficacy of Fasciola hepatica Tetraspanin 2 (TSP2) Fused to E. coli Heat-Labile Enterotoxin B Subunit LTB Adjuvant Following Intranasal Vaccination of Cattle

doi: 10.3390/vaccines9111213

Figure Lengend Snippet: Bovine humoral response towards rFhTSP2 over the course of the trial. ( a ) Serum IgG, ( b ) serum IgA, and ( c ) saliva IgA responses from Day 0 (pre-vaccination), Day 42 (post-vaccination), Day 84 (post-challenge), and Day 125 (end of trial) from Group 1 (G1: dark grey) subcutaneously vaccinated with PBS; Group 2 (G2: red) intranasally vaccinated with rLTB-FhTSP2; and Group 3 (G3: blue) subcutaneously vaccinated with rFhTSP2. Each animal within a group is represented by a different shape. The reactivity of 5 μg/mL of rFhTSP2 was analysed using serum (diluted 1:50) or saliva (diluted 1:8) and an anti-bovine IgG HRP secondary antibody diluted 1:5000 or a anti-bovine IgA HRP antibody diluted 1:2000. The mean OD of each group is represented by a solid line. Significant differences ( p < 0.05) of the mean are denoted by asterisks (*).

Article Snippet: After washing five times with 1× PBS-T, plates were incubated for 1 h at RT with 100 μL/well of mouse anti-His tag HRP conjugate antibodies (R&D Systems) diluted 1:10,000 in blocking buffer C. Plates were washed five times with 1× PBS-T with the reactions developed and stopped and the plate OD read as described above.

Techniques: