gfp-tank Search Results


human tank gene orf cdna clone expression plasmid, c-gfpspark tag  (Sino Biological)
N/A
Full length Clone DNA of Human TRAF family member-associated NFKB activator with C terminal GFPSpark tag.
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gfp tbk1 s172e  (Proteintech)
96
Proteintech gfp tbk1 s172e
a , b Western blots of WT or TBC1D9 -KO cells during GAS infection. a HeLa WT or TBC1D9 -KO cells were infected with GAS for the indicated times and analyzed by western blot. b Quantification of the p-TBK1/TBK1 ratio. c , d HeLa WT, TBC1D9 -KO, TBC1D9 -overexpressing TBC1D9 -KO, and TBC1D9 R566K -overexpressing TBC1D9 -KO cells were infected with GAS for 4 h, fixed, and immunostained for ubiquitin (magenta) and p-TBK1 (green). Cellular and bacterial DNA were stained with DAPI (cyan). c Representative confocal images and d quantification of p-TBK1-positive ubiquitin-coated GAS. e Coimmunoprecipitation of GFP-TBC1D9 and FLAG-TBK1. f Coimmunoprecipitation of endogenous TBC1D9 and TBK1. g Coimmunoprecipitation of GFP-TBK1 WT, S172A, K38A, and FLAG-TBC1D9. h Coimmunoprecipitation of GFP-TBK1 and FLAG-TBK1 in GAS-infected HeLa WT or TBC1D9 -KO cells. i Coimmunoprecipitation of <t>GFP-TBK1-S172E</t> and FLAG-TBK1-S172E in HeLa WT or TBC1D9 -KO cells. Scale bars, 10 μm. Data in ( b ) and ( d ) ( n > 200 cells per condition) represent the mean ± SEM of three independent experiments. P values calculated by two-tailed Student’s t test.
Gfp Tbk1 S172e, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfp-tank/TBK1+Antibody/pmc07005872-276-18-27
Average 96 stars, based on 1 article reviews
gfp tbk1 s172e - by Bioz Stars, 2026-09
96/100 stars
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human tank gene lentiviral orf cdna expression plasmid, c-gfpspark tag  (Sino Biological)
N/A
Full length Clone DNA of Homo sapiens TRAF family member-associated NFKB activator.
   Buy from Supplier
human tank gene orf cdna clone expression plasmid, n-gfpspark tag  (Sino Biological)
N/A
Full length Clone DNA of Human TRAF family member-associated NFKB activator with N terminal GFPSpark tag.
   Buy from Supplier

Image Search Results


a , b Western blots of WT or TBC1D9 -KO cells during GAS infection. a HeLa WT or TBC1D9 -KO cells were infected with GAS for the indicated times and analyzed by western blot. b Quantification of the p-TBK1/TBK1 ratio. c , d HeLa WT, TBC1D9 -KO, TBC1D9 -overexpressing TBC1D9 -KO, and TBC1D9 R566K -overexpressing TBC1D9 -KO cells were infected with GAS for 4 h, fixed, and immunostained for ubiquitin (magenta) and p-TBK1 (green). Cellular and bacterial DNA were stained with DAPI (cyan). c Representative confocal images and d quantification of p-TBK1-positive ubiquitin-coated GAS. e Coimmunoprecipitation of GFP-TBC1D9 and FLAG-TBK1. f Coimmunoprecipitation of endogenous TBC1D9 and TBK1. g Coimmunoprecipitation of GFP-TBK1 WT, S172A, K38A, and FLAG-TBC1D9. h Coimmunoprecipitation of GFP-TBK1 and FLAG-TBK1 in GAS-infected HeLa WT or TBC1D9 -KO cells. i Coimmunoprecipitation of GFP-TBK1-S172E and FLAG-TBK1-S172E in HeLa WT or TBC1D9 -KO cells. Scale bars, 10 μm. Data in ( b ) and ( d ) ( n > 200 cells per condition) represent the mean ± SEM of three independent experiments. P values calculated by two-tailed Student’s t test.

Journal: Nature Communications

Article Title: TBC1D9 regulates TBK1 activation through Ca 2+ signaling in selective autophagy

doi: 10.1038/s41467-020-14533-4

Figure Lengend Snippet: a , b Western blots of WT or TBC1D9 -KO cells during GAS infection. a HeLa WT or TBC1D9 -KO cells were infected with GAS for the indicated times and analyzed by western blot. b Quantification of the p-TBK1/TBK1 ratio. c , d HeLa WT, TBC1D9 -KO, TBC1D9 -overexpressing TBC1D9 -KO, and TBC1D9 R566K -overexpressing TBC1D9 -KO cells were infected with GAS for 4 h, fixed, and immunostained for ubiquitin (magenta) and p-TBK1 (green). Cellular and bacterial DNA were stained with DAPI (cyan). c Representative confocal images and d quantification of p-TBK1-positive ubiquitin-coated GAS. e Coimmunoprecipitation of GFP-TBC1D9 and FLAG-TBK1. f Coimmunoprecipitation of endogenous TBC1D9 and TBK1. g Coimmunoprecipitation of GFP-TBK1 WT, S172A, K38A, and FLAG-TBC1D9. h Coimmunoprecipitation of GFP-TBK1 and FLAG-TBK1 in GAS-infected HeLa WT or TBC1D9 -KO cells. i Coimmunoprecipitation of GFP-TBK1-S172E and FLAG-TBK1-S172E in HeLa WT or TBC1D9 -KO cells. Scale bars, 10 μm. Data in ( b ) and ( d ) ( n > 200 cells per condition) represent the mean ± SEM of three independent experiments. P values calculated by two-tailed Student’s t test.

Article Snippet: For the binding experiment shown in Fig. , lysate of HeLa WT or TBC1D9 -KO cells transfected with GFP-TBK1 S172E and FLAG-TBK1 S172E were immunoprecipitated with GFP-Trap (ChromoTek).

Techniques: Western Blot, Infection, Ubiquitin Proteomics, Staining, Two Tailed Test