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Image Search Results
Journal: The Journal of Biological Chemistry
Article Title: CDP-DAG synthase 1 and 2 regulate lipid droplet growth through distinct mechanisms
doi: 10.1074/jbc.RA119.009992
Figure Lengend Snippet: Knocking down DGAT2 or GPAT4 restored normal LD morphology in CDS2-deficient cells. A, mRNA levels of DGAT1/2 and GPAT3/4 upon CDS KD in HeLa cells. Two-tailed Student's t test was used: mean ± S.D. (error bars); n = 3; **, p < 0.01; ****, p < 0.0001. B, KD efficiency of DGAT1/2 and GPAT3/4 in HeLa cells. Two-tailed Student's t test was used: mean ± S.D.; n = 3; **, p < 0.01; ***, p < 0.001. Two different sequences (a and b) of siRNA against each gene were used. C, LD morphology when DGAT1/2 were knocked down in CDS-deficient HeLa cells. 200 μm oleate was added to cells to induce LD formation for 16 h. LDs were stained by BODIPY. Bars, 5 μm. D, distribution of LDs according to their sizes in C. LDs from ∼50 cells/cell type were used. E, LD morphology when DGAT3/4 were knocked down in CDS-deficient HeLa cells. 200 μm oleate was added to cells to induce LD formation for 16 h. LDs were stained by BODIPY. Bars, 5 μm. F, distribution of LDs according to their sizes in E. LDs from ∼50 cells/cell type were used. G, quantification of the diameters of the three largest LDs in each cell as shown in C and E. Two-tailed Student's t test was used: mean ± S.D.; n = 45 LDs from 15 cells for each cell type; *, p < 0.05; **, p < 0.01; ****, p < 0.0001; ns, no significance. H, total TAG level in CDS1/2 and DGAT1/2 KD cells. Two-tailed Student's t test was used: mean ± S.D.; n = 3; **, p < 0.01. I, total TAG level in CDS1/2 and GPAT3/4 KD cells. Two-tailed Student's t test was used: mean ± S.D.; n = 3; **, p < 0.01.
Article Snippet: Transient transfections of siRNA against CDS1/2 (Sigma-Aldrich), CIDEA/B/C (Shanghai GenePharma Co. Ltd.),
Techniques: Two Tailed Test, Staining
Journal: The Journal of Biological Chemistry
Article Title: CDP-DAG synthase 1 and 2 regulate lipid droplet growth through distinct mechanisms
doi: 10.1074/jbc.RA119.009992
Figure Lengend Snippet: CDS2 deficiency promoted LD-association of GPAT4. A, localization of GPAT3 in CDS KD cells after incubation with 200 μm oleate for 16 h. Bars, 5 μm. B, quantification of LD sizes in cells transfected by GFP-GPAT3 upon CDS deficiency. Two-tailed Student's t test was used: mean, n = ∼200–300 LDs from ∼20 cells. *, p < 0.05; **, p < 0.01; ***, p < 0.001. C, distribution of LDs in cells transfected by GFP-GPAT3 upon CDS deficiency. LDs from ∼20 cells/cell type were used. D, localization of GPAT4 in CDS KD cells at the early stage of LD formation in CDS KD cells. 200 μm oleate was added to cells for the indicated time. Bars, 5 μm. Inset, bars, 1 μm. E, quantification of size (a and c) and distribution (b and d) of GPAT4-positive LDs at 3 and 8 h after adding oleate. Two-tailed Student's t test was used: mean; n = ∼200–350 LDs from ∼20 cells; ***, p < 0.001; ns, no significance. F, quantification of GPAT4-LD associations during LD growth upon CDS deficiency: mean ± S.E. (error bars); n = 20 cells. OA, oleate.
Article Snippet: Transient transfections of siRNA against CDS1/2 (Sigma-Aldrich), CIDEA/B/C (Shanghai GenePharma Co. Ltd.),
Techniques: Incubation, Transfection, Two Tailed Test