glosensor 22 f Search Results


glosensor-22f  (Promega)
90
Promega glosensor-22f
Glosensor 22f, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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camp biosensor glosensor-22f  (Promega)
90
Promega camp biosensor glosensor-22f
Camp Biosensor Glosensor 22f, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glosensor-22f luminescent camp-sensing protein  (Promega)
90
Promega glosensor-22f luminescent camp-sensing protein
Glosensor 22f Luminescent Camp Sensing Protein, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glosensor-22f camp  (Promega)
90
Promega glosensor-22f camp
Glosensor 22f Camp, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glosensor-22f camp - by Bioz Stars, 2026-06
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glosensor 22-f  (Lonza)
90
Lonza glosensor 22-f
( A ) cAMP levels from 3T3-L1 adipocytes treated with or without 100 ng/ml TNFα for the indicated amount of time (left panel: 0–12 hr, right panel: 0–24 hr) followed by treatment with or without 25 μM FSK. **p<0.01. Performed in duplicate. ( B ) Relative gene expression (top panel: Ucp1 , bottom panel: Ikbke ) in 3T3-L1 adipocytes treated with or without 100 ng/ml TNFα for 24 hr followed by treatment with or without 10 μM ISO or 50 μM FSK, or 10 μM CL-316,243 for 4 hrs. ** P<0.01 and **p<0.0001. Performed in tripilicate. ( C ) cAMP levels as measured by <t>Glosensor</t> from 3T3-L1 adipocytes treated with or without 5 μg/ml poly (I:C) for 24 hr followed by treatment with or without 50 μM FSK over the course of 75 min. Performed in triplicate. ( D ) Glycerol release from 3T3-L1 adipocytes treated with or without different concentrations of poly (I:C) as indicated for 24 hr followed by treatment with or without 10 μM ISO or 50 μM FSK. ** p<0.0001. Performed in quadruplicate. ( E ) Immunoblots of whole cell lysates from 3T3-L1 adipocytes treated with or without different concentrations of poly (I:C) as same as for 24 hr followed by treatment with or without 10 μM ISO or 50 μM FSK. Results were replicated in multiple experiments. DOI: http://dx.doi.org/10.7554/eLife.01119.006
Glosensor 22 F, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glosensor 22-f - by Bioz Stars, 2026-06
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luciferase-based intracellular camp probe glosensor 22 f  (Promega)
90
Promega luciferase-based intracellular camp probe glosensor 22 f
( A ) cAMP levels from 3T3-L1 adipocytes treated with or without 100 ng/ml TNFα for the indicated amount of time (left panel: 0–12 hr, right panel: 0–24 hr) followed by treatment with or without 25 μM FSK. **p<0.01. Performed in duplicate. ( B ) Relative gene expression (top panel: Ucp1 , bottom panel: Ikbke ) in 3T3-L1 adipocytes treated with or without 100 ng/ml TNFα for 24 hr followed by treatment with or without 10 μM ISO or 50 μM FSK, or 10 μM CL-316,243 for 4 hrs. ** P<0.01 and **p<0.0001. Performed in tripilicate. ( C ) cAMP levels as measured by <t>Glosensor</t> from 3T3-L1 adipocytes treated with or without 5 μg/ml poly (I:C) for 24 hr followed by treatment with or without 50 μM FSK over the course of 75 min. Performed in triplicate. ( D ) Glycerol release from 3T3-L1 adipocytes treated with or without different concentrations of poly (I:C) as indicated for 24 hr followed by treatment with or without 10 μM ISO or 50 μM FSK. ** p<0.0001. Performed in quadruplicate. ( E ) Immunoblots of whole cell lysates from 3T3-L1 adipocytes treated with or without different concentrations of poly (I:C) as same as for 24 hr followed by treatment with or without 10 μM ISO or 50 μM FSK. Results were replicated in multiple experiments. DOI: http://dx.doi.org/10.7554/eLife.01119.006
Luciferase Based Intracellular Camp Probe Glosensor 22 F, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid encoding the luciferase-based intracellular camp probe glosensor 22 f  (Promega)
90
Promega plasmid encoding the luciferase-based intracellular camp probe glosensor 22 f
( A ) cAMP levels from 3T3-L1 adipocytes treated with or without 100 ng/ml TNFα for the indicated amount of time (left panel: 0–12 hr, right panel: 0–24 hr) followed by treatment with or without 25 μM FSK. **p<0.01. Performed in duplicate. ( B ) Relative gene expression (top panel: Ucp1 , bottom panel: Ikbke ) in 3T3-L1 adipocytes treated with or without 100 ng/ml TNFα for 24 hr followed by treatment with or without 10 μM ISO or 50 μM FSK, or 10 μM CL-316,243 for 4 hrs. ** P<0.01 and **p<0.0001. Performed in tripilicate. ( C ) cAMP levels as measured by <t>Glosensor</t> from 3T3-L1 adipocytes treated with or without 5 μg/ml poly (I:C) for 24 hr followed by treatment with or without 50 μM FSK over the course of 75 min. Performed in triplicate. ( D ) Glycerol release from 3T3-L1 adipocytes treated with or without different concentrations of poly (I:C) as indicated for 24 hr followed by treatment with or without 10 μM ISO or 50 μM FSK. ** p<0.0001. Performed in quadruplicate. ( E ) Immunoblots of whole cell lysates from 3T3-L1 adipocytes treated with or without different concentrations of poly (I:C) as same as for 24 hr followed by treatment with or without 10 μM ISO or 50 μM FSK. Results were replicated in multiple experiments. DOI: http://dx.doi.org/10.7554/eLife.01119.006
Plasmid Encoding The Luciferase Based Intracellular Camp Probe Glosensor 22 F, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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luciferase-based pglosensor-22f camp reporter plasmid glosensor  (Promega)
90
Promega luciferase-based pglosensor-22f camp reporter plasmid glosensor
( A ) cAMP levels from 3T3-L1 adipocytes treated with or without 100 ng/ml TNFα for the indicated amount of time (left panel: 0–12 hr, right panel: 0–24 hr) followed by treatment with or without 25 μM FSK. **p<0.01. Performed in duplicate. ( B ) Relative gene expression (top panel: Ucp1 , bottom panel: Ikbke ) in 3T3-L1 adipocytes treated with or without 100 ng/ml TNFα for 24 hr followed by treatment with or without 10 μM ISO or 50 μM FSK, or 10 μM CL-316,243 for 4 hrs. ** P<0.01 and **p<0.0001. Performed in tripilicate. ( C ) cAMP levels as measured by <t>Glosensor</t> from 3T3-L1 adipocytes treated with or without 5 μg/ml poly (I:C) for 24 hr followed by treatment with or without 50 μM FSK over the course of 75 min. Performed in triplicate. ( D ) Glycerol release from 3T3-L1 adipocytes treated with or without different concentrations of poly (I:C) as indicated for 24 hr followed by treatment with or without 10 μM ISO or 50 μM FSK. ** p<0.0001. Performed in quadruplicate. ( E ) Immunoblots of whole cell lysates from 3T3-L1 adipocytes treated with or without different concentrations of poly (I:C) as same as for 24 hr followed by treatment with or without 10 μM ISO or 50 μM FSK. Results were replicated in multiple experiments. DOI: http://dx.doi.org/10.7554/eLife.01119.006
Luciferase Based Pglosensor 22f Camp Reporter Plasmid Glosensor, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/luciferase-based pglosensor-22f camp reporter plasmid glosensor/product/Promega
Average 90 stars, based on 1 article reviews
luciferase-based pglosensor-22f camp reporter plasmid glosensor - by Bioz Stars, 2026-06
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glosensor-22f camp gene  (Promega)
90
Promega glosensor-22f camp gene
( A ) cAMP levels from 3T3-L1 adipocytes treated with or without 100 ng/ml TNFα for the indicated amount of time (left panel: 0–12 hr, right panel: 0–24 hr) followed by treatment with or without 25 μM FSK. **p<0.01. Performed in duplicate. ( B ) Relative gene expression (top panel: Ucp1 , bottom panel: Ikbke ) in 3T3-L1 adipocytes treated with or without 100 ng/ml TNFα for 24 hr followed by treatment with or without 10 μM ISO or 50 μM FSK, or 10 μM CL-316,243 for 4 hrs. ** P<0.01 and **p<0.0001. Performed in tripilicate. ( C ) cAMP levels as measured by <t>Glosensor</t> from 3T3-L1 adipocytes treated with or without 5 μg/ml poly (I:C) for 24 hr followed by treatment with or without 50 μM FSK over the course of 75 min. Performed in triplicate. ( D ) Glycerol release from 3T3-L1 adipocytes treated with or without different concentrations of poly (I:C) as indicated for 24 hr followed by treatment with or without 10 μM ISO or 50 μM FSK. ** p<0.0001. Performed in quadruplicate. ( E ) Immunoblots of whole cell lysates from 3T3-L1 adipocytes treated with or without different concentrations of poly (I:C) as same as for 24 hr followed by treatment with or without 10 μM ISO or 50 μM FSK. Results were replicated in multiple experiments. DOI: http://dx.doi.org/10.7554/eLife.01119.006
Glosensor 22f Camp Gene, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
glosensor-22f camp gene - by Bioz Stars, 2026-06
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camp-dependent firefly luciferase glosensor-22f  (Promega)
90
Promega camp-dependent firefly luciferase glosensor-22f
( A ) cAMP levels from 3T3-L1 adipocytes treated with or without 100 ng/ml TNFα for the indicated amount of time (left panel: 0–12 hr, right panel: 0–24 hr) followed by treatment with or without 25 μM FSK. **p<0.01. Performed in duplicate. ( B ) Relative gene expression (top panel: Ucp1 , bottom panel: Ikbke ) in 3T3-L1 adipocytes treated with or without 100 ng/ml TNFα for 24 hr followed by treatment with or without 10 μM ISO or 50 μM FSK, or 10 μM CL-316,243 for 4 hrs. ** P<0.01 and **p<0.0001. Performed in tripilicate. ( C ) cAMP levels as measured by <t>Glosensor</t> from 3T3-L1 adipocytes treated with or without 5 μg/ml poly (I:C) for 24 hr followed by treatment with or without 50 μM FSK over the course of 75 min. Performed in triplicate. ( D ) Glycerol release from 3T3-L1 adipocytes treated with or without different concentrations of poly (I:C) as indicated for 24 hr followed by treatment with or without 10 μM ISO or 50 μM FSK. ** p<0.0001. Performed in quadruplicate. ( E ) Immunoblots of whole cell lysates from 3T3-L1 adipocytes treated with or without different concentrations of poly (I:C) as same as for 24 hr followed by treatment with or without 10 μM ISO or 50 μM FSK. Results were replicated in multiple experiments. DOI: http://dx.doi.org/10.7554/eLife.01119.006
Camp Dependent Firefly Luciferase Glosensor 22f, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/camp-dependent firefly luciferase glosensor-22f/product/Promega
Average 90 stars, based on 1 article reviews
camp-dependent firefly luciferase glosensor-22f - by Bioz Stars, 2026-06
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bioluminescent camp reporter glosensor 22 f  (Promega)
90
Promega bioluminescent camp reporter glosensor 22 f
( A ) cAMP levels from 3T3-L1 adipocytes treated with or without 100 ng/ml TNFα for the indicated amount of time (left panel: 0–12 hr, right panel: 0–24 hr) followed by treatment with or without 25 μM FSK. **p<0.01. Performed in duplicate. ( B ) Relative gene expression (top panel: Ucp1 , bottom panel: Ikbke ) in 3T3-L1 adipocytes treated with or without 100 ng/ml TNFα for 24 hr followed by treatment with or without 10 μM ISO or 50 μM FSK, or 10 μM CL-316,243 for 4 hrs. ** P<0.01 and **p<0.0001. Performed in tripilicate. ( C ) cAMP levels as measured by <t>Glosensor</t> from 3T3-L1 adipocytes treated with or without 5 μg/ml poly (I:C) for 24 hr followed by treatment with or without 50 μM FSK over the course of 75 min. Performed in triplicate. ( D ) Glycerol release from 3T3-L1 adipocytes treated with or without different concentrations of poly (I:C) as indicated for 24 hr followed by treatment with or without 10 μM ISO or 50 μM FSK. ** p<0.0001. Performed in quadruplicate. ( E ) Immunoblots of whole cell lysates from 3T3-L1 adipocytes treated with or without different concentrations of poly (I:C) as same as for 24 hr followed by treatment with or without 10 μM ISO or 50 μM FSK. Results were replicated in multiple experiments. DOI: http://dx.doi.org/10.7554/eLife.01119.006
Bioluminescent Camp Reporter Glosensor 22 F, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
bioluminescent camp reporter glosensor 22 f - by Bioz Stars, 2026-06
90/100 stars
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glosensor 22f camp sensor  (Promega)
90
Promega glosensor 22f camp sensor
( A ) Confocal images showing cell surface expression in mammalian HEK293 cells of 5-HT receptors cloned from S. mansoni ( Sm .5HTR L ), S. haematobium ( Sh. 5HTR) and S. japonicum ( Sj .5HTR) localized by COOH-terminally tagged eGFP. Scalebar, 50 µm. ( B ) Schematic of luminescent cAMP sensor bioassay. cAMP generated by schistosome 5-HTRs (blue) binds the engineered <t>GloSensor</t> luciferase, switching the sensor to a more active conformation resulting in enhanced luminescence output. ( C ) Kinetics of signal following the addition of 5-HT (1 µM) to HEK293 cells co-transfected with luminescent cAMP sensor and individual schistosome 5-HT receptors. Open circles, cells not transfected with 5-HT receptor, colored circles represent measurements in cells transfected with Sm .5HTR L (black), ( Sh. 5HTR (blue) and Sj .5HTR (red). ( D ) Serotonin dose-response curves for each of the three receptors (colored circles) and HEK293 cells expressing the cAMP sensor alone (open circles). Data reflect mean ± standard error of at least 3 biological replicates.
Glosensor 22f Camp Sensor, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/glosensor 22f camp sensor/product/Promega
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glosensor 22f camp sensor - by Bioz Stars, 2026-06
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Image Search Results


( A ) cAMP levels from 3T3-L1 adipocytes treated with or without 100 ng/ml TNFα for the indicated amount of time (left panel: 0–12 hr, right panel: 0–24 hr) followed by treatment with or without 25 μM FSK. **p<0.01. Performed in duplicate. ( B ) Relative gene expression (top panel: Ucp1 , bottom panel: Ikbke ) in 3T3-L1 adipocytes treated with or without 100 ng/ml TNFα for 24 hr followed by treatment with or without 10 μM ISO or 50 μM FSK, or 10 μM CL-316,243 for 4 hrs. ** P<0.01 and **p<0.0001. Performed in tripilicate. ( C ) cAMP levels as measured by Glosensor from 3T3-L1 adipocytes treated with or without 5 μg/ml poly (I:C) for 24 hr followed by treatment with or without 50 μM FSK over the course of 75 min. Performed in triplicate. ( D ) Glycerol release from 3T3-L1 adipocytes treated with or without different concentrations of poly (I:C) as indicated for 24 hr followed by treatment with or without 10 μM ISO or 50 μM FSK. ** p<0.0001. Performed in quadruplicate. ( E ) Immunoblots of whole cell lysates from 3T3-L1 adipocytes treated with or without different concentrations of poly (I:C) as same as for 24 hr followed by treatment with or without 10 μM ISO or 50 μM FSK. Results were replicated in multiple experiments. DOI: http://dx.doi.org/10.7554/eLife.01119.006

Journal: eLife

Article Title: Inflammation produces catecholamine resistance in obesity via activation of PDE3B by the protein kinases IKKε and TBK1

doi: 10.7554/eLife.01119

Figure Lengend Snippet: ( A ) cAMP levels from 3T3-L1 adipocytes treated with or without 100 ng/ml TNFα for the indicated amount of time (left panel: 0–12 hr, right panel: 0–24 hr) followed by treatment with or without 25 μM FSK. **p<0.01. Performed in duplicate. ( B ) Relative gene expression (top panel: Ucp1 , bottom panel: Ikbke ) in 3T3-L1 adipocytes treated with or without 100 ng/ml TNFα for 24 hr followed by treatment with or without 10 μM ISO or 50 μM FSK, or 10 μM CL-316,243 for 4 hrs. ** P<0.01 and **p<0.0001. Performed in tripilicate. ( C ) cAMP levels as measured by Glosensor from 3T3-L1 adipocytes treated with or without 5 μg/ml poly (I:C) for 24 hr followed by treatment with or without 50 μM FSK over the course of 75 min. Performed in triplicate. ( D ) Glycerol release from 3T3-L1 adipocytes treated with or without different concentrations of poly (I:C) as indicated for 24 hr followed by treatment with or without 10 μM ISO or 50 μM FSK. ** p<0.0001. Performed in quadruplicate. ( E ) Immunoblots of whole cell lysates from 3T3-L1 adipocytes treated with or without different concentrations of poly (I:C) as same as for 24 hr followed by treatment with or without 10 μM ISO or 50 μM FSK. Results were replicated in multiple experiments. DOI: http://dx.doi.org/10.7554/eLife.01119.006

Article Snippet: 80 μl of packed 3T3-L1 cells was electroporated with 3 μg of Glosensor 22-F using Amaxa Cell Line Nucleofector Kit L (Lonza) according to the manufacturer’s protocol.

Techniques: Gene Expression, Western Blot

( A ) Confocal images showing cell surface expression in mammalian HEK293 cells of 5-HT receptors cloned from S. mansoni ( Sm .5HTR L ), S. haematobium ( Sh. 5HTR) and S. japonicum ( Sj .5HTR) localized by COOH-terminally tagged eGFP. Scalebar, 50 µm. ( B ) Schematic of luminescent cAMP sensor bioassay. cAMP generated by schistosome 5-HTRs (blue) binds the engineered GloSensor luciferase, switching the sensor to a more active conformation resulting in enhanced luminescence output. ( C ) Kinetics of signal following the addition of 5-HT (1 µM) to HEK293 cells co-transfected with luminescent cAMP sensor and individual schistosome 5-HT receptors. Open circles, cells not transfected with 5-HT receptor, colored circles represent measurements in cells transfected with Sm .5HTR L (black), ( Sh. 5HTR (blue) and Sj .5HTR (red). ( D ) Serotonin dose-response curves for each of the three receptors (colored circles) and HEK293 cells expressing the cAMP sensor alone (open circles). Data reflect mean ± standard error of at least 3 biological replicates.

Journal: eLife

Article Title: Coalescing beneficial host and deleterious antiparasitic actions as an antischistosomal strategy

doi: 10.7554/eLife.35755

Figure Lengend Snippet: ( A ) Confocal images showing cell surface expression in mammalian HEK293 cells of 5-HT receptors cloned from S. mansoni ( Sm .5HTR L ), S. haematobium ( Sh. 5HTR) and S. japonicum ( Sj .5HTR) localized by COOH-terminally tagged eGFP. Scalebar, 50 µm. ( B ) Schematic of luminescent cAMP sensor bioassay. cAMP generated by schistosome 5-HTRs (blue) binds the engineered GloSensor luciferase, switching the sensor to a more active conformation resulting in enhanced luminescence output. ( C ) Kinetics of signal following the addition of 5-HT (1 µM) to HEK293 cells co-transfected with luminescent cAMP sensor and individual schistosome 5-HT receptors. Open circles, cells not transfected with 5-HT receptor, colored circles represent measurements in cells transfected with Sm .5HTR L (black), ( Sh. 5HTR (blue) and Sj .5HTR (red). ( D ) Serotonin dose-response curves for each of the three receptors (colored circles) and HEK293 cells expressing the cAMP sensor alone (open circles). Data reflect mean ± standard error of at least 3 biological replicates.

Article Snippet: Commercial assay or kit , GloSensor 22F cAMP sensor , Company , Promega; E2301 , .

Techniques: Expressing, Clone Assay, Bioassay, Generated, Luciferase, Transfection

Journal: eLife

Article Title: Coalescing beneficial host and deleterious antiparasitic actions as an antischistosomal strategy

doi: 10.7554/eLife.35755

Figure Lengend Snippet:

Article Snippet: Commercial assay or kit , GloSensor 22F cAMP sensor , Company , Promega; E2301 , .

Techniques: Recombinant, Software