Journal: Scientific Reports

Article Title: AT-101 inhibits the proliferation and invasion of tongue squamous carcinoma cells by targeting the miR-21-5p/ FDX1 axis

doi: 10.1038/s41598-026-37710-9

Figure Lengend Snippet: Analysis of AT-101 target miRNAs and mRNAs. ( A ) Analysis of overlapping significantly differentially expressed miRNAs from the GSE28100 , GSE137865 , and TCGA miRNA microarray datasets. ( B – D ) Prediction of genes interacting with AT-101 and differentially expressed miRNAs using TargetScan and miRanda software.

Article Snippet: AT-101 (Cat No.HY-15464 A) was purchased from MCE (Shanghai, China).

Techniques: Microarray, Software

Journal: Scientific Reports

Article Title: AT-101 inhibits the proliferation and invasion of tongue squamous carcinoma cells by targeting the miR-21-5p/ FDX1 axis

doi: 10.1038/s41598-026-37710-9

Figure Lengend Snippet: AT-101 inhibited TSCC cell proliferation via miR-21-5p. ( A ) The IC50 of AT-101 in Cal27 cells was determined using a CCK-8 assay after 48 h of treatment with AT-101(0, 10, 20, 40 µM).The calculated IC50 was 12 µM. ( B ) Viability of HaCaT and Cal27 cells after treatment with 15 µM AT-101 for 48 h. ( C , D ) RT–qPCR analysis of miR-21-5p expression in Cal27 cells after AT-101 treatment and transfection with miR-21-5p mimics/inhibitors. ( E , F ) CCK-8 assay results showing that AT-101 treatment significantly inhibited Cal27 cell proliferation, whereas miR-21-5p overexpression reversed this inhibitory effect. ( G , I ) Fluorescence expression levels between different groups in the EdU labelling experiment. (Hoechst: nuclear staining; EdU: red fluorescence indicating proliferating cells). ( H , J ) Quantitative analysis of the cell proliferation rate. Data are presented as the mean ± SD, with n = 3 biological replicates per group. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: AT-101 (Cat No.HY-15464 A) was purchased from MCE (Shanghai, China).

Techniques: CCK-8 Assay, Quantitative RT-PCR, Expressing, Transfection, Over Expression, Fluorescence, Staining

Journal: Scientific Reports

Article Title: AT-101 inhibits the proliferation and invasion of tongue squamous carcinoma cells by targeting the miR-21-5p/ FDX1 axis

doi: 10.1038/s41598-026-37710-9

Figure Lengend Snippet: AT-101 affects the migration and invasion abilities of Cal27 cells by regulating miR-21-5p. ( A , C ) Wound healing assays showing the migratory ability of Cal27 cells at 0 h and 48 h under different treatment conditions. ( B , D ) Quantitative analysis of the wound closure rate at 48 h. ( E , G ) Representative images of Transwell invasion assays in each experimental group. ( F , H ) Statistical analysis of the number of invasive cells. Data are presented as the mean ± SD, with n = 3 biological replicates per group. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: AT-101 (Cat No.HY-15464 A) was purchased from MCE (Shanghai, China).

Techniques: Migration

Journal: Scientific Reports

Article Title: AT-101 inhibits the proliferation and invasion of tongue squamous carcinoma cells by targeting the miR-21-5p/ FDX1 axis

doi: 10.1038/s41598-026-37710-9

Figure Lengend Snippet: FDX1 inhibits the growth of tongue squamous cell carcinoma. ( A ) RT‒qPCR analysis of relative FDX1 mRNA expression levels in different groups. ( B ) Body weight% of BALB/c nude mice in the CTRL, sh FDX1 and FDX1 groups. ( C ) Morphological diagram of the tumour volume. ( D ) Statistical curve of the tumour growth volume. ( E ) The weight of tumours in each group. ( F ) HE staining of paraffin-embedded Sect. (4 μm) of tumour tissue (scale: 50 μm), immunofluorescence detection of Ki-67, and TUNEL staining (scale: 30 μm). ( G , H ) Quantification of immunofluorescence results. Ki-67 and TUNEL-positive areas were quantified at 400× magnification by ImageJ software. * p < 0.05, ** p < 0.01, *** p < 0.001 versus the control group. ( I ) The cartoon mechanism diagram: AT-101 inhibits proliferation and invasion of tongue squamous carcinoma cells by targeting the miR-21-5p/FDX1 axis (upper); FDX1 inhibits the growth of tongue squamous cell carcinoma (lower).

Article Snippet: AT-101 (Cat No.HY-15464 A) was purchased from MCE (Shanghai, China).

Techniques: Expressing, Staining, Immunofluorescence, TUNEL Assay, Software, Control