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Biopeptide
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ProteoGenix
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Image Search Results
Journal: iScience
Article Title: Basal level of ATF4 promotes T cell readiness for activation-induced proliferation
doi: 10.1016/j.isci.2025.114277
Figure Lengend Snippet: Atf4 −/− CD8 + T cells have impaired antiviral responses (A) Transcriptome comparison of Atf4 expression in P14 T cells at 7 days post-LCMV infection. The bulk RNA sequencing data of P14 transgenic T cells at 7 days after LCMV Armstrong or CL13 infection, with the accession number GSE11943 , were analyzed. (B–E) WT and cKO mice were infected with LCMV CL13 at 4 × 10 5 PFU via intraperitoneal injection and were analyzed at 10 dpi. (B) Quantitative RT-PCR of LCMV-glycoprotein (GP) mRNA in peripheral blood and indicated tissues at 10 days post-infection (dpi). The mRNA series of dilutions of stock LCMV with known viral titers was used as a standard curve for calculating the viral titer in peripheral blood samples. N = 2–3. (C) Flow cytometry analysis of the percentages and cell numbers of total CD8 + T cells and CX3CR1 + SLAMF6 - CD8 + T cells in the spleen, liver, and lung of WT and cKO mice. N = 4. (D) Flow cytometry analysis of T-bet and Ki67 expression in CD8 + T cells. N = 4. (E) Flow cytometry analysis of IFN-γ and TNF-α expression in CD8 + T cells. N = 5. Data are representative of 3–4 independent experiments for (B-E). Student’s t test was used for statistical analysis. Mean ± SD, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.005, ∗∗∗∗ p < 0.001, ns, not significant.
Article Snippet:
Techniques: Comparison, Expressing, Infection, RNA Sequencing, Transgenic Assay, Injection, Quantitative RT-PCR, Flow Cytometry
Journal: Cell reports
Article Title: Epithelial organoid supports resident memory CD8 T cell differentiation
doi: 10.1016/j.celrep.2024.114621
Figure Lengend Snippet:
Article Snippet:
Techniques: Recombinant, Virus, Expressing, Cell Isolation, Comparison, Infection, Software