Journal: Pharmaceuticals

Article Title: Purpurin Rescues Contrast-Induced Acute Rat Kidney Injury via Inducing Autophagy and Inhibiting Apoptosis

doi: 10.3390/ph19010116

Figure Lengend Snippet: Biochemical Parameters Demonstrating the Amelioration of Purpurin on CIAKI. ( A ) Schematic diagram of the CIAKI SD rats model; ( B ) KW/BW; ( C , D ) Serum level of Scr and BUN; ( E ) Paller scoring of renal HE staining; ( F ) HE staining, the red arrow represents RTEC swelling, vacuolization, necrosis, and detachment, while the yellow arrow represents inflammatory cell infiltration within the renal tubules; ( G – L ) Serum level of TNF-α, IL-1β, IL-6; ( J – M ) The level of NO, MDA, SOD, GSH-PX in renal. Data were expressed as the mean ± SD, n = 6, ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The supernatants were used to analyze the malondialdehyde (MDA) (A003-1-2, Nanjing Jiancheng Bioengineering Institute, Nanjing, China) content, glutathione peroxidase (GSH-Px) (A005-1-2, Nanjing Jiancheng Bioengineering Institute, Nanjing, China) activity, superoxide dismutase (SOD) (A001-1-2, Nanjing Jiancheng Bioengineering Institute, Nanjing, China) activity, and nitric oxide (NO) (A013-2-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China) content according to manufacturers’ protocols from corresponding kits.

Techniques: Staining

Journal: Reproductive biology and endocrinology : RB&E

Article Title: Betaine attenuate chronic restraint stress-induced changes in testicular damage and oxidative stress in male mice.

doi: 10.1186/s12958-022-00949-8

Figure Lengend Snippet: Fig. 5 Biochemical analysis of antioxidant status. Each bar represents the mean ± SEM (N = 6 male mice). (A) Effect of BET on the levels of oxidized glutathione (GSSG) in the testis of CRS mice; (B) BET on the levels of CRS mice The effect of reduced glutathione (GSH) level in testis tissue; (C) The effect of BET on the content of malondialdehyde (MDA) in testis tissue of CRS mice; *P < 0.05, compared with Control group; ***P < 0.001, compared with Control group; ###P < 0.001, compared with CRS + NS group; Different symbol indicate significant difference P < 0.05 among groups determined by one-way ANOVA (followed by Bonferroni post hoc test)

Article Snippet: The homogenates were filtered and centrifuged at 3500 rpm and 4 °C for 15 min using a refrigerated centrifuge and the activities of MDA (Solarbio, BC0025, China), GSSG (Elabscience, E-BC-K097-M, China) and GSH (Elabscience, E-BC-K030-M, China) were determined according to the instruction.

Techniques: Control