Journal: Light, Science & Applications

Article Title: Near-infrared light activatable chemically induced CRISPR system

doi: 10.1038/s41377-025-01917-8

Figure Lengend Snippet: a Custom LED illumination setup. b Fluorescence of eGFP-expressing HEK293FT cells treated with rapamycin dimer, transfected with full-length dCas9. At the top of the setup there is a holder for a 35 mm glass-bottom dish. The 3D-printed double slit screen is located in the focal plane of the illumination setup. c Fluorescence of eGFP-expressing HEK293FT cells treated with rapamycin dimer, transfected with split dCas9 without NIR illumination. d Fluorescence of eGFP-expressing HEK293FT cells treated with rapamycin dimer, transfected with split dCas9 under 30 seconds of NIR illumination. e 3D-printed double-slit screen with each slit being 500 μm wide and 2 mm long. f NIR illumination using double slit screen. g Corresponding pattern of the fluorescence of eGFP-expressing cells

Article Snippet: The plasmids encoding Cas9 (PX851, #62883 and PX852, #62884), split dCas9 (PX855, #62887 and PX856, #62888), dCas9-VP64 (#61425), an eGFP-expressing surrogate reporter (#60718), and a sgRNA targeting the eGFP reporter (#60719) were obtained from Addgene.

Techniques: Fluorescence, Expressing, Transfection