Journal: Vaccines

Article Title: Simultaneous Administration of Recombinant Measles Viruses Expressing Respiratory Syncytial Virus Fusion (F) and Nucleo (N) Proteins Induced Humoral and Cellular Immune Responses in Cotton Rats

doi: 10.3390/vaccines7010027

Figure Lengend Snippet: The proportion of CD8 + /IFN-γ + cells in spleen cells obtained four days after the RSV challenge (Day 88), stimulated with UV-inactivated RSV Long strain, F, NP, and M2-1 peptides. Spleen cells were obtained after the RSV challenge and analyzed for IFN-γ production by CD8 + cells. The bar represents the mean of 3~4 rats per group ± SEM. (* p < 0.05).

Article Snippet: After the stimulation, splenocytes were washed and incubated with an anti-CD8 antibody (R&D Systems, Minneapolis, MN, USA) at 4 °C for 30 min. Splenocytes were fixed with fixation buffer in the Cytofix/cytoperm kit, and intracellular cytokines were stained with a goat IgG antibody against IFN-γ (R&D Systems, USA) and anti-goat IgG PE-Cy7 (Santa Cruz Biotechnology, Inc., Dallas, TX, USA) at 4 °C for 60 min. Cellular populations were analyzed using flow cytometry with Cytomics FC 500 (Beckman Coulter, Inc., Indianapolis, IN, USA) and counted until 100,000 cells.

Techniques:

Journal: Vaccines

Article Title: Simultaneous Administration of Recombinant Measles Viruses Expressing Respiratory Syncytial Virus Fusion (F) and Nucleo (N) Proteins Induced Humoral and Cellular Immune Responses in Cotton Rats

doi: 10.3390/vaccines7010027

Figure Lengend Snippet: Detection of IFN-γ + expressing cells in lung homogenates. Lung homogenates were obtained after the RSV challenge on Day 88. IFN-γ-producing cells were separated further into CD8 + and CD4 + cells. The bar represents the mean of 3~4 rats per group ± SEM. (* p < 0.05).

Article Snippet: After the stimulation, splenocytes were washed and incubated with an anti-CD8 antibody (R&D Systems, Minneapolis, MN, USA) at 4 °C for 30 min. Splenocytes were fixed with fixation buffer in the Cytofix/cytoperm kit, and intracellular cytokines were stained with a goat IgG antibody against IFN-γ (R&D Systems, USA) and anti-goat IgG PE-Cy7 (Santa Cruz Biotechnology, Inc., Dallas, TX, USA) at 4 °C for 60 min. Cellular populations were analyzed using flow cytometry with Cytomics FC 500 (Beckman Coulter, Inc., Indianapolis, IN, USA) and counted until 100,000 cells.

Techniques: Expressing