glycerol Search Results


pdcd6ip gene  (Revvity)
91
Revvity pdcd6ip gene
Pdcd6ip Gene, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glycerol/Glycerol/pmc09974061-341-15-20
Average 91 stars, based on 1 article reviews
pdcd6ip gene - by Bioz Stars, 2026-09
91/100 stars
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doxdhcer m18  (Croda International Plc)
94
Croda International Plc doxdhcer m18

Doxdhcer M18, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glycerol/17%3A0-17%3A1-17%3A0+D5+TG/pmc10772323-359-24-41
Average 94 stars, based on 1 article reviews
doxdhcer m18 - by Bioz Stars, 2026-09
94/100 stars
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phosphatidylglycerol pg  (Croda International Plc)
96
Croda International Plc phosphatidylglycerol pg

Phosphatidylglycerol Pg, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glycerol/18%3A1+(%CE%949-Cis)+PG/pmc06855803-42-5-8
Average 96 stars, based on 1 article reviews
phosphatidylglycerol pg - by Bioz Stars, 2026-09
96/100 stars
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c11  (Croda International Plc)
90
Croda International Plc c11
dga t2 mutant embryos are still capable of triacylglycerol synthesis in the YSL. A , TopFluor <t>C11</t> fatty acid was mixed with canola oil and microinjected into the yolk mass of dgat2 sa13945 mutants and siblings at 3 dpf. B , representative image of the TopFluor C11 signal in a dgat2 sa13945 zebrafish yolk sac 4 h after injection; scale = 200 μm. C and D , lipids were extracted from TopFluor C11 injected embryos and separated by thin layer chromatography (20 pooled embryos per sample, wild-type and dgat2 sa13945/+ embryos were combined). C , lipid classes were identified using TopFluor-labeled lipid standards. Arrow indicates TAG synthesized from TopFlour C11 in vivo . (CE: cholesterol ester, TAG: triacylglycerol, DAG: diacylglycerol, MAG: monoacylglycerol; PL: phospholipid; ∗ denote autofluorescent lipids in the fish lysate). D , the quantity of lipids in each class was quantified and expressed as a percent of total fluorescent lipids; N = 6 experiments, mean ± SD, Two-way ANOVA, not significantly different, p = 0.9929. Note, it appears that the TopFluor C11 is unable to be incorporated into cholesterol esters. E , large lipid droplets were manually dissected out of dgat2 sa13945 larvae at 6 dpf (40–50 LDs from 30 – 40 larvae). Lipids in LDs or an equivalent volume of media surrounding dissected larvae were separated by multistep gradient HPLC and detected with a charged aerosol detector (CAD) in picoamperes (pA) as described in . The lipid droplet samples contain large quantities of triacylglycerols and cholesterol esters. Traces are representative of three independent experiments.
C11, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glycerol/18%3A1-18%3A1-C11+TopFluor+TG/pmc11663968-508-9-28
Average 90 stars, based on 1 article reviews
c11 - by Bioz Stars, 2026-09
90/100 stars
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myristoyl 2 hydroxy sn glycero 3 phospho  (Croda International Plc)
94
Croda International Plc myristoyl 2 hydroxy sn glycero 3 phospho
dga t2 mutant embryos are still capable of triacylglycerol synthesis in the YSL. A , TopFluor <t>C11</t> fatty acid was mixed with canola oil and microinjected into the yolk mass of dgat2 sa13945 mutants and siblings at 3 dpf. B , representative image of the TopFluor C11 signal in a dgat2 sa13945 zebrafish yolk sac 4 h after injection; scale = 200 μm. C and D , lipids were extracted from TopFluor C11 injected embryos and separated by thin layer chromatography (20 pooled embryos per sample, wild-type and dgat2 sa13945/+ embryos were combined). C , lipid classes were identified using TopFluor-labeled lipid standards. Arrow indicates TAG synthesized from TopFlour C11 in vivo . (CE: cholesterol ester, TAG: triacylglycerol, DAG: diacylglycerol, MAG: monoacylglycerol; PL: phospholipid; ∗ denote autofluorescent lipids in the fish lysate). D , the quantity of lipids in each class was quantified and expressed as a percent of total fluorescent lipids; N = 6 experiments, mean ± SD, Two-way ANOVA, not significantly different, p = 0.9929. Note, it appears that the TopFluor C11 is unable to be incorporated into cholesterol esters. E , large lipid droplets were manually dissected out of dgat2 sa13945 larvae at 6 dpf (40–50 LDs from 30 – 40 larvae). Lipids in LDs or an equivalent volume of media surrounding dissected larvae were separated by multistep gradient HPLC and detected with a charged aerosol detector (CAD) in picoamperes (pA) as described in . The lipid droplet samples contain large quantities of triacylglycerols and cholesterol esters. Traces are representative of three independent experiments.
Myristoyl 2 Hydroxy Sn Glycero 3 Phospho, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glycerol/14%3A0+Lyso+PG/pmc04239197-55-8-20
Average 94 stars, based on 1 article reviews
myristoyl 2 hydroxy sn glycero 3 phospho - by Bioz Stars, 2026-09
94/100 stars
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materials 1 palmitoyl 2 oleoyl sn glycero 3 phospho  (Croda International Plc)
96
Croda International Plc materials 1 palmitoyl 2 oleoyl sn glycero 3 phospho
dga t2 mutant embryos are still capable of triacylglycerol synthesis in the YSL. A , TopFluor <t>C11</t> fatty acid was mixed with canola oil and microinjected into the yolk mass of dgat2 sa13945 mutants and siblings at 3 dpf. B , representative image of the TopFluor C11 signal in a dgat2 sa13945 zebrafish yolk sac 4 h after injection; scale = 200 μm. C and D , lipids were extracted from TopFluor C11 injected embryos and separated by thin layer chromatography (20 pooled embryos per sample, wild-type and dgat2 sa13945/+ embryos were combined). C , lipid classes were identified using TopFluor-labeled lipid standards. Arrow indicates TAG synthesized from TopFlour C11 in vivo . (CE: cholesterol ester, TAG: triacylglycerol, DAG: diacylglycerol, MAG: monoacylglycerol; PL: phospholipid; ∗ denote autofluorescent lipids in the fish lysate). D , the quantity of lipids in each class was quantified and expressed as a percent of total fluorescent lipids; N = 6 experiments, mean ± SD, Two-way ANOVA, not significantly different, p = 0.9929. Note, it appears that the TopFluor C11 is unable to be incorporated into cholesterol esters. E , large lipid droplets were manually dissected out of dgat2 sa13945 larvae at 6 dpf (40–50 LDs from 30 – 40 larvae). Lipids in LDs or an equivalent volume of media surrounding dissected larvae were separated by multistep gradient HPLC and detected with a charged aerosol detector (CAD) in picoamperes (pA) as described in . The lipid droplet samples contain large quantities of triacylglycerols and cholesterol esters. Traces are representative of three independent experiments.
Materials 1 Palmitoyl 2 Oleoyl Sn Glycero 3 Phospho, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glycerol/16%3A0-18%3A1+PG/10__5599_slash_admet__5__3__400-34-0-16
Average 96 stars, based on 1 article reviews
materials 1 palmitoyl 2 oleoyl sn glycero 3 phospho - by Bioz Stars, 2026-09
96/100 stars
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tag  (Croda International Plc)
94
Croda International Plc tag
dga t2 mutant embryos are still capable of triacylglycerol synthesis in the YSL. A , TopFluor <t>C11</t> fatty acid was mixed with canola oil and microinjected into the yolk mass of dgat2 sa13945 mutants and siblings at 3 dpf. B , representative image of the TopFluor C11 signal in a dgat2 sa13945 zebrafish yolk sac 4 h after injection; scale = 200 μm. C and D , lipids were extracted from TopFluor C11 injected embryos and separated by thin layer chromatography (20 pooled embryos per sample, wild-type and dgat2 sa13945/+ embryos were combined). C , lipid classes were identified using TopFluor-labeled lipid standards. Arrow indicates TAG synthesized from TopFlour C11 in vivo . (CE: cholesterol ester, TAG: triacylglycerol, DAG: diacylglycerol, MAG: monoacylglycerol; PL: phospholipid; ∗ denote autofluorescent lipids in the fish lysate). D , the quantity of lipids in each class was quantified and expressed as a percent of total fluorescent lipids; N = 6 experiments, mean ± SD, Two-way ANOVA, not significantly different, p = 0.9929. Note, it appears that the TopFluor C11 is unable to be incorporated into cholesterol esters. E , large lipid droplets were manually dissected out of dgat2 sa13945 larvae at 6 dpf (40–50 LDs from 30 – 40 larvae). Lipids in LDs or an equivalent volume of media surrounding dissected larvae were separated by multistep gradient HPLC and detected with a charged aerosol detector (CAD) in picoamperes (pA) as described in . The lipid droplet samples contain large quantities of triacylglycerols and cholesterol esters. Traces are representative of three independent experiments.
Tag, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glycerol/15%3A0-18%3A1-d7-15%3A0+TG/10__17816_slash_cp15491-93-20-22
Average 94 stars, based on 1 article reviews
tag - by Bioz Stars, 2026-09
94/100 stars
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dioleoyl sn glycero 3 phospho  (Croda International Plc)
92
Croda International Plc dioleoyl sn glycero 3 phospho
dga t2 mutant embryos are still capable of triacylglycerol synthesis in the YSL. A , TopFluor <t>C11</t> fatty acid was mixed with canola oil and microinjected into the yolk mass of dgat2 sa13945 mutants and siblings at 3 dpf. B , representative image of the TopFluor C11 signal in a dgat2 sa13945 zebrafish yolk sac 4 h after injection; scale = 200 μm. C and D , lipids were extracted from TopFluor C11 injected embryos and separated by thin layer chromatography (20 pooled embryos per sample, wild-type and dgat2 sa13945/+ embryos were combined). C , lipid classes were identified using TopFluor-labeled lipid standards. Arrow indicates TAG synthesized from TopFlour C11 in vivo . (CE: cholesterol ester, TAG: triacylglycerol, DAG: diacylglycerol, MAG: monoacylglycerol; PL: phospholipid; ∗ denote autofluorescent lipids in the fish lysate). D , the quantity of lipids in each class was quantified and expressed as a percent of total fluorescent lipids; N = 6 experiments, mean ± SD, Two-way ANOVA, not significantly different, p = 0.9929. Note, it appears that the TopFluor C11 is unable to be incorporated into cholesterol esters. E , large lipid droplets were manually dissected out of dgat2 sa13945 larvae at 6 dpf (40–50 LDs from 30 – 40 larvae). Lipids in LDs or an equivalent volume of media surrounding dissected larvae were separated by multistep gradient HPLC and detected with a charged aerosol detector (CAD) in picoamperes (pA) as described in . The lipid droplet samples contain large quantities of triacylglycerols and cholesterol esters. Traces are representative of three independent experiments.
Dioleoyl Sn Glycero 3 Phospho, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glycerol/18%3A1-C11+TopFluor+-C11+TopFluor+TG/10__1074_slash_jbc__m113__530055-51-48-62
Average 92 stars, based on 1 article reviews
dioleoyl sn glycero 3 phospho - by Bioz Stars, 2026-09
92/100 stars
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distearoyl sn glycero 3 phospho  (Croda International Plc)
94
Croda International Plc distearoyl sn glycero 3 phospho
dga t2 mutant embryos are still capable of triacylglycerol synthesis in the YSL. A , TopFluor <t>C11</t> fatty acid was mixed with canola oil and microinjected into the yolk mass of dgat2 sa13945 mutants and siblings at 3 dpf. B , representative image of the TopFluor C11 signal in a dgat2 sa13945 zebrafish yolk sac 4 h after injection; scale = 200 μm. C and D , lipids were extracted from TopFluor C11 injected embryos and separated by thin layer chromatography (20 pooled embryos per sample, wild-type and dgat2 sa13945/+ embryos were combined). C , lipid classes were identified using TopFluor-labeled lipid standards. Arrow indicates TAG synthesized from TopFlour C11 in vivo . (CE: cholesterol ester, TAG: triacylglycerol, DAG: diacylglycerol, MAG: monoacylglycerol; PL: phospholipid; ∗ denote autofluorescent lipids in the fish lysate). D , the quantity of lipids in each class was quantified and expressed as a percent of total fluorescent lipids; N = 6 experiments, mean ± SD, Two-way ANOVA, not significantly different, p = 0.9929. Note, it appears that the TopFluor C11 is unable to be incorporated into cholesterol esters. E , large lipid droplets were manually dissected out of dgat2 sa13945 larvae at 6 dpf (40–50 LDs from 30 – 40 larvae). Lipids in LDs or an equivalent volume of media surrounding dissected larvae were separated by multistep gradient HPLC and detected with a charged aerosol detector (CAD) in picoamperes (pA) as described in . The lipid droplet samples contain large quantities of triacylglycerols and cholesterol esters. Traces are representative of three independent experiments.
Distearoyl Sn Glycero 3 Phospho, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glycerol/16%3A0-18%3A1(n9)+PG+-+IsoPure/bio_rxiv__2024__07__05__602135-118-19-37
Average 94 stars, based on 1 article reviews
distearoyl sn glycero 3 phospho - by Bioz Stars, 2026-09
94/100 stars
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1 stearoyl 2 arachidonoyl sn glycerol  (Croda International Plc)
94
Croda International Plc 1 stearoyl 2 arachidonoyl sn glycerol
dga t2 mutant embryos are still capable of triacylglycerol synthesis in the YSL. A , TopFluor <t>C11</t> fatty acid was mixed with canola oil and microinjected into the yolk mass of dgat2 sa13945 mutants and siblings at 3 dpf. B , representative image of the TopFluor C11 signal in a dgat2 sa13945 zebrafish yolk sac 4 h after injection; scale = 200 μm. C and D , lipids were extracted from TopFluor C11 injected embryos and separated by thin layer chromatography (20 pooled embryos per sample, wild-type and dgat2 sa13945/+ embryos were combined). C , lipid classes were identified using TopFluor-labeled lipid standards. Arrow indicates TAG synthesized from TopFlour C11 in vivo . (CE: cholesterol ester, TAG: triacylglycerol, DAG: diacylglycerol, MAG: monoacylglycerol; PL: phospholipid; ∗ denote autofluorescent lipids in the fish lysate). D , the quantity of lipids in each class was quantified and expressed as a percent of total fluorescent lipids; N = 6 experiments, mean ± SD, Two-way ANOVA, not significantly different, p = 0.9929. Note, it appears that the TopFluor C11 is unable to be incorporated into cholesterol esters. E , large lipid droplets were manually dissected out of dgat2 sa13945 larvae at 6 dpf (40–50 LDs from 30 – 40 larvae). Lipids in LDs or an equivalent volume of media surrounding dissected larvae were separated by multistep gradient HPLC and detected with a charged aerosol detector (CAD) in picoamperes (pA) as described in . The lipid droplet samples contain large quantities of triacylglycerols and cholesterol esters. Traces are representative of three independent experiments.
1 Stearoyl 2 Arachidonoyl Sn Glycerol, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glycerol/18%3A0-20%3A4+DG/pmc02573952-36-25-43
Average 94 stars, based on 1 article reviews
1 stearoyl 2 arachidonoyl sn glycerol - by Bioz Stars, 2026-09
94/100 stars
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1 2 diheptadecanoyl sn glycero 3 phospho 1 rac glycerol  (Croda International Plc)
94
Croda International Plc 1 2 diheptadecanoyl sn glycero 3 phospho 1 rac glycerol
dga t2 mutant embryos are still capable of triacylglycerol synthesis in the YSL. A , TopFluor <t>C11</t> fatty acid was mixed with canola oil and microinjected into the yolk mass of dgat2 sa13945 mutants and siblings at 3 dpf. B , representative image of the TopFluor C11 signal in a dgat2 sa13945 zebrafish yolk sac 4 h after injection; scale = 200 μm. C and D , lipids were extracted from TopFluor C11 injected embryos and separated by thin layer chromatography (20 pooled embryos per sample, wild-type and dgat2 sa13945/+ embryos were combined). C , lipid classes were identified using TopFluor-labeled lipid standards. Arrow indicates TAG synthesized from TopFlour C11 in vivo . (CE: cholesterol ester, TAG: triacylglycerol, DAG: diacylglycerol, MAG: monoacylglycerol; PL: phospholipid; ∗ denote autofluorescent lipids in the fish lysate). D , the quantity of lipids in each class was quantified and expressed as a percent of total fluorescent lipids; N = 6 experiments, mean ± SD, Two-way ANOVA, not significantly different, p = 0.9929. Note, it appears that the TopFluor C11 is unable to be incorporated into cholesterol esters. E , large lipid droplets were manually dissected out of dgat2 sa13945 larvae at 6 dpf (40–50 LDs from 30 – 40 larvae). Lipids in LDs or an equivalent volume of media surrounding dissected larvae were separated by multistep gradient HPLC and detected with a charged aerosol detector (CAD) in picoamperes (pA) as described in . The lipid droplet samples contain large quantities of triacylglycerols and cholesterol esters. Traces are representative of three independent experiments.
1 2 Diheptadecanoyl Sn Glycero 3 Phospho 1 Rac Glycerol, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glycerol/17%3A0+PG/pmc10199268-15-0-4
Average 94 stars, based on 1 article reviews
1 2 diheptadecanoyl sn glycero 3 phospho 1 rac glycerol - by Bioz Stars, 2026-09
94/100 stars
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1 9z octadecenoyl sn glycero 3 phosphoethanolamine  (Avanti Polar)
94
Avanti Polar 1 9z octadecenoyl sn glycero 3 phosphoethanolamine
dga t2 mutant embryos are still capable of triacylglycerol synthesis in the YSL. A , TopFluor <t>C11</t> fatty acid was mixed with canola oil and microinjected into the yolk mass of dgat2 sa13945 mutants and siblings at 3 dpf. B , representative image of the TopFluor C11 signal in a dgat2 sa13945 zebrafish yolk sac 4 h after injection; scale = 200 μm. C and D , lipids were extracted from TopFluor C11 injected embryos and separated by thin layer chromatography (20 pooled embryos per sample, wild-type and dgat2 sa13945/+ embryos were combined). C , lipid classes were identified using TopFluor-labeled lipid standards. Arrow indicates TAG synthesized from TopFlour C11 in vivo . (CE: cholesterol ester, TAG: triacylglycerol, DAG: diacylglycerol, MAG: monoacylglycerol; PL: phospholipid; ∗ denote autofluorescent lipids in the fish lysate). D , the quantity of lipids in each class was quantified and expressed as a percent of total fluorescent lipids; N = 6 experiments, mean ± SD, Two-way ANOVA, not significantly different, p = 0.9929. Note, it appears that the TopFluor C11 is unable to be incorporated into cholesterol esters. E , large lipid droplets were manually dissected out of dgat2 sa13945 larvae at 6 dpf (40–50 LDs from 30 – 40 larvae). Lipids in LDs or an equivalent volume of media surrounding dissected larvae were separated by multistep gradient HPLC and detected with a charged aerosol detector (CAD) in picoamperes (pA) as described in . The lipid droplet samples contain large quantities of triacylglycerols and cholesterol esters. Traces are representative of three independent experiments.
1 9z Octadecenoyl Sn Glycero 3 Phosphoethanolamine, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glycerol/18%3A0-18%3A2+DG/pm32634666-116-21-31
Average 94 stars, based on 1 article reviews
1 9z octadecenoyl sn glycero 3 phosphoethanolamine - by Bioz Stars, 2026-09
94/100 stars
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Image Search Results


Journal: Cell Reports Medicine

Article Title: Circadian organization of lipid landscape is perturbed in type 2 diabetic patients

doi: 10.1016/j.xcrm.2023.101299

Figure Lengend Snippet:

Article Snippet: Synthetic lipid standards [PC 12:0/12:0 (850335), PE 17:0/14:1 (LM-1104), PI 17:0/14:1 (LM-1504), PS 17:0/14:1 (LM-1304), Cer d18:1/17:0 (860517), SM d18:1/12:0 (860583), HexCer d18:1/8:0 (860540), DoxDHCer m18:0/12:0 (860460), 17:0-17:1-17:0 D5 TG (860903), 1,3-17:0 D5 DG (800854) and N-C12-deoxysphingosine m18:1/12:0 (860455)] were from Avanti Polar Lipids Inc. MTBE (methyl- tert -butyl ether) and methylamine (33% in absolute ethanol) were purchased from Sigma Aldrich.

Techniques: Clinical Proteomics, Recombinant, Sequencing, Expressing, Software, Mass Spectrometry

dga t2 mutant embryos are still capable of triacylglycerol synthesis in the YSL. A , TopFluor C11 fatty acid was mixed with canola oil and microinjected into the yolk mass of dgat2 sa13945 mutants and siblings at 3 dpf. B , representative image of the TopFluor C11 signal in a dgat2 sa13945 zebrafish yolk sac 4 h after injection; scale = 200 μm. C and D , lipids were extracted from TopFluor C11 injected embryos and separated by thin layer chromatography (20 pooled embryos per sample, wild-type and dgat2 sa13945/+ embryos were combined). C , lipid classes were identified using TopFluor-labeled lipid standards. Arrow indicates TAG synthesized from TopFlour C11 in vivo . (CE: cholesterol ester, TAG: triacylglycerol, DAG: diacylglycerol, MAG: monoacylglycerol; PL: phospholipid; ∗ denote autofluorescent lipids in the fish lysate). D , the quantity of lipids in each class was quantified and expressed as a percent of total fluorescent lipids; N = 6 experiments, mean ± SD, Two-way ANOVA, not significantly different, p = 0.9929. Note, it appears that the TopFluor C11 is unable to be incorporated into cholesterol esters. E , large lipid droplets were manually dissected out of dgat2 sa13945 larvae at 6 dpf (40–50 LDs from 30 – 40 larvae). Lipids in LDs or an equivalent volume of media surrounding dissected larvae were separated by multistep gradient HPLC and detected with a charged aerosol detector (CAD) in picoamperes (pA) as described in . The lipid droplet samples contain large quantities of triacylglycerols and cholesterol esters. Traces are representative of three independent experiments.

Journal: The Journal of Biological Chemistry

Article Title: Zebrafish are resilient to the loss of major diacylglycerol acyltransferase enzymes

doi: 10.1016/j.jbc.2024.107973

Figure Lengend Snippet: dga t2 mutant embryos are still capable of triacylglycerol synthesis in the YSL. A , TopFluor C11 fatty acid was mixed with canola oil and microinjected into the yolk mass of dgat2 sa13945 mutants and siblings at 3 dpf. B , representative image of the TopFluor C11 signal in a dgat2 sa13945 zebrafish yolk sac 4 h after injection; scale = 200 μm. C and D , lipids were extracted from TopFluor C11 injected embryos and separated by thin layer chromatography (20 pooled embryos per sample, wild-type and dgat2 sa13945/+ embryos were combined). C , lipid classes were identified using TopFluor-labeled lipid standards. Arrow indicates TAG synthesized from TopFlour C11 in vivo . (CE: cholesterol ester, TAG: triacylglycerol, DAG: diacylglycerol, MAG: monoacylglycerol; PL: phospholipid; ∗ denote autofluorescent lipids in the fish lysate). D , the quantity of lipids in each class was quantified and expressed as a percent of total fluorescent lipids; N = 6 experiments, mean ± SD, Two-way ANOVA, not significantly different, p = 0.9929. Note, it appears that the TopFluor C11 is unable to be incorporated into cholesterol esters. E , large lipid droplets were manually dissected out of dgat2 sa13945 larvae at 6 dpf (40–50 LDs from 30 – 40 larvae). Lipids in LDs or an equivalent volume of media surrounding dissected larvae were separated by multistep gradient HPLC and detected with a charged aerosol detector (CAD) in picoamperes (pA) as described in . The lipid droplet samples contain large quantities of triacylglycerols and cholesterol esters. Traces are representative of three independent experiments.

Article Snippet: TopFluor-labeled standards for TAG, CE, PC, DG, MG and C11 (TopFluor TAG: 810298; TopFluor CE: 810290; TopFluor PC: 810,281 TopFluor DG: 810300; MG: 810296; TopFluor C11 custom synthesis, Avanti Polar Lipids) were added to lipids extracted from uninjected embryos and loaded similarly onto the TLC plate.

Techniques: Mutagenesis, Injection, Thin Layer Chromatography, Labeling, Synthesized, In Vivo, Aerosol