gene expression Search Results


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OriGene amcl 1 gfp plasmid
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OriGene human plenti c mgfp control
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OriGene lentiviral gfp vector
(A) Transfection efficiency images of shRNA-C expressing a <t>lentiviral</t> vector for the knock-down of the CD66c gene. (B) Cell viability of BCSCs treated with shRNA-C under oxidative stress conditions. Data are expressed as the mean ± SEM. ** P < 0.02 for in the absence or presence of H 2 O 2 . *** P < 0.01 for H 2 O 2 -only group versus H 2 O 2 treated with shRNA-C group. (C) FACS analysis of cell death induced by treatment with H 2 O 2 post-shRNA-C transfection in BCSCs. (D) Quantitative viable cell population of BCSCs treated with shRNA-C and H 2 O 2 by FACS analysis. Data are expressed as the mean ± SEM. * P < 0.05 or ** P < 0.02 for the comparison of no H 2 O 2 treated groups and H 2 O 2 treated groups or ** P < 0.02 for shRNA-SC versus shRNA-C groups in the presence of H 2 O 2 . *** P < 0.01 for the comparison of NC and shRNA-C groups in the presence of H 2 O 2 . (E) Quantitative death cell population induced by shRNA-C treatment with H 2 O 2 . Data are expressed as the mean ± SEM. * P < 0.05 or ** P < 0.02 for in the absence or presence of H 2 O 2 . ** P < 0.02 for the comparison of shRNA-SC with H 2 O 2 group and shRNA-C with H 2 O 2 group. *** P < 0.01 for the comparison of H 2 O 2 only group and shRNA-C with H 2 O 2 group.
Lentiviral Gfp Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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OriGene plenti c cpsf1 mgfp p2a puro
(A) Transfection efficiency images of shRNA-C expressing a <t>lentiviral</t> vector for the knock-down of the CD66c gene. (B) Cell viability of BCSCs treated with shRNA-C under oxidative stress conditions. Data are expressed as the mean ± SEM. ** P < 0.02 for in the absence or presence of H 2 O 2 . *** P < 0.01 for H 2 O 2 -only group versus H 2 O 2 treated with shRNA-C group. (C) FACS analysis of cell death induced by treatment with H 2 O 2 post-shRNA-C transfection in BCSCs. (D) Quantitative viable cell population of BCSCs treated with shRNA-C and H 2 O 2 by FACS analysis. Data are expressed as the mean ± SEM. * P < 0.05 or ** P < 0.02 for the comparison of no H 2 O 2 treated groups and H 2 O 2 treated groups or ** P < 0.02 for shRNA-SC versus shRNA-C groups in the presence of H 2 O 2 . *** P < 0.01 for the comparison of NC and shRNA-C groups in the presence of H 2 O 2 . (E) Quantitative death cell population induced by shRNA-C treatment with H 2 O 2 . Data are expressed as the mean ± SEM. * P < 0.05 or ** P < 0.02 for in the absence or presence of H 2 O 2 . ** P < 0.02 for the comparison of shRNA-SC with H 2 O 2 group and shRNA-C with H 2 O 2 group. *** P < 0.01 for the comparison of H 2 O 2 only group and shRNA-C with H 2 O 2 group.
Plenti C Cpsf1 Mgfp P2a Puro, supplied by OriGene, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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OriGene plenti c myc ddk vector
(A) Transfection efficiency images of shRNA-C expressing a <t>lentiviral</t> vector for the knock-down of the CD66c gene. (B) Cell viability of BCSCs treated with shRNA-C under oxidative stress conditions. Data are expressed as the mean ± SEM. ** P < 0.02 for in the absence or presence of H 2 O 2 . *** P < 0.01 for H 2 O 2 -only group versus H 2 O 2 treated with shRNA-C group. (C) FACS analysis of cell death induced by treatment with H 2 O 2 post-shRNA-C transfection in BCSCs. (D) Quantitative viable cell population of BCSCs treated with shRNA-C and H 2 O 2 by FACS analysis. Data are expressed as the mean ± SEM. * P < 0.05 or ** P < 0.02 for the comparison of no H 2 O 2 treated groups and H 2 O 2 treated groups or ** P < 0.02 for shRNA-SC versus shRNA-C groups in the presence of H 2 O 2 . *** P < 0.01 for the comparison of NC and shRNA-C groups in the presence of H 2 O 2 . (E) Quantitative death cell population induced by shRNA-C treatment with H 2 O 2 . Data are expressed as the mean ± SEM. * P < 0.05 or ** P < 0.02 for in the absence or presence of H 2 O 2 . ** P < 0.02 for the comparison of shRNA-SC with H 2 O 2 group and shRNA-C with H 2 O 2 group. *** P < 0.01 for the comparison of H 2 O 2 only group and shRNA-C with H 2 O 2 group.
Plenti C Myc Ddk Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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OriGene plasmid plenti c myc ddk ires puro
(A) Transfection efficiency images of shRNA-C expressing a <t>lentiviral</t> vector for the knock-down of the CD66c gene. (B) Cell viability of BCSCs treated with shRNA-C under oxidative stress conditions. Data are expressed as the mean ± SEM. ** P < 0.02 for in the absence or presence of H 2 O 2 . *** P < 0.01 for H 2 O 2 -only group versus H 2 O 2 treated with shRNA-C group. (C) FACS analysis of cell death induced by treatment with H 2 O 2 post-shRNA-C transfection in BCSCs. (D) Quantitative viable cell population of BCSCs treated with shRNA-C and H 2 O 2 by FACS analysis. Data are expressed as the mean ± SEM. * P < 0.05 or ** P < 0.02 for the comparison of no H 2 O 2 treated groups and H 2 O 2 treated groups or ** P < 0.02 for shRNA-SC versus shRNA-C groups in the presence of H 2 O 2 . *** P < 0.01 for the comparison of NC and shRNA-C groups in the presence of H 2 O 2 . (E) Quantitative death cell population induced by shRNA-C treatment with H 2 O 2 . Data are expressed as the mean ± SEM. * P < 0.05 or ** P < 0.02 for in the absence or presence of H 2 O 2 . ** P < 0.02 for the comparison of shRNA-SC with H 2 O 2 group and shRNA-C with H 2 O 2 group. *** P < 0.01 for the comparison of H 2 O 2 only group and shRNA-C with H 2 O 2 group.
Plasmid Plenti C Myc Ddk Ires Puro, supplied by OriGene, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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OriGene plenti ef1a c myc ddkires puro
(A) Transfection efficiency images of shRNA-C expressing a <t>lentiviral</t> vector for the knock-down of the CD66c gene. (B) Cell viability of BCSCs treated with shRNA-C under oxidative stress conditions. Data are expressed as the mean ± SEM. ** P < 0.02 for in the absence or presence of H 2 O 2 . *** P < 0.01 for H 2 O 2 -only group versus H 2 O 2 treated with shRNA-C group. (C) FACS analysis of cell death induced by treatment with H 2 O 2 post-shRNA-C transfection in BCSCs. (D) Quantitative viable cell population of BCSCs treated with shRNA-C and H 2 O 2 by FACS analysis. Data are expressed as the mean ± SEM. * P < 0.05 or ** P < 0.02 for the comparison of no H 2 O 2 treated groups and H 2 O 2 treated groups or ** P < 0.02 for shRNA-SC versus shRNA-C groups in the presence of H 2 O 2 . *** P < 0.01 for the comparison of NC and shRNA-C groups in the presence of H 2 O 2 . (E) Quantitative death cell population induced by shRNA-C treatment with H 2 O 2 . Data are expressed as the mean ± SEM. * P < 0.05 or ** P < 0.02 for in the absence or presence of H 2 O 2 . ** P < 0.02 for the comparison of shRNA-SC with H 2 O 2 group and shRNA-C with H 2 O 2 group. *** P < 0.01 for the comparison of H 2 O 2 only group and shRNA-C with H 2 O 2 group.
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93
OriGene ps100102
(A) Transfection efficiency images of shRNA-C expressing a <t>lentiviral</t> vector for the knock-down of the CD66c gene. (B) Cell viability of BCSCs treated with shRNA-C under oxidative stress conditions. Data are expressed as the mean ± SEM. ** P < 0.02 for in the absence or presence of H 2 O 2 . *** P < 0.01 for H 2 O 2 -only group versus H 2 O 2 treated with shRNA-C group. (C) FACS analysis of cell death induced by treatment with H 2 O 2 post-shRNA-C transfection in BCSCs. (D) Quantitative viable cell population of BCSCs treated with shRNA-C and H 2 O 2 by FACS analysis. Data are expressed as the mean ± SEM. * P < 0.05 or ** P < 0.02 for the comparison of no H 2 O 2 treated groups and H 2 O 2 treated groups or ** P < 0.02 for shRNA-SC versus shRNA-C groups in the presence of H 2 O 2 . *** P < 0.01 for the comparison of NC and shRNA-C groups in the presence of H 2 O 2 . (E) Quantitative death cell population induced by shRNA-C treatment with H 2 O 2 . Data are expressed as the mean ± SEM. * P < 0.05 or ** P < 0.02 for in the absence or presence of H 2 O 2 . ** P < 0.02 for the comparison of shRNA-SC with H 2 O 2 group and shRNA-C with H 2 O 2 group. *** P < 0.01 for the comparison of H 2 O 2 only group and shRNA-C with H 2 O 2 group.
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Image Search Results


(A) Transfection efficiency images of shRNA-C expressing a lentiviral vector for the knock-down of the CD66c gene. (B) Cell viability of BCSCs treated with shRNA-C under oxidative stress conditions. Data are expressed as the mean ± SEM. ** P < 0.02 for in the absence or presence of H 2 O 2 . *** P < 0.01 for H 2 O 2 -only group versus H 2 O 2 treated with shRNA-C group. (C) FACS analysis of cell death induced by treatment with H 2 O 2 post-shRNA-C transfection in BCSCs. (D) Quantitative viable cell population of BCSCs treated with shRNA-C and H 2 O 2 by FACS analysis. Data are expressed as the mean ± SEM. * P < 0.05 or ** P < 0.02 for the comparison of no H 2 O 2 treated groups and H 2 O 2 treated groups or ** P < 0.02 for shRNA-SC versus shRNA-C groups in the presence of H 2 O 2 . *** P < 0.01 for the comparison of NC and shRNA-C groups in the presence of H 2 O 2 . (E) Quantitative death cell population induced by shRNA-C treatment with H 2 O 2 . Data are expressed as the mean ± SEM. * P < 0.05 or ** P < 0.02 for in the absence or presence of H 2 O 2 . ** P < 0.02 for the comparison of shRNA-SC with H 2 O 2 group and shRNA-C with H 2 O 2 group. *** P < 0.01 for the comparison of H 2 O 2 only group and shRNA-C with H 2 O 2 group.

Journal: Molecules and Cells

Article Title: Biological Functions and Identification of Novel Biomarker Expressed on the Surface of Breast Cancer-Derived Cancer Stem Cells via Proteomic Analysis

doi: 10.14348/molcells.2020.2230

Figure Lengend Snippet: (A) Transfection efficiency images of shRNA-C expressing a lentiviral vector for the knock-down of the CD66c gene. (B) Cell viability of BCSCs treated with shRNA-C under oxidative stress conditions. Data are expressed as the mean ± SEM. ** P < 0.02 for in the absence or presence of H 2 O 2 . *** P < 0.01 for H 2 O 2 -only group versus H 2 O 2 treated with shRNA-C group. (C) FACS analysis of cell death induced by treatment with H 2 O 2 post-shRNA-C transfection in BCSCs. (D) Quantitative viable cell population of BCSCs treated with shRNA-C and H 2 O 2 by FACS analysis. Data are expressed as the mean ± SEM. * P < 0.05 or ** P < 0.02 for the comparison of no H 2 O 2 treated groups and H 2 O 2 treated groups or ** P < 0.02 for shRNA-SC versus shRNA-C groups in the presence of H 2 O 2 . *** P < 0.01 for the comparison of NC and shRNA-C groups in the presence of H 2 O 2 . (E) Quantitative death cell population induced by shRNA-C treatment with H 2 O 2 . Data are expressed as the mean ± SEM. * P < 0.05 or ** P < 0.02 for in the absence or presence of H 2 O 2 . ** P < 0.02 for the comparison of shRNA-SC with H 2 O 2 group and shRNA-C with H 2 O 2 group. *** P < 0.01 for the comparison of H 2 O 2 only group and shRNA-C with H 2 O 2 group.

Article Snippet: A total of 1 × 10 5 BCSCs sorted by the isolation kit were transfected with shRNA-CD66c in lentiviral GFP vector (Origene, USA) or shRNA-scrambled (SC) in lentiviral GFP vector (Origene) using TransIT-X2 (Mirus Bio, USA), following the manufacturer’s instructions.

Techniques: Transfection, shRNA, Expressing, Plasmid Preparation, Knockdown, Comparison