Journal: Genes

Article Title: Chromosome 4 Duplication Associated with Strabismus Leads to Gene Expression Changes in iPSC-Derived Cortical Neurons

doi: 10.3390/genes16010080

Figure Lengend Snippet: Heatmap of the DEGs in neuroprogenitors ( A ) and cortical neurons ( B ) and validation by qPCR ( C ). ( A ) Heatmap showing the expressions (z-scores) of differentially expressed genes in neuroprogenitor cells between controls and cells with duplication (fourth independent differentiations). ( B ) Heatmap showing the expressions (z-scores) of differentially expressed genes in cortical neurons between controls and cells with duplication, a pink brain next to the gene name represents a neuronal/synapse related function and a blue brain next to the gene name represents genes associated with neurodevelopmental disorders. ( C ) qRT-PCR validation from randomly selected upregulated and downregulated genes; total RNA from genotypes at day 14 and day 63 was used to analyze expression levels, and data were normalized using TBP as the reference gene.

Article Snippet: Then, 200 ng of high-quality RNA from each sample was used for total RNA sequencing library preparation with the TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Plus for Human/Mouse/Rat (Cat. No. RS-122-2201 (Illumina Inc., San Diego, CA, USA), following the manufacturer’s instructions.

Techniques: Biomarker Discovery, Quantitative RT-PCR, Expressing