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Image Search Results
Journal: Biomedicines
Article Title: Disease-Associated Dopamine Receptor D2 Variants Exhibit Functional Consequences Depending on Different Heterotrimeric G-Protein Subunit Combinations
doi: 10.3390/biomedicines13010046
Figure Lengend Snippet: G-protein activation of the WT, I212F, and M345R. ( A ) Concentration–response curves for the WT, I212F, and M345R with Gα i/o , β-, and γ-subunits as indicated, following dopamine application. netBRET (GFP2/Rluc8) values are normalized to the mean of the max dopamine response of the WT same-day control and reported as a percentage of the WT same-day control (% of G-protein activation (WT E max )). ( B ) Basal activity for the WT, I212F, and M345R are plotted as percentages of the mean WT E max same-day control (Basal activity (% of WT Max)). ( C ) Comparison of pEC 50 values for the WT, I212F, and M345R. ( D ) WT, I212F, and M345R maximal efficacy expressed as a percentage of the mean of the maximum of the WT same-day control (E max (% of WT E max )). Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc test, or Student’s t -test as given in , and indicated as: * p < 0.05; ** p < 0.01; *** p < 0.001; ns—not significant; nd—not determined. Data shown as mean ± SEM.
Article Snippet: Gα i1 -RLuc8 (Addgene, #140973), Gα i2 -RLuc8 (Addgene, #140974), Gα i3 -RLuc8 (Addgene, #140975), Gα oA -RLuc8 (Addgene, #140976), Gα oB -RLuc8 (Addgene, #140977), Gβ 1 (Addgene, #140987), Gβ 2 (Addgene, #166772), Gβ 3 (Addgene, #140988), Gγ 3 -GFP2 (Addgene, #166775), Gγ 7 -GFP2 (Addgene, #166778),
Techniques: Activation Assay, Concentration Assay, Control, Activity Assay, Comparison
Journal: Biomedicines
Article Title: Disease-Associated Dopamine Receptor D2 Variants Exhibit Functional Consequences Depending on Different Heterotrimeric G-Protein Subunit Combinations
doi: 10.3390/biomedicines13010046
Figure Lengend Snippet: Most abundant expressed G-protein combination activation of the WT, I212F, and M345R. ( A ) Concentration–response curves for the WT, I212F, and M345R with Gα i/o , β-, and γ-subunits as indicated, following dopamine application. netBRET (GFP2/Rluc8) values are normalized to the mean of the max dopamine response of the WT same-day control and reported as a percentage of the WT same-day control (% of G-protein activation (WT E max )). ( B ) Basal activity for the WT, I212F, and M345R are plotted as percentages of the mean of the WT E max same-day control (Basal activity (% of WT Max)). ( C ) Comparison of pEC 50 values for the WT, I212F, and M345R. ( D ) WT, I212F, and M345R maximal efficacy expressed as a percentage of the mean maximum of the WT same-day control (E max (% of WT E max )). Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc test, or Student’s t -test as given in , and indicated as: * p < 0.05; ** p < 0.01; *** p < 0.001; ns—not significant; nd—not determined. Data shown as mean ± SEM.
Article Snippet: Gα i1 -RLuc8 (Addgene, #140973), Gα i2 -RLuc8 (Addgene, #140974), Gα i3 -RLuc8 (Addgene, #140975), Gα oA -RLuc8 (Addgene, #140976), Gα oB -RLuc8 (Addgene, #140977), Gβ 1 (Addgene, #140987), Gβ 2 (Addgene, #166772), Gβ 3 (Addgene, #140988), Gγ 3 -GFP2 (Addgene, #166775), Gγ 7 -GFP2 (Addgene, #166778),
Techniques: Activation Assay, Concentration Assay, Control, Activity Assay, Comparison
Journal: STAR Protocols
Article Title: Protocol for quantitative evaluation of misfolded protein degradation using Agrobacterium -mediated expression system in Nicotiana benthamiana
doi: 10.1016/j.xpro.2024.103034
Figure Lengend Snippet:
Article Snippet:
Techniques: Virus, Recombinant, Protease Inhibitor, Cloning, DNA Extraction, Plasmid Preparation, Marker, Western Blot, Amplification, Software, Membrane, Electrophoresis, Fertilizer