Journal: Scientific Reports

Article Title: An inter-laboratory study to investigate the impact of the bioinformatics component on microbiome analysis using mock communities

doi: 10.1038/s41598-021-89881-2

Figure Lengend Snippet: The precision of MCM2α reported as percentage coefficient of variation (%CV) from triplicate technical repeats of each of the family of organisms reported by the 13 different laboratories for the 16S rRNA sequencing approach of MCM2α V12 (A), MCM2α V4–6 (B), MCM2β V1–2 (C), MCM2β V4–6 (D), and of the shotgun metagenomic sequencing approach for MCM2α (E) and MCM2β (F).

Article Snippet: Shotgun metagenomic sequencing was performed by LGC Genomics GmbH (Berlin, Germany).

Techniques: Sequencing

Journal: Metabolites

Article Title: What Has Longitudinal ‘Omics’ Studies Taught Us about Irritable Bowel Syndrome? A Systematic Review

doi: 10.3390/metabo13040484

Figure Lengend Snippet: The characteristics and key findings of the studies reviewed (arranged alphabetically by the first author’s last name).

Article Snippet: Ankersen, 2021 [ ] , Denmark , 34 IBS patients (either IBS-D or IBS-M) randomised to receive either web-based low FODMAP diet intervention or probiotic; Rome III criteria for diagnosis , Stool sample , CeGaT GmbH culture-independent, whole-genome, shotgun metagenomic, next-generation sequencing of genomic DNA , Four time points, during the course of 1 year , - web-based low FODMAP diet intervention and probiotic intervention were both efficacious in managing IBS symptoms - in both cases, treatment response could not be predicted or explained by the composition of host gut microbiota.

Techniques: Biomarker Discovery, Microarray, Real-time Polymerase Chain Reaction, Functional Assay, Expressing, Next-Generation Sequencing, Bacteria, Gene Expression, Genome Wide, Probiotics, Isolation, Amplification, Sequencing, Starch, Fluorescence, FACS, Concentration Assay, Control, Clinical Proteomics, RNA Sequencing, Modification