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Article Title: Detection of Adulteration of Notoginseng Root Extract with Other Panax Species by Quantitative HPLC Coupled with PCA

doi: 10.1021/jf803320d

Figure Lengend Snippet: Typical chromatograms of extracts from (A) the root and (B) leaf of Panax notoginseng; (C) the root and (D) leaf of Panax ginseng; and (E) the root and (F) leaf of Panax quinquefolius; a mixture of Panax notoginseng root with (G) Panax ginseng root and (H) leaf. Saponin peaks: notoginsenoside R1 (1), and ginsenosides Rg1 (2), Re (3), Rh1 (4), Rg2 (5), 20R-Rg2 (6), Rb1 (7), Rc (8), Rb2 (9), Rb3 (10), Rd (11), and Rg3 (12).

Article Snippet: Standards for ginsenosides Rb1, Rb2, Rc, Rd, Re, and Rg1 were obtained from Indofine Chemical Company (Somerville, NJ); ginsenosides Rb3, Rg2, 20 R -Rg2, Rg3, Rh1, and notoginsenoside R1 were obtained from the Delta Information Center for Natural Organic Compounds (Xuancheng, Anhui, China).

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Journal:

Article Title: Detection of Adulteration of Notoginseng Root Extract with Other Panax Species by Quantitative HPLC Coupled with PCA

doi: 10.1021/jf803320d

Figure Lengend Snippet: Typical Saponin Content in Panax Extracts (mg/g)

Article Snippet: Standards for ginsenosides Rb1, Rb2, Rc, Rd, Re, and Rg1 were obtained from Indofine Chemical Company (Somerville, NJ); ginsenosides Rb3, Rg2, 20 R -Rg2, Rg3, Rh1, and notoginsenoside R1 were obtained from the Delta Information Center for Natural Organic Compounds (Xuancheng, Anhui, China).

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Journal:

Article Title: Detection of Adulteration of Notoginseng Root Extract with Other Panax Species by Quantitative HPLC Coupled with PCA

doi: 10.1021/jf803320d

Figure Lengend Snippet: Principal component analysis (PCA) plots with (A) principal components 1 and 2 and (B) principal components 1 and 3, using contents of 12 saponins as input data. The 12 saponins are notoginsenoside R1, ginsenosides Rg1, Re, Rh1, Rg2, 20R-Rg2, Rb1, Rc, Rb2, Rb3, Rd and Rg3. The group numbers of Panax extracts are listed in Table 1.

Article Snippet: Standards for ginsenosides Rb1, Rb2, Rc, Rd, Re, and Rg1 were obtained from Indofine Chemical Company (Somerville, NJ); ginsenosides Rb3, Rg2, 20 R -Rg2, Rg3, Rh1, and notoginsenoside R1 were obtained from the Delta Information Center for Natural Organic Compounds (Xuancheng, Anhui, China).

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Journal:

Article Title: Detection of Adulteration of Notoginseng Root Extract with Other Panax Species by Quantitative HPLC Coupled with PCA

doi: 10.1021/jf803320d

Figure Lengend Snippet: Principal component analysis (PCA) plots with (A) principal components 1 and 2 and (B) principal components 1 and 3, using contents of 8 saponins as input data. The 8 saponins are notoginsenoside R1, ginsenosides Rg1, Re, Rb1, Rc, Rb2, Rb3 and Rd. The group numbers of Panax extracts are listed in Table 1. Arrows indicate groups 7, 8 and 9 were extracted from mixed plant parts.

Article Snippet: Standards for ginsenosides Rb1, Rb2, Rc, Rd, Re, and Rg1 were obtained from Indofine Chemical Company (Somerville, NJ); ginsenosides Rb3, Rg2, 20 R -Rg2, Rg3, Rh1, and notoginsenoside R1 were obtained from the Delta Information Center for Natural Organic Compounds (Xuancheng, Anhui, China).

Techniques:

Journal: Cancers

Article Title: A Panax quinquefolius -Based Preparation Prevents the Impact of 5-FU on Activity/Exploration Behaviors and Not on Cognitive Functions Mitigating Gut Microbiota and Inflammation in Mice

doi: 10.3390/cancers14184403

Figure Lengend Snippet: Detection of ginsenosides and protopanaxadiol in sera of placebo and 5-FU mice treated with Qiseng ® . ( A ) Schematic illustration showing the timeline for treatments and time of serum collection in mice to study the composition in ginsenosides and potential metabolites. C57BL/6J Rj mice received i.p. 5-FU injections once a week for 3 weeks ( arrow ) and per os placebo, vitamin C or Qiseng ® once a day, 5 times a week for 2 weeks. Mice received four BrdU i.p. injections (once a day) at W2 and W3; then, serum and cecum were collected for analysis. ( B ) HPLC chromatogram and chemoprofile of Qiseng ® and some ginsenosides from a 1/10 Qiseng diluted solution ( Left ) and from a representative example of a NaCl/Qiseng ® -treated mouse serum ( Right ). ( C ) Relative abundance of Rb1, Rc, Rd, Re, Rg5/Rg6/Rk1, Rg3/F2/RG4/Rf4, Ro, Rg1, F11/Rf ginsenosides in sera of NaCl/Qiseng ® and 5-FU/Qiseng ® -treated mice. The Rb1, Rd and Rc ginsenosides were predominantly found in sera of both groups, and the Rc ginsenosides proportion was significantly higher in 5-FU/Qiseng ® mice. ( D ) Schematic illustration of the composition of Qiseng ® and the potential metabolization by gut microbiota into protopanatriol, protopanaxadiol and Factor K metabolites ( Left ). Sole protopanaxadiol can be detected in only some serum collected from NaCl/Qiseng ® and 5-FU/Qiseng ® mice. ( E ) Percentage of ginsenosides ( Left ) or Protopanaxadiol ( Right ) in sera of NaCl/Qiseng® and 5-FU/Qiseng® mice. Data are represented as box plots with the median or bar plots of mean ± SEM with symbols of individual data points. In each analysis, NaCl/Qiseng ® ( n = 4–7), 5-FU/Qiseng ® ( n = 4–6). Mann–Whitney; * p < 0.05, ns: not significant). 5-FU: 5-fluorouracil, BrdU: 5-bromo-2′-deoxyuridine, D: Day, W: Week, i.p.: intraperitoneal injection.

Article Snippet: Ginsenoside Rb1 was purchased from Sigma (Saint-Louis, MO, USA), and Ginsenosides Rg1, Re, Rc, Rd, K, Protopanaxadiol, Protopanaxatriol standards were purchased from Extrasynthese (Genay, France).

Techniques: MANN-WHITNEY, Injection

Journal: Frontiers in Oncology

Article Title: Ginsenoside Rh7 Suppresses Proliferation, Migration and Invasion of NSCLC Cells Through Targeting ILF3-AS1 Mediated miR-212/SMAD1 Axis

doi: 10.3389/fonc.2021.656132

Figure Lengend Snippet: Effects of different concentrations of Rh7 on the survival rate of A549 and H1299 cells. (A, B) Effect of Rh7 on the survival rate of A549 and H1299 cells in the dose-dependent pattern. (C, D) Effect of Rh7 on the survival rate of A549 and H1299 cells in the time-dependent pattern.* P < 0.05, ** P < 0.01.

Article Snippet: Ginsenosides Rc, Rh1, Rh3 and Rh7 were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China), dissolved in dimethyl sulfoxide (DMSO), and then diluted to a concentration range of 0-100μM.

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Journal: Frontiers in Oncology

Article Title: Ginsenoside Rh7 Suppresses Proliferation, Migration and Invasion of NSCLC Cells Through Targeting ILF3-AS1 Mediated miR-212/SMAD1 Axis

doi: 10.3389/fonc.2021.656132

Figure Lengend Snippet: Rh7 treatment inhibits the migration and invasion ability of A549 and H1299 cells. (A, B) 25 μM Rh7 treatment inhibited the migration ability of the A549 cell line, and (C, D) 25 μM Rh7 treatment inhibited the migration ability of the H1299 cell line. (E, F) 25 μM Rh7 treatment inhibited the invasive ability of A549 cell line. (G, H) 25 μM Rh7 treatment inhibited the invasive ability of H1299 cell line. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Ginsenosides Rc, Rh1, Rh3 and Rh7 were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China), dissolved in dimethyl sulfoxide (DMSO), and then diluted to a concentration range of 0-100μM.

Techniques: Migration

Journal: Frontiers in Oncology

Article Title: Ginsenoside Rh7 Suppresses Proliferation, Migration and Invasion of NSCLC Cells Through Targeting ILF3-AS1 Mediated miR-212/SMAD1 Axis

doi: 10.3389/fonc.2021.656132

Figure Lengend Snippet: Bioinformatics analysis of the Rh7 regulated genes in NSCLC. (A) Heatmap diagram of the differentially expressed genes in H1299 cells is stimulated by ginsenoside Rh7. The abscissa represents genes and the ordinate represents sample names; red represents up-regulated expression and blue represents down-regulated expression; genes and samples are clustered separately. (B) GO analysis of differentially altered genes in H1299 cells stimulated by ginsenoside Rh7. (C) 496 clustering results. (D) The trend of scale independence when soft threshold is 1 to 20. (E) The trend of average connectivity when soft threshold is 1 to 20.

Article Snippet: Ginsenosides Rc, Rh1, Rh3 and Rh7 were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China), dissolved in dimethyl sulfoxide (DMSO), and then diluted to a concentration range of 0-100μM.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: Ginsenoside Rh7 Suppresses Proliferation, Migration and Invasion of NSCLC Cells Through Targeting ILF3-AS1 Mediated miR-212/SMAD1 Axis

doi: 10.3389/fonc.2021.656132

Figure Lengend Snippet: Knocking down of ILF3-AS1 reduced the growth rate of A549 and H1299 cells. (A, B) A549 and H1299 cell lines were treated with Rh7 to detect NCF1C, C7orf13, LOC147727, SNORA25, SNHG9, TP53TG1, RPPH1 and SNORA62 gene expression levels. (C, F) RT-PCR was used to detect ILF3-AS1 levels in non-small cell lung cancer cell lines A549 (C) and H1299 (E) . (D, F) Knockdown of ILF3-AS1 significantly suppressed cell proliferation in non-small cell lung cancer cell lines A549 (D) and H1299 (F) . (G, H) Knockdown of ILF3-AS1 could significantly block the cell cycle progression of H1299. * P < 0.05, ** P < 0.01.

Article Snippet: Ginsenosides Rc, Rh1, Rh3 and Rh7 were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China), dissolved in dimethyl sulfoxide (DMSO), and then diluted to a concentration range of 0-100μM.

Techniques: Gene Expression, Reverse Transcription Polymerase Chain Reaction, Knockdown, Blocking Assay

Journal: Frontiers in Oncology

Article Title: Ginsenoside Rh7 Suppresses Proliferation, Migration and Invasion of NSCLC Cells Through Targeting ILF3-AS1 Mediated miR-212/SMAD1 Axis

doi: 10.3389/fonc.2021.656132

Figure Lengend Snippet: Diagram of the mechanism of ginsenoside Rh7 targeting the ILF3-AS1/miR212/SAMD1 axis in NSCLC.

Article Snippet: Ginsenosides Rc, Rh1, Rh3 and Rh7 were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China), dissolved in dimethyl sulfoxide (DMSO), and then diluted to a concentration range of 0-100μM.

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