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Image Search Results
Journal: Clinical & experimental metastasis
Article Title: Automated Quantitative Image Analysis for ex vivo Metastasis Assays Reveals Differing Lung Composition Requirements for Metastasis Suppression by KISS1
doi: 10.1007/s10585-018-9882-1
Figure Lengend Snippet: PuMA image acquisition and automated image analysis workflow. (a) Mouse lung sections (0.5–1mm thick) containing disseminated GFP-expressing melanoma cells were placed on media-saturated Gelfoam® sponges cultured ex vivo for up to three weeks. Using an inverted microscope, an average of 30 non-redundant photomicrographs were recorded per experimental group every seven days. Scale bar for cell culture dish is 1 cm. Scale bar for fluorescent image is 50 μm. (b) Cross-section views of lung sections containing GFP-expressing melanoma cells within lung section atop Gelfoam® with respect to the focal plane of the image. (c) Schematic of the automated workflow used for image analysis. Output from a logistic regression model selects analyzable images for subsequent automated analysis. To standardize measurement of melanoma growth, only in-focus fluorescent cell clusters/regions of interest (ROI) were quantified. In-focus ROI were selected by applying a Gaussian gradient and selecting ROI with a clarity value above the in-focus threshold for quantification. Scale bars are 50μm.
Article Snippet: Each
Techniques: Expressing, Cell Culture, Ex Vivo, Inverted Microscopy
Journal: Journal of Orthopaedic Surgery and Research
Article Title: Differential expression of type X collagen in a mechanically active 3-D chondrocyte culture system: a quantitative study
doi: 10.1186/1749-799X-1-15
Figure Lengend Snippet: Photograph and line drawing of the Gelfoam sponge loaded in a square petri dish with a 6 by 7 grid of dots marked on surface. The stationary clamp edge is on left, and mobile plastic clip-metal bar assembly is on right.
Article Snippet:
Techniques: