Journal: Heliyon

Article Title: Association of elevated autoantibody to high expression of GNAS in hepatocellular carcinoma

doi: 10.1016/j.heliyon.2023.e22627

Figure Lengend Snippet: Workflow chart of the study. First, 125 early-stage HCC patients and 125 healthy controls were recruited to evaluate the potential value of anti-GNAS autoantibody for early detection of HCC by ELISA. GNAS protein expressions in 61 paired HCC or adjacent normal tissues were tested by IHC, subsequently, the levels of GNAS protein in three HCC cell lines and the normal liver cell line were investigated by Western blot. GEO datasets were inquired to explore the mRNA expression level of GNAS, meanwhile, the mRNA levels in three HCC cell lines and the normal liver cell line were detected by real-time PCR. Additionally, ICGC Data Portal was also queried for exploring the mutation frequency of GNAS in HCC tissues.

Article Snippet: IHC assay was performed by using mouse monoclonal antibody against GNAS (Proteintech, China, 1:100 dilution) according to the manufacturer's recommendations to analyze GNAS protein expression level.

Techniques: Biomarker Discovery, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Real-time Polymerase Chain Reaction, Mutagenesis

Journal: Heliyon

Article Title: Association of elevated autoantibody to high expression of GNAS in hepatocellular carcinoma

doi: 10.1016/j.heliyon.2023.e22627

Figure Lengend Snippet: The scatter plot of autoantibody to GNAS in different groups (A), and ROC curves of autoantibody to GNAS when distinguishing early HCC and different subgroups of HCC from healthy control (B, C, D, E, F). HCC, hepatocellular carcinoma; AFP, alpha-feto-protein; ELISA, Enzyme-linked immunosorbent assay; HCC(I), HCC patients of TNM stage I; HCC(II), HCC patients of TNM stage II; HCC_AFP (+), HCC patients of AFP value greater than or equal to 20 ng/mL; HCC_AFP (−), HCC patients of AFP value less than 20 ng/mL; Se, sensitivity; Sp, specificity; AUC, area under the receiver operating characteristic curve; 95 % confidence interval of AUC in brackets.

Article Snippet: IHC assay was performed by using mouse monoclonal antibody against GNAS (Proteintech, China, 1:100 dilution) according to the manufacturer's recommendations to analyze GNAS protein expression level.

Techniques: Control, Enzyme-linked Immunosorbent Assay

Journal: Heliyon

Article Title: Association of elevated autoantibody to high expression of GNAS in hepatocellular carcinoma

doi: 10.1016/j.heliyon.2023.e22627

Figure Lengend Snippet: The levels and frequency of autoantibody to GNAS evaluated by ELISA.

Article Snippet: IHC assay was performed by using mouse monoclonal antibody against GNAS (Proteintech, China, 1:100 dilution) according to the manufacturer's recommendations to analyze GNAS protein expression level.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Heliyon

Article Title: Association of elevated autoantibody to high expression of GNAS in hepatocellular carcinoma

doi: 10.1016/j.heliyon.2023.e22627

Figure Lengend Snippet: the expression of GNAS evaluated by IHC.

Article Snippet: IHC assay was performed by using mouse monoclonal antibody against GNAS (Proteintech, China, 1:100 dilution) according to the manufacturer's recommendations to analyze GNAS protein expression level.

Techniques: Expressing

Journal: Heliyon

Article Title: Association of elevated autoantibody to high expression of GNAS in hepatocellular carcinoma

doi: 10.1016/j.heliyon.2023.e22627

Figure Lengend Snippet: the expression of GNAS in tissue and cell lines. (A) Immunohistochemical staining of GNAS in HCC tissue and adjacent normal tissue slides. Positive stain pattern of GNAS in representative HCC tissue of TNM stage I, weak stain pattern of GNAS in the representative adjacent normal tissue of TNM stage I (40× and 200 × magnifications) (B) the cropped gel displaying protein levels of GNAS tested by western blotting in cell lines. The lanes of upper lines from left to right showed the GNAS protein levels in cell lines of L02, SNU449, HepG2, and Hep3b, and the following lanes were the expression levels of reference protein GADPH. The corresponding uncropped full-length gel can be found in Additional file 1. (C) the relative expression of GNAS to GAPDH in protein level from a normal liver cell line (L02) and three HCC cell lines (SUN449, HepG2, and Hep3b). (D) the relative expression of GNAS to GAPDH in mRNA level from normal liver cell line (L02) and three HCC cell lines (SUN449, HepG2 and Hep3b), **, P < 0.01, ***, P < 0.001. (E) the expressions of GNAS at the mRNA level from three datasets of the GEO database, numbers in horizontal-axis parentheses represent the sample size.

Article Snippet: IHC assay was performed by using mouse monoclonal antibody against GNAS (Proteintech, China, 1:100 dilution) according to the manufacturer's recommendations to analyze GNAS protein expression level.

Techniques: Expressing, Immunohistochemical staining, Staining, Western Blot

Journal: Heliyon

Article Title: Association of elevated autoantibody to high expression of GNAS in hepatocellular carcinoma

doi: 10.1016/j.heliyon.2023.e22627

Figure Lengend Snippet: Frequency of GNAS Mutations in HCC across 5 cohorts.

Article Snippet: IHC assay was performed by using mouse monoclonal antibody against GNAS (Proteintech, China, 1:100 dilution) according to the manufacturer's recommendations to analyze GNAS protein expression level.

Techniques: Virus