Journal: Microbiology Spectrum

Article Title: Bone allograft impregnated with tobramycin and vancomycin delivers antibiotics in high concentrations for prophylaxis against bacteria commonly associated with prosthetic joint infections

doi: 10.1128/spectrum.00414-24

Figure Lengend Snippet: Distribution of the MICs of gentamicin and tobramycin, for 139 isolates of S. epidermidis obtained from patients with prosthetic joint infections in Sweden.

Article Snippet: In this study, the TobraVanc solution was prepared by dissolving 1 g of vancomycin (Orion Pharma AB, Danderyd, Sweden) in 6 mL (480 mg) of tobramycin (generic version, Nebcina; Meda AB, Solna, Sweden) and 2 mL of saline.

Techniques:

Journal: Microbiology Spectrum

Article Title: Bone allograft impregnated with tobramycin and vancomycin delivers antibiotics in high concentrations for prophylaxis against bacteria commonly associated with prosthetic joint infections

doi: 10.1128/spectrum.00414-24

Figure Lengend Snippet: Standard curves relating the log concentrations of tobramycin and vancomycin to the inhibition zone diameter (mm). Each point on the dashed lines represents the mean of a duplicate observation. Linear regression lines are presented corresponding to the equation presented in the text.

Article Snippet: In this study, the TobraVanc solution was prepared by dissolving 1 g of vancomycin (Orion Pharma AB, Danderyd, Sweden) in 6 mL (480 mg) of tobramycin (generic version, Nebcina; Meda AB, Solna, Sweden) and 2 mL of saline.

Techniques: Inhibition

Journal: Microbiology Spectrum

Article Title: Bone allograft impregnated with tobramycin and vancomycin delivers antibiotics in high concentrations for prophylaxis against bacteria commonly associated with prosthetic joint infections

doi: 10.1128/spectrum.00414-24

Figure Lengend Snippet: Antibiotic concentrations assessed by using inhibition zone diameter for tobramycin and vancomycin used singly, as compared to the combination of both antibiotics (combined) for a triplicate series of concentrations ranging up to 60,000 mg/L for tobramycin and 125,000 mg/L for vancomycin. The agar plates were inoculated with (a) Escherichia coli ATCC 25922 (intrinsically resistant to vancomycin and susceptible to tobramycin) and (b) Enterococcus faecium AB202561 (resistant to tobramycin and susceptible to vancomycin).

Article Snippet: In this study, the TobraVanc solution was prepared by dissolving 1 g of vancomycin (Orion Pharma AB, Danderyd, Sweden) in 6 mL (480 mg) of tobramycin (generic version, Nebcina; Meda AB, Solna, Sweden) and 2 mL of saline.

Techniques: Inhibition

Journal: Microbiology Spectrum

Article Title: Bone allograft impregnated with tobramycin and vancomycin delivers antibiotics in high concentrations for prophylaxis against bacteria commonly associated with prosthetic joint infections

doi: 10.1128/spectrum.00414-24

Figure Lengend Snippet: Differences in antibiotic concentrations for (a) tobramycin and (b) vancomycin between bone graft chips collected from the femoral heads of six different donors (donors A to F), with the total weights of the prepared bone graft chips for each donor on the X -axis. Data are presented from samples taken at two time-points during acetabular revision surgery: (i) prior to application in the patient (pre-application; light-gray bars) and (ii) after application in the acetabular bone bed (post-application; dark-gray bars).

Article Snippet: In this study, the TobraVanc solution was prepared by dissolving 1 g of vancomycin (Orion Pharma AB, Danderyd, Sweden) in 6 mL (480 mg) of tobramycin (generic version, Nebcina; Meda AB, Solna, Sweden) and 2 mL of saline.

Techniques: