Journal: Biosensors

Article Title: Beyond Traditional Lateral Flow Assays: Enhancing Performance Through Multianalytical Strategies

doi: 10.3390/bios15020068

Figure Lengend Snippet: An overview of the reports regarding the analytical performance of multiplex LFAs.

Article Snippet: In another approach, again for meat adulteration detection, the pork ( Porcine ) mitochondrial ND2 gene and the chicken ( Gallus gallus ) cytochrome B gene were amplified by a dual RPA.

Techniques: Multiplex Assay, Virus, Bacteria, Fluorescence, Clinical Proteomics, Hybridization

Journal: Frontiers in Immunology

Article Title: Impact of bronchoalveolar lavage from influenza A virus diseased pigs on neutrophil functions and growth of co-infecting pathogenic bacteria

doi: 10.3389/fimmu.2024.1325269

Figure Lengend Snippet: Different markers in the BALF of respiratory diseased pigs tested negative (IAV-negative) and positive (IAV-positive) for IAV (A–D) and the correlation to the IAV Ct value detected in BALF and/or lung tissue (E–H) . The amounts of nicotinamide adenine dinucleotide (NAD) (A) , sialic acid (B) , IFN-α (C) , and IFN-γ (D) were analyzed in the BALF of 21 IAV-positive, 24 IAV-negative, and five (four in NAD) healthy control animals. For each marker, a box and whisker plot with the minimum and maximum values and individual points is presented. A Mann–Whitney test was calculated (* p < 0.05). The correlation of the analyzed markers and the Ct value of IAV was performed with Spearman r resulting in no significant correlation, except for a significant negative correlation between IAV Ct value and IFN-α (G) . A simple linear regression is depicted in the graph and the values for r , p , and n are given (E–H) . The dotted line (C, D, G, H) represents the detection limit of the ELISA for IFN-α (36 pg/ml) and IFN-γ (2 pg/ml), as all the samples where no signal was detected were set to the detection limit.

Article Snippet: The following assays were conducted: the General Nicotinamide Adenine Dinucleotide (NAD) ELISA Kit (Ref: MBS2700640-96, MyBioSource, San Diego, California, UnitedStates), to detect the amount of NAD; the Citrullinated Histone H3 (Clone 11D3) ELISA Kit (Ref: 501620, Lot: 0637729, Cayman Chemical Company, Ann Arbor, Michigan, United States), to detect the amount of citrullinated histone H3 (H3Cit); the Cell Death Detection ELISA PLUS Kit (Ref: 11774425001, Sigma), to detect the amount of nucleosomes; the Porcine IFN gamma (γ) ELISA Kit (Ref: KSC4021, Thermo Fisher Scientific Inc., Waltham, Massachusetts, United States), to detect IFN-γ; and the Porcine IFN alpha (α) ELISA Kit (Ref: ES7RB, Thermo Fisher Scientific Inc.), to detect IFN-α.

Techniques: Control, Marker, Whisker Assay, MANN-WHITNEY, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: Impact of bronchoalveolar lavage from influenza A virus diseased pigs on neutrophil functions and growth of co-infecting pathogenic bacteria

doi: 10.3389/fimmu.2024.1325269

Figure Lengend Snippet: Correlation analysis of NET markers and other markers detected in the BALF with the growth progression of A.pp in the BALF. The correlation analysis was performed with Spearman r for IAV-negative BALF (A, C, E, G, I, K, M, O, Q) and for IAV-positive BALF (B, D, F, H, J, L, N, P, R) and detected several significant positive correlations. The growth progression of A.pp correlates positively with cell-free DNA (A, B) , sialic acid (M, N) , and IFN-γ (Q, R) in the case of IAV-negative and IAV-positive BALF. The growth progression of A.pp correlates with the nucleosome (D) only in the case of IAV-positive BALF. The values for r , p , and n are given in each graph, and each square or triangle represents one BALF sample. Several markers in the BALF correlate positively with the growth progression of A.pp , but this was not significant. The dotted line (O–R) represents the detection limit of the ELISA for IFN-α (36 pg/ml) and IFN-γ (2 pg/ml), as all the samples where no signal was detected were set to the detection limit.

Article Snippet: The following assays were conducted: the General Nicotinamide Adenine Dinucleotide (NAD) ELISA Kit (Ref: MBS2700640-96, MyBioSource, San Diego, California, UnitedStates), to detect the amount of NAD; the Citrullinated Histone H3 (Clone 11D3) ELISA Kit (Ref: 501620, Lot: 0637729, Cayman Chemical Company, Ann Arbor, Michigan, United States), to detect the amount of citrullinated histone H3 (H3Cit); the Cell Death Detection ELISA PLUS Kit (Ref: 11774425001, Sigma), to detect the amount of nucleosomes; the Porcine IFN gamma (γ) ELISA Kit (Ref: KSC4021, Thermo Fisher Scientific Inc., Waltham, Massachusetts, United States), to detect IFN-γ; and the Porcine IFN alpha (α) ELISA Kit (Ref: ES7RB, Thermo Fisher Scientific Inc.), to detect IFN-α.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Mucosal Immunology

Article Title: Antibody secreting cells are critically dependent on integrin α4β7/MAdCAM-1 for intestinal recruitment and control of the microbiota during chronic colitis

doi: 10.1038/s41385-021-00445-z

Figure Lengend Snippet: a Percentage of CD11c + DC and CD4 + CD25 + FOXP3 + regulatory T cells within the colonic LP of the indicated mouse strains, as determined by flow cytometry (noninflamed (N/I), inflamed (I), as determined by histology). Events gated on live, single cells. b Aldefluor conversion within the colonic LP DC fraction isolated from the indicated strains. c Relative mRNA expression of RALDH1, RALDH2, and RALDH3 and d of regulatory cytokines and subunits within colonic tissues of indicated mouse strains. Expression of the respective genes was normalized to GAPDH expression. Each data point represents an individual mouse. Data are presented as mean ± S.D from n > 6 mice in each dataset. Statistical significance determined using ANOVA.

Article Snippet: Gene expression was examined using the following Taqman probes (all Thermo Fisher Scientific): TGFβ (Tgif1, Mm01227699_m1), EBI3 (Mm00469294_m1), IL-12A (Mm00434169_m1), RALDH1 (Aldh1a1, Mm00657317_m1), RALDH2 (Aldh1a2, Mm00501306_m1), and RALDH3 (Aldh1a3, Mm00474049_m1), and normalized to GAPDH (Mm99999915_g1).

Techniques: Flow Cytometry, Isolation, Expressing