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Image Search Results
Journal: Frontiers in Plant Science
Article Title: DspA/E Contributes to Apoplastic Accumulation of ROS in Non-host A. thaliana
doi: 10.3389/fpls.2016.00545
Figure Lengend Snippet: Erwinia amylovora induces accumulation of H 2 O 2 . (A–C) Leaves of 5-weeks-old plants were infiltrated on the upper half of the leaf blade with water (mock), E. amylovora ( Ea ) wild-type strain, T3SS-deficient mutant ( tts ) or dspA/E mutant ( dspA/E ). (A) Leaves stained with DCFH-DA show intracellular H 2 O 2 accumulation in green. (B) Brown stains indicate accumulation of H 2 O 2 in leaves stained with DAB, the graph to the right shows DAB staining intensity. The stars indicate a significant difference according to Mann and Whitney’s statistical test ( p < 0.05). (C) Localization of H 2 O 2 deposits in DAB-stained leaves observed under a photon microscope. Bar: 50 μm; pl: plastid; pe: staining at the periphery of the cell. Similar results were obtained for three independent experiments. Top panel: palissadic parenchyma; bottom panel: spongy parenchyma.
Article Snippet: Cut samples were collected on
Techniques: Mutagenesis, Staining, Microscopy