gst pull-downs pgex4t-1 Search Results


pgex4t 1  (Cytiva Europe)
96
Cytiva Europe pgex4t 1
Pgex4t 1, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst+pull-downs+pgex4t-1/PGEX-4T-1/pmc07305159-326-16-17
Average 96 stars, based on 1 article reviews
pgex4t 1 - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
pgex4t-1  (Promega)
90
Promega pgex4t-1
Pgex4t 1, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst+pull-downs+pgex4t-1/pgex+vectors/10__1523_slash_jneurosci__2304___08__2008-89-21-22
Average 90 stars, based on 1 article reviews
pgex4t-1 - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
pgex 4t 1  (Addgene inc)
93
Addgene inc pgex 4t 1
Pgex 4t 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst+pull-downs+pgex4t-1/pGEX4T-1-mMcl-1+(Plasmid+%2325717)/10__1074_slash_jbc__ra118__005437-161-4-5
Average 93 stars, based on 1 article reviews
pgex 4t 1 - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier
293  (ATCC)
99
ATCC 293
293, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst+pull-downs+pgex4t-1/293/custom%40crl-1573%4023037926
Average 99 stars, based on 1 article reviews
293 - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
bl21(de3) competent e. coli  (New England Biolabs)
99
New England Biolabs bl21(de3) competent e. coli
Bl21(de3) Competent E. Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst+pull-downs+pgex4t-1/BL21(DE3)+Competent+E%2E+coli/custom%40c2527%4034296248
Average 99 stars, based on 1 article reviews
bl21(de3) competent e. coli - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
plasmids encoding gst-tagged hbc truncations  (Promega)
90
Promega plasmids encoding gst-tagged hbc truncations
Plasmids Encoding Gst Tagged Hbc Truncations, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst+pull-downs+pgex4t-1/plasmids+encoding+gst+tagged+hbc+truncations/10__1128_slash_jvi__03106___14-36-1-27
Average 90 stars, based on 1 article reviews
plasmids encoding gst-tagged hbc truncations - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
gst fusion protein expression  (Danaher Inc)
96
Danaher Inc gst fusion protein expression
Gst Fusion Protein Expression, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst+pull-downs+pgex4t-1/Fusion/10__1074_slash_jbc__m110964200-81-17-12
Average 96 stars, based on 1 article reviews
gst fusion protein expression - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
pgex-4t-1  (Life science market)
90
Life science market pgex-4t-1
Pgex 4t 1, supplied by Life science market, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst+pull-downs+pgex4t-1/pgex+4t+1/ppr0374658-330-11-12
Average 90 stars, based on 1 article reviews
pgex-4t-1 - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
pgex 4t 1 vector  (Addgene inc)
96
Addgene inc pgex 4t 1 vector
CDC73 binds directly to USP37. Schematic diagram of USPs and CDC73 expression vectors ( A ). For GST pull-down analysis, full-length cDNAs encoding USP37, as well as USP12, USP15, USP25 and USP28, were cloned into <t>pGEX-4T-1</t> vector. Full-length wild-type CDC73 was cloned into pGEX-4T-1, and a vector expressing only CDC73 without GST was also constructed. GST/pGEX-4T-1 was used as control. GST pull-down analysis using GST-USP37 and GST-CDC73 ( B , C ). E.coli BL21 was transformed with GST-USP37/pGEX-4T-1 or GST-CDC73/pGEX-4T-1 and induced with 100 uM IPTG for 3 h. Purified GST-USP37 was incubated with a lysate of FLAG-CDC73-transfected cells ( B ), and purified GST-CDC73 was incubated with a lysate of Myc-USP37-transfected cells ( C ). These results show that USP37 is observed to interact strongly with the CDC73 protein. GST, glutathione S-transferase; WCL, whole cell lysate.
Pgex 4t 1 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst+pull-downs+pgex4t-1/pcDNA3%2E1(%2B)+Laccase2+MCS+Exon+Vector+(Plasmid+%2369893)/pmc09224168-112-24-26
Average 96 stars, based on 1 article reviews
pgex 4t 1 vector - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
pgex4t 3  (Danaher Inc)
96
Danaher Inc pgex4t 3
CDC73 binds directly to USP37. Schematic diagram of USPs and CDC73 expression vectors ( A ). For GST pull-down analysis, full-length cDNAs encoding USP37, as well as USP12, USP15, USP25 and USP28, were cloned into <t>pGEX-4T-1</t> vector. Full-length wild-type CDC73 was cloned into pGEX-4T-1, and a vector expressing only CDC73 without GST was also constructed. GST/pGEX-4T-1 was used as control. GST pull-down analysis using GST-USP37 and GST-CDC73 ( B , C ). E.coli BL21 was transformed with GST-USP37/pGEX-4T-1 or GST-CDC73/pGEX-4T-1 and induced with 100 uM IPTG for 3 h. Purified GST-USP37 was incubated with a lysate of FLAG-CDC73-transfected cells ( B ), and purified GST-CDC73 was incubated with a lysate of Myc-USP37-transfected cells ( C ). These results show that USP37 is observed to interact strongly with the CDC73 protein. GST, glutathione S-transferase; WCL, whole cell lysate.
Pgex4t 3, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst+pull-downs+pgex4t-1/PGEX-4T-3/pmc01283783-431-38-39
Average 96 stars, based on 1 article reviews
pgex4t 3 - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier

Image Search Results


CDC73 binds directly to USP37. Schematic diagram of USPs and CDC73 expression vectors ( A ). For GST pull-down analysis, full-length cDNAs encoding USP37, as well as USP12, USP15, USP25 and USP28, were cloned into pGEX-4T-1 vector. Full-length wild-type CDC73 was cloned into pGEX-4T-1, and a vector expressing only CDC73 without GST was also constructed. GST/pGEX-4T-1 was used as control. GST pull-down analysis using GST-USP37 and GST-CDC73 ( B , C ). E.coli BL21 was transformed with GST-USP37/pGEX-4T-1 or GST-CDC73/pGEX-4T-1 and induced with 100 uM IPTG for 3 h. Purified GST-USP37 was incubated with a lysate of FLAG-CDC73-transfected cells ( B ), and purified GST-CDC73 was incubated with a lysate of Myc-USP37-transfected cells ( C ). These results show that USP37 is observed to interact strongly with the CDC73 protein. GST, glutathione S-transferase; WCL, whole cell lysate.

Journal: International Journal of Molecular Sciences

Article Title: USP37 Deubiquitinates CDC73 in HPT-JT Syndrome

doi: 10.3390/ijms23126364

Figure Lengend Snippet: CDC73 binds directly to USP37. Schematic diagram of USPs and CDC73 expression vectors ( A ). For GST pull-down analysis, full-length cDNAs encoding USP37, as well as USP12, USP15, USP25 and USP28, were cloned into pGEX-4T-1 vector. Full-length wild-type CDC73 was cloned into pGEX-4T-1, and a vector expressing only CDC73 without GST was also constructed. GST/pGEX-4T-1 was used as control. GST pull-down analysis using GST-USP37 and GST-CDC73 ( B , C ). E.coli BL21 was transformed with GST-USP37/pGEX-4T-1 or GST-CDC73/pGEX-4T-1 and induced with 100 uM IPTG for 3 h. Purified GST-USP37 was incubated with a lysate of FLAG-CDC73-transfected cells ( B ), and purified GST-CDC73 was incubated with a lysate of Myc-USP37-transfected cells ( C ). These results show that USP37 is observed to interact strongly with the CDC73 protein. GST, glutathione S-transferase; WCL, whole cell lysate.

Article Snippet: For GST pull-down analysis, full-length cDNAs encoding GST fusions of USP12, USP15, USP25, USP28 and USP37 selected by yeast two-hybrid screening were cloned into pGEX-4T-1 vector (Addgene, Watertown, MA, USA) ( A).

Techniques: Expressing, Clone Assay, Plasmid Preparation, Construct, Transformation Assay, Purification, Incubation, Transfection