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China Center for Type Culture Collection
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Sinovac BioTech
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Image Search Results
Journal: Biomolecules
Article Title: TIPE2 Induced the Proliferation, Survival, and Migration of Lung Cancer Cells Through Modulation of Akt/mTOR/NF-κB Signaling Cascade
doi: 10.3390/biom9120836
Figure Lengend Snippet: Knockout of TIPE2 in lung cancer cells and its effect on different cancer hall marks. ( A ) Western blot analysis showing the expression of TIPE2 in NCIH460 lung cancer cells after CRISPR/Cas9 knockout (Left panel); Effect of TIPE2 knockout on the viability of NCIH460 lung cancer cells as analyzed by MTT assay (Right Panel); ( B ) Colony formation assay showing decreased clonogenic potential of lung cancer cells after knockout of TIPE2 (Left panel). Graphical representation of decreased clonogenic potential of TIPE2 knockout cells in terms of survival fraction (Right panel). ( C ) Cell migration was detected with the help of wound healing assay. Images were taken at 10× magnification at 0 and 24 h (Left panel). Graphical representation of the decreased migration potential of TIPE2 knockout cells compared to CRISPR/Cas9 scramble (right panel). ( D ) Effect of TIPE2 knockout on the progression of cell cycle in lung cancer cells analyzed through flow cytometry using PI/RNase solution (Left panel). Graphical representation of the effect of TIPE2 knockout on the cell cycle progression of lung cancer cells (Right panel). Data are represented as mean ± SE, * denotes p value < 0.05.
Article Snippet:
Techniques: Knock-Out, Western Blot, Expressing, CRISPR, MTT Assay, Colony Assay, Migration, Wound Healing Assay, Flow Cytometry
Journal: Theranostics
Article Title: Radiotracer stereochemistry affects substrate affinity and kinetics for improved imaging of system x C - in tumors
doi: 10.7150/thno.63237
Figure Lengend Snippet: Evaluation of [ 18 F]FRPG and [ 18 F]FSPG in tumor-bearing mice. (A) Representative single slice sagittal, coronal and axial (inset) PET/CT images in A549 subcutaneous tumor-bearing mouse 40-60 min after injection of ~3 MBq [ 18 F]FRPG or [ 18 F]FSPG. Imaging with each radiotracer was performed 24 h apart in the same mouse. White arrowheads indicate the tumor margins. (B) Representative PET/CT maximum intensity projections from the same mouse. White arrowheads indicate the tumor margins. SG, salivary glands; P, pancreas; K, kidney; B, bladder. (C) Image-derived quantification of radiotracer retention in matched A549 tumors imaged 24-48 h apart. Data points represent individual animals. *, P < 0.05. (D) Image-derived tumor-to-muscle ratios for [ 18 F]FRPG and [ 18 F]FSPG. Data points represent individual animals. ns, not significant.
Article Snippet: Human cancer H460 and
Techniques: Positron Emission Tomography-Computed Tomography, Injection, Imaging, Derivative Assay