Journal: Nature Communications

Article Title: Neuropeptide SP protects against colitis and linked anxiety-like behavior through the putative roles of gut microbiota and metabolite inositol

doi: 10.1038/s41467-025-67904-0

Figure Lengend Snippet: a Diagram illustrating the mouse model of colitis employed in this study, and inositol treatments are indicated (Some schematic elements were created by Figdraw.com). b Daily body weight changes. n = 8 mice/group. c Diseases activity index. n = 6 mice/group. d Macroscopic pictures of colons. e H&E-stained colon sections. Scale bar = 100 μm. n = 5 independent experiments. f Immunofluorescence staining for E-cadherin (red)/DAPI (blue) in colon tissues. Scale bar = 100 μm. n = 4 independent experiments. g The mRNA level of inflammatory cytokines ( IL-1β , IL-6 and TNF-α ) in the colon. n = 4 mice/group. h , i Representative movement tracks in the open field test and related bar graphs (Distance traveled, distance of center region and speed). n = 6 mice/group. j , k Track plot of the elevated plus maze, and statistical analysis including percentage of time spent in the open arms and percentage of times entering the open arms. n = 6 mice/group. l Representative H&E-stained hippocampus sections of three groups. Scale bar = 100 μm. n = 6 independent experiments. m Photomicrographs of Nissl staining in the hippocampus. Scale bar = 100 μm. n = 6 independent experiments. n The mRNA expressions of downstream cytokines ( IL-6 , IL-1β , TNF-α and IL-10 ) in the hippocampus tissue. n = 5 mice/group. o Representative immunohistochemistry images of Iba-1 in hippocampus, Scale bar = 100 μm. n = 3 independent experiments. p The concentration of GABA in hippocampus tissue. n = 6 mice/group. q The mRNA expression of GABA A Rα1 in hippocampus. n = 5 mice/group. r The protein level of GABA B R detected by western blot. n = 3 independent experiments. s Double immunofluorescence staining for GFAP (red)/GAT1 (green) in the DG and CA1 regions of hippocampus tissue. Scale bar = 50 μm. n = 3 independent experiments. Data were presented as means ± SD. For body weight change, two-way repeated-measures ANOVA was performed and the rest of the statistics was analyzed using one-way ANOVA followed by Tukey’s multiple comparisons test. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001. Source data are provided as a file.

Article Snippet: Briefly, after deparaffinization, rehydration, and antigen retrieval, slides were blocked with donkey serum for 30 min at room temperature following incubation with primary antibodies against GFAP (1:1000, A8335, 1:200, NB100-53809, USA), Iba-1 (DF6442, 1:200, Affinity, China), E-cadherin (PB9561, 1:200, Boster, China), Mucin 2 (bsm-60016R, 1:100, Bioss, China), GABA A Rα1 (1:200, 12708-1-AP, Proteintech, China), calcium/calmodulin-dependent protein kinase II (CaMKII) (1:50, ab76703, Abcam, UK) and p-IKBα (1:100, WLH3930, Wanleibio, China) at 4 C overnight.

Techniques: Activity Assay, Staining, Immunofluorescence, Immunohistochemistry, Concentration Assay, Expressing, Western Blot, Double Immunofluorescence Staining

Journal: Journal of biochemical and molecular toxicology

Article Title: Therapeutic effect of thymoquinone on brain damage caused by nonylphenol exposure in rats.

doi: 10.1002/jbt.23471

Figure Lengend Snippet: FIGURE 1 Graphical abstract. Cas‐3, caspase‐3; ELISA, enzyme‐linked immunosorbent assay; GFAP, glial fibrillary acidic protein; H&E, hematoxylin‐eosin; NeuN, neuronal nuclei; NGF, nerve growth factor; NOR, new object recognition; NP, nonylphenol; TQ, thymoquinone.

Article Snippet: Cas‐3 (Cat. No: E‐AB‐13815; Elabscience), NGF (Cat. No: E‐AB‐32239; Elabscience), GFAP (Cat. No: NB120‐16997; Novus Biologicals), and NeuN (Cat. No: FNab05669; Finetest) antibodies were prepared and instilled into the tissues.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of biochemical and molecular toxicology

Article Title: Therapeutic effect of thymoquinone on brain damage caused by nonylphenol exposure in rats.

doi: 10.1002/jbt.23471

Figure Lengend Snippet: FIGURE 4 Immunohistochemical staining of NeuN, GFAP, Cas‐3, and NGF in experimental groups. Black arrow indicates immunohistochemically stained structures. The yellow arrow indicate structures that are not or understained immunohistochemically. (A) Control, (B) nonylphenol, (C) nonylphenol + thymoquinone, (D) thymoquinone. Immunohistochemical staining, Olympus microscope, ×400. Cas‐ 3, caspase‐3; GFAP, glial fibrillary acidic protein; NeuN, neuronal nuclei; NGF, nerve growth factor.

Article Snippet: Cas‐3 (Cat. No: E‐AB‐13815; Elabscience), NGF (Cat. No: E‐AB‐32239; Elabscience), GFAP (Cat. No: NB120‐16997; Novus Biologicals), and NeuN (Cat. No: FNab05669; Finetest) antibodies were prepared and instilled into the tissues.

Techniques: Immunohistochemical staining, Staining, Control, Microscopy

Journal: Journal of biochemical and molecular toxicology

Article Title: Therapeutic effect of thymoquinone on brain damage caused by nonylphenol exposure in rats.

doi: 10.1002/jbt.23471

Figure Lengend Snippet: FIGURE 5 Statistical analysis of NeuN, GFAP, Cas‐3, and NGF immunoreactivity of the experimental groups. Cas‐3, caspase‐3; GFAP, glial fibrillary acidic protein; NeuN, neuronal nuclei; NGF, nerve growth factor; NP, nonylphenol; TQ, thymoquinone.

Article Snippet: Cas‐3 (Cat. No: E‐AB‐13815; Elabscience), NGF (Cat. No: E‐AB‐32239; Elabscience), GFAP (Cat. No: NB120‐16997; Novus Biologicals), and NeuN (Cat. No: FNab05669; Finetest) antibodies were prepared and instilled into the tissues.

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Integrating network pharmacology and transcriptomics to reveal the therapeutic effect of Long Mu Ning Xin Decoction on attention-deficit/hyperactivity disorder by regulating cAMP and PI3K/AKT pathways

doi: 10.3389/fphar.2026.1744709

Figure Lengend Snippet: Effects of LMNXD on the relative expression of GFAP and DRD1 positive Area% in the hippocampus and PFC of rats and BDNF protein in the striatum. (A) Immunofluorescence staining of GFAP (Scale: 100um). (B) Immunohistochemical staining of DRD1 (Scale: 200um). (C) Western blotting analysis image of BDNF in the striatum. (D) Relative GFAP positive area%. (E) Relative DRD1 positive area%. (F) Relative expression of BDNF proteins. Data are expressed as mean ± standard deviation, n = 3 in immunohistochemistry and immunofluorescence, n = 6 in Western blot. Compared with the WKY group, # p < 0.05, ## p < 0.01. Compared with the SHR group, * p < 0.05, ** p < 0.01, **** p < 0.0001.

Article Snippet: Primary antibodies against glial fibrillary acidic protein (GFAP, 60190-1-Ig), dopamine deceptor D1 (DRD1, 17934-1-Ap), and BDNF (66292-1-Ig) were obtained from Proteintech (Wuhan, China).

Techniques: Expressing, Immunofluorescence, Staining, Immunohistochemical staining, Western Blot, Standard Deviation, Immunohistochemistry