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Image Search Results
Journal: Molecules and Cells
Article Title: Biological Functions and Identification of Novel Biomarker Expressed on the Surface of Breast Cancer-Derived Cancer Stem Cells via Proteomic Analysis
doi: 10.14348/molcells.2020.2230
Figure Lengend Snippet: (A) Transfection efficiency images of shRNA-C expressing a lentiviral vector for the knock-down of the CD66c gene. (B) Cell viability of BCSCs treated with shRNA-C under oxidative stress conditions. Data are expressed as the mean ± SEM. ** P < 0.02 for in the absence or presence of H 2 O 2 . *** P < 0.01 for H 2 O 2 -only group versus H 2 O 2 treated with shRNA-C group. (C) FACS analysis of cell death induced by treatment with H 2 O 2 post-shRNA-C transfection in BCSCs. (D) Quantitative viable cell population of BCSCs treated with shRNA-C and H 2 O 2 by FACS analysis. Data are expressed as the mean ± SEM. * P < 0.05 or ** P < 0.02 for the comparison of no H 2 O 2 treated groups and H 2 O 2 treated groups or ** P < 0.02 for shRNA-SC versus shRNA-C groups in the presence of H 2 O 2 . *** P < 0.01 for the comparison of NC and shRNA-C groups in the presence of H 2 O 2 . (E) Quantitative death cell population induced by shRNA-C treatment with H 2 O 2 . Data are expressed as the mean ± SEM. * P < 0.05 or ** P < 0.02 for in the absence or presence of H 2 O 2 . ** P < 0.02 for the comparison of shRNA-SC with H 2 O 2 group and shRNA-C with H 2 O 2 group. *** P < 0.01 for the comparison of H 2 O 2 only group and shRNA-C with H 2 O 2 group.
Article Snippet: A total of 1 × 10 5 BCSCs sorted by the isolation kit were transfected with shRNA-CD66c in
Techniques: Transfection, shRNA, Expressing, Plasmid Preparation, Knockdown, Comparison