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gw7647  (Tocris)
93
Tocris gw7647
Gw7647, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gw7647+cat/GW+7647/pm29377004-390-41-42
Average 93 stars, based on 1 article reviews
gw7647 - by Bioz Stars, 2026-09
93/100 stars
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gw7647  (Santa Cruz Biotechnology)
91
Santa Cruz Biotechnology gw7647
ZFP961 interacts with PPAR α to block thermogenic activation. a) Co‐immunoprecipitation analysis using cell extracts from HEK293T cells that were co‐transfected with HA‐ZFP961 and Flag‐PPAR α . b) HEK293T cells were co‐transfected with indicated plasmids, and the transcriptional activity of PPAR α was measured with luciferase reporter assay ( n = 4, per group). c) Left panel: co‐immunoprecipitation analysis using cell extracts from HEK293T cells that were co‐transfected with HA‐ZFP961‐WT, HA‐ZFP961‐Mut, or Flag‐PPAR α in the presence or absence of <t>GW7647</t> (1 × 10 −6 m ). Right panel: ZFP961‐Mut and ZFP961 mRNA levels were analyzed by qRT‐PCR ( n = 4, per group). d) The transcriptional activity of PPAR α was measured in HEK293T cells that were co‐transfected with indicated plasmids and treated with or without GW7647 (1 × 10 −6 m ) ( n = 4, per group). e) Mature brown adipocytes were infected with adenovirus expressing ZFP961‐WT, ZFP961‐Mut, or GFP and then treated with or without GW7647 (1 × 10 −6 m ) for 24 h. Gene expression was analyzed ( n = 4, per group). f) Ucp1 protein in mature brown adipocytes generated as in (e). g) Brown preadipocytes infected with lentivirus containing Scr or PPAR α shRNAs were differentiated and then infected with adenovirus expressing ZFP961‐WT or GFP, and gene expression was analyzed ( n = 4, per group). h) Ucp1 protein in mature brown adipocytes generated as in (g). All error bars represent s.e.m. Two‐tailed unpaired Student's t ‐test was performed. * p < 0.05; n.s., not significant.
Gw7647, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gw7647+cat/GW+7647/pmc08805557-184-7-14
Average 91 stars, based on 1 article reviews
gw7647 - by Bioz Stars, 2026-09
91/100 stars
  Buy from Supplier

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ZFP961 interacts with PPAR α to block thermogenic activation. a) Co‐immunoprecipitation analysis using cell extracts from HEK293T cells that were co‐transfected with HA‐ZFP961 and Flag‐PPAR α . b) HEK293T cells were co‐transfected with indicated plasmids, and the transcriptional activity of PPAR α was measured with luciferase reporter assay ( n = 4, per group). c) Left panel: co‐immunoprecipitation analysis using cell extracts from HEK293T cells that were co‐transfected with HA‐ZFP961‐WT, HA‐ZFP961‐Mut, or Flag‐PPAR α in the presence or absence of GW7647 (1 × 10 −6 m ). Right panel: ZFP961‐Mut and ZFP961 mRNA levels were analyzed by qRT‐PCR ( n = 4, per group). d) The transcriptional activity of PPAR α was measured in HEK293T cells that were co‐transfected with indicated plasmids and treated with or without GW7647 (1 × 10 −6 m ) ( n = 4, per group). e) Mature brown adipocytes were infected with adenovirus expressing ZFP961‐WT, ZFP961‐Mut, or GFP and then treated with or without GW7647 (1 × 10 −6 m ) for 24 h. Gene expression was analyzed ( n = 4, per group). f) Ucp1 protein in mature brown adipocytes generated as in (e). g) Brown preadipocytes infected with lentivirus containing Scr or PPAR α shRNAs were differentiated and then infected with adenovirus expressing ZFP961‐WT or GFP, and gene expression was analyzed ( n = 4, per group). h) Ucp1 protein in mature brown adipocytes generated as in (g). All error bars represent s.e.m. Two‐tailed unpaired Student's t ‐test was performed. * p < 0.05; n.s., not significant.

Journal: Advanced Science

Article Title: The KRAB Domain‐Containing Protein ZFP961 Represses Adipose Thermogenesis and Energy Expenditure through Interaction with PPAR α

doi: 10.1002/advs.202102949

Figure Lengend Snippet: ZFP961 interacts with PPAR α to block thermogenic activation. a) Co‐immunoprecipitation analysis using cell extracts from HEK293T cells that were co‐transfected with HA‐ZFP961 and Flag‐PPAR α . b) HEK293T cells were co‐transfected with indicated plasmids, and the transcriptional activity of PPAR α was measured with luciferase reporter assay ( n = 4, per group). c) Left panel: co‐immunoprecipitation analysis using cell extracts from HEK293T cells that were co‐transfected with HA‐ZFP961‐WT, HA‐ZFP961‐Mut, or Flag‐PPAR α in the presence or absence of GW7647 (1 × 10 −6 m ). Right panel: ZFP961‐Mut and ZFP961 mRNA levels were analyzed by qRT‐PCR ( n = 4, per group). d) The transcriptional activity of PPAR α was measured in HEK293T cells that were co‐transfected with indicated plasmids and treated with or without GW7647 (1 × 10 −6 m ) ( n = 4, per group). e) Mature brown adipocytes were infected with adenovirus expressing ZFP961‐WT, ZFP961‐Mut, or GFP and then treated with or without GW7647 (1 × 10 −6 m ) for 24 h. Gene expression was analyzed ( n = 4, per group). f) Ucp1 protein in mature brown adipocytes generated as in (e). g) Brown preadipocytes infected with lentivirus containing Scr or PPAR α shRNAs were differentiated and then infected with adenovirus expressing ZFP961‐WT or GFP, and gene expression was analyzed ( n = 4, per group). h) Ucp1 protein in mature brown adipocytes generated as in (g). All error bars represent s.e.m. Two‐tailed unpaired Student's t ‐test was performed. * p < 0.05; n.s., not significant.

Article Snippet: In some experiments, cells were treated with GW7647 (1 × 10 −6 m ) (Santa Cruz Biotechnology, cat. no. CAS 265129‐71‐3) as indicated.

Techniques: Blocking Assay, Activation Assay, Immunoprecipitation, Transfection, Activity Assay, Luciferase, Reporter Assay, Quantitative RT-PCR, Infection, Expressing, Gene Expression, Generated, Two Tailed Test