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Image Search Results
Journal: International Journal of Biological Sciences
Article Title: Chimerization of human ESC-derived extraembryonic cells with the mouse blastocyst
doi: 10.7150/ijbs.99519
Figure Lengend Snippet: Verification of the pluripotency of MSX2 -/- hESCs and enhanced ability to differentiate to TBs. (A) Genotyping results of two MSX2 -/- hES cell clones. (B) Immunostaining for pluripotency makers SSEA4, NANOG, OCT4, and SOX2 in MSX2 -/- hESCs. Scale bar, 100 μm. (C) Bright-field imaging and immunostaining for pluripotency marker SOX2 and mesodermal marker T in WT and MSX2 -/- hESCs following mesodermal differentiation for 2 days. Scale bar, 100 μm. (D) Western blotting for MSX2, SOX2, and T in WT and MSX2 -/- hESCs following mesodermal differentiation. GAPDH was tested as a loading control. (E) Bright-field photography of WT and MSX2 -/- hESCs before and after BAP treatment for 6 days. Scale bar, 400 μm. (F) QPCR detection of pluripotency markers in WT and MSX2 -/- hESCs after BAP treatment for 6 days. *** and $$$, P < 0.001 compared with WT hESCs; ###, P < 0.001 compared with MSX2 -/- hESCs. N = 3. (G) Immunostaining for TB markers KRT7, TP63, GATA3, and TFAP2C on MSX2 -/- hESCs treated with BAP medium for 6 days. Scale bar, 100 μm. (H) QPCR detection of TB markers on WT and MSX2 -/- hESCs before and after BAP treatment for 6 days. *** and $$$, P < 0.001 compared with WT hESCs; ###, P < 0.001 compared with MSX2 -/- hESCs. N = 3.
Article Snippet: Genomic DNA was extracted from each picked clone for
Techniques: Clone Assay, Immunostaining, Imaging, Marker, Western Blot, Control