Journal: International Journal of Molecular Sciences

Article Title: Rheumatoid Arthritis-Associated MicroRNA-155 Targets SOCS1 and Upregulates TNF-α and IL-1β in PBMCs

doi: 10.3390/ijms141223910

Figure Lengend Snippet: Demographic, clinical, and laboratory data of patients with rheumatoid arthritis, osteoarthritis, and controls.

Article Snippet: The levels of TNF-α and IL-1β in plasma and PBMCs supernatant were measured by an enzyme immunoassay using the human TNF-α and IL-1β ELISA Kit (Sino Biological Inc., Beijing, China).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Rheumatoid Arthritis-Associated MicroRNA-155 Targets SOCS1 and Upregulates TNF-α and IL-1β in PBMCs

doi: 10.3390/ijms141223910

Figure Lengend Snippet: miR-155 upregulation correlates with increased production of TNF-α and IL-1β in Rheumatoid arthritis (RA) patients. ( A ) and ( B ): Increased expression of TNF-α and IL-1β in RA plasma. Results are expressed as pg/mL by an ELISA test; ( C ): MiR-155 is upregulated in PBMCs of active RA patients. Results in patients with RA ( n = 45) and OA ( n = 32) are shown as fold increase relative to the control group ( n = 25); ( D ) and ( E ): Correlations between Relative miR-155 expression with the TNF-α or IL-1β expression. RA, rheumatoid arthritis; OA, osteoarthritis. Statistical significance was considered with a p value < 0.05.

Article Snippet: The levels of TNF-α and IL-1β in plasma and PBMCs supernatant were measured by an enzyme immunoassay using the human TNF-α and IL-1β ELISA Kit (Sino Biological Inc., Beijing, China).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: International Journal of Molecular Sciences

Article Title: Rheumatoid Arthritis-Associated MicroRNA-155 Targets SOCS1 and Upregulates TNF-α and IL-1β in PBMCs

doi: 10.3390/ijms141223910

Figure Lengend Snippet: Correlations between miR-155 expression and the various clinical and laboratory data of patients with rheumatoid arthritis ( n = 45).

Article Snippet: The levels of TNF-α and IL-1β in plasma and PBMCs supernatant were measured by an enzyme immunoassay using the human TNF-α and IL-1β ELISA Kit (Sino Biological Inc., Beijing, China).

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Rheumatoid Arthritis-Associated MicroRNA-155 Targets SOCS1 and Upregulates TNF-α and IL-1β in PBMCs

doi: 10.3390/ijms141223910

Figure Lengend Snippet: miR-155 promotes production of TNF-α and IL-1β in human PBMCs. ( A ) Fold changes of miR-155 expression were determined by qRT-PCR after the transfection of miR-control, or miR-155 mimics at 25 or 50 nM for 24 h; ( B ) and ( C ) Expression of TNF-α and IL-1β in supernatant of PBMCs 24 h post LPS treatment, or 24 h post transfection with 25 nM miR-155 mimics, 50 nM miR-155 mimics or 50 nM miRNA control. Results were expressed as pg/mL by an ELISA test; and ( D ) mRNA expression of TNF-α (group 1) and IL-1β (group 2) in PBMCs 24 h after LPS treatment or 24 h post transfection with 25 nM miR-155 mimics, 50 nM miR-155 mimics or 50 nM miRNA control. All results are the average of three independent experiments. Statistical significance is shown.

Article Snippet: The levels of TNF-α and IL-1β in plasma and PBMCs supernatant were measured by an enzyme immunoassay using the human TNF-α and IL-1β ELISA Kit (Sino Biological Inc., Beijing, China).

Techniques: Expressing, Quantitative RT-PCR, Transfection, Enzyme-linked Immunosorbent Assay

Journal: International Journal of Molecular Sciences

Article Title: Rheumatoid Arthritis-Associated MicroRNA-155 Targets SOCS1 and Upregulates TNF-α and IL-1β in PBMCs

doi: 10.3390/ijms141223910

Figure Lengend Snippet: miR-155 inhibitor reduces the production of proinflammatory cytokines induced by LPS. ( A ) Fold changes in miR-155 expression were determined by RT-qPCR 24 h post transfection with 50 nM miRNA inhibitor control, 25 or 50 nM miR-155 inhibitor; ( B ) and ( C ) Expression of TNF-α and IL-1β in supernatant of PBMCs 24 h after LPS treatment, or (and) 24 h post transfection with 5 nM miR-155 inhibitor or 10 nM miR-155 inhibitor. Results were expressed as pg/mL by an ELISA test; and ( D ) mRNA expression of TNF-α (group 1) and IL-1β (group 2) in PBMCs 24 h after LPS treatment, or (and) 24 h post transfection with 10 nM miRNA inhibitor control, 5 nM miR-155 inhibitor or 10 nM miRNA inhibitor. All results are the average of three independent experiments. Statistical significance is shown.

Article Snippet: The levels of TNF-α and IL-1β in plasma and PBMCs supernatant were measured by an enzyme immunoassay using the human TNF-α and IL-1β ELISA Kit (Sino Biological Inc., Beijing, China).

Techniques: Expressing, Quantitative RT-PCR, Transfection, Enzyme-linked Immunosorbent Assay

Journal: Cell reports

Article Title: Mutant CHCHD10 causes an extensive metabolic rewiring that precedes OXPHOS dysfunction in a murine model of mitochondrial cardiomyopathy

doi: 10.1016/j.celrep.2022.110475

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit anti-GMPS (1:500) , ProteinTech , Cat# 16376–1-AP; RRID: AB_2232412.

Techniques: Transduction, Recombinant, Western Blot, Multiple Displacement Amplification, Reverse Transcription, Bradford Protein Assay, DNA Purification, Gene Expression, Software

Journal: PeerJ

Article Title: Quality assessment of a serum and xenofree medium for the expansion of human GMP-grade mesenchymal stromal cells

doi: 10.7717/peerj.13391

Figure Lengend Snippet: Supernatant of THP-1 cells co-cultured with MSC at P1 and P2 was assayed for TNF-α and IL-1ra secretion. The cytokine concentration is normalized by the one measured in the LPS-treated THP-1 monoculture . Data are presented as median ± interquartile range with n = 7 for TNF- α secretion by BM-MSC, n = 6 for IL-1ra secretion by BM-MSC, n = 5 for TNF- α secretion by Ad-MSC and n = 4 for IL-1ra secretion by Ad-MSC. Friedman test was used to compare the effect of MSC expanded in the 2 media (hCPL and SXFM) to THP-1 stimulated by LPS, ns = non-significant, ∗ p < 0.05 and ∗∗ p < 0.01.

Article Snippet: As highly encouraged by the regulators to have a backup media in all Good Manufacturing Practices (GMP) processes (Guideline on human cell-based medicinal products, EMEA/CHMP/410869/2006), the aim of this study was to validate the use of a GMP standardized SXFM medium: the MSC-Brew GMP Medium (Miltenyi Biotec ® , Bergisch Gladbach, Germany).

Techniques: Cell Culture, Concentration Assay

Journal: Mediators of Inflammation

Article Title: Associations of Trauma Severity with Mean Platelet Volume and Levels of Systemic Inflammatory Markers (IL1 β , IL6, TNF α , and CRP)

doi: 10.1155/2016/9894716

Figure Lengend Snippet: Comparison of biochemical markers within between study groups and controls.

Article Snippet: Serum TNF α , IL1 β , and IL6 levels were measured using Boster ELISA kits (Freemont, CA, USA) and Bio-Tek (Winooski, VT, USA) ELx50 and ELx800 devices.

Techniques: Comparison, Control