gel Search Results


gel filtration calibration kit  (Cytiva Europe)
94
Cytiva Europe gel filtration calibration kit
(A) SDS-PAGE analyses of the crude cell extract of P. pastoris cells expressing AxyC and purified AxyC. Black triangle represents protein band of AxyC. M: molecular marker. (B) Gel <t>filtration</t> chromatography elution profile of AxyC. <t>Calibration</t> curve used to estimate molecular weight of AxyC is indicated by a dashed line. Dots indicate molecular weight of marker proteins: thyroglobulin (669×10 3 ), ferritin (440×10 3 ), aldolase (158×10 3 ), conalbumin (75×10 3 ), ovalbumin (43×10 3 ), carbonic anhydrase (29×10 3 ), and ribonuclease A (RNase A; 13.7×10 3 ).
Gel Filtration Calibration Kit, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gel microcilia array  (Microelectrodes Inc)
86
Microelectrodes Inc gel microcilia array
(A) SDS-PAGE analyses of the crude cell extract of P. pastoris cells expressing AxyC and purified AxyC. Black triangle represents protein band of AxyC. M: molecular marker. (B) Gel <t>filtration</t> chromatography elution profile of AxyC. <t>Calibration</t> curve used to estimate molecular weight of AxyC is indicated by a dashed line. Dots indicate molecular weight of marker proteins: thyroglobulin (669×10 3 ), ferritin (440×10 3 ), aldolase (158×10 3 ), conalbumin (75×10 3 ), ovalbumin (43×10 3 ), carbonic anhydrase (29×10 3 ), and ribonuclease A (RNase A; 13.7×10 3 ).
Gel Microcilia Array, supplied by Microelectrodes Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gel permeation chromatography  (Tosoh Corporation)
86
Tosoh Corporation gel permeation chromatography
(A) SDS-PAGE analyses of the crude cell extract of P. pastoris cells expressing AxyC and purified AxyC. Black triangle represents protein band of AxyC. M: molecular marker. (B) Gel <t>filtration</t> chromatography elution profile of AxyC. <t>Calibration</t> curve used to estimate molecular weight of AxyC is indicated by a dashed line. Dots indicate molecular weight of marker proteins: thyroglobulin (669×10 3 ), ferritin (440×10 3 ), aldolase (158×10 3 ), conalbumin (75×10 3 ), ovalbumin (43×10 3 ), carbonic anhydrase (29×10 3 ), and ribonuclease A (RNase A; 13.7×10 3 ).
Gel Permeation Chromatography, supplied by Tosoh Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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silika gel  (Merck & Co)
86
Merck & Co silika gel
(A) SDS-PAGE analyses of the crude cell extract of P. pastoris cells expressing AxyC and purified AxyC. Black triangle represents protein band of AxyC. M: molecular marker. (B) Gel <t>filtration</t> chromatography elution profile of AxyC. <t>Calibration</t> curve used to estimate molecular weight of AxyC is indicated by a dashed line. Dots indicate molecular weight of marker proteins: thyroglobulin (669×10 3 ), ferritin (440×10 3 ), aldolase (158×10 3 ), conalbumin (75×10 3 ), ovalbumin (43×10 3 ), carbonic anhydrase (29×10 3 ), and ribonuclease A (RNase A; 13.7×10 3 ).
Silika Gel, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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silica gel 60 f254 sentana  (Merck & Co)
86
Merck & Co silica gel 60 f254 sentana
(A) SDS-PAGE analyses of the crude cell extract of P. pastoris cells expressing AxyC and purified AxyC. Black triangle represents protein band of AxyC. M: molecular marker. (B) Gel <t>filtration</t> chromatography elution profile of AxyC. <t>Calibration</t> curve used to estimate molecular weight of AxyC is indicated by a dashed line. Dots indicate molecular weight of marker proteins: thyroglobulin (669×10 3 ), ferritin (440×10 3 ), aldolase (158×10 3 ), conalbumin (75×10 3 ), ovalbumin (43×10 3 ), carbonic anhydrase (29×10 3 ), and ribonuclease A (RNase A; 13.7×10 3 ).
Silica Gel 60 F254 Sentana, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gel beads  (10X Genomics)
86
10X Genomics gel beads
(A) SDS-PAGE analyses of the crude cell extract of P. pastoris cells expressing AxyC and purified AxyC. Black triangle represents protein band of AxyC. M: molecular marker. (B) Gel <t>filtration</t> chromatography elution profile of AxyC. <t>Calibration</t> curve used to estimate molecular weight of AxyC is indicated by a dashed line. Dots indicate molecular weight of marker proteins: thyroglobulin (669×10 3 ), ferritin (440×10 3 ), aldolase (158×10 3 ), conalbumin (75×10 3 ), ovalbumin (43×10 3 ), carbonic anhydrase (29×10 3 ), and ribonuclease A (RNase A; 13.7×10 3 ).
Gel Beads, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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resolving gel buffer for page  (Bio-Rad)
93
Bio-Rad resolving gel buffer for page
a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing <t>gel</t> <t>electrophoresis</t> with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).
Resolving Gel Buffer For Page, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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t4 polynucleotide kinase  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology t4 polynucleotide kinase
a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing <t>gel</t> <t>electrophoresis</t> with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).
T4 Polynucleotide Kinase, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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sds polyacrylamide gel electrophoresis  (Rockland Immunochemicals)
93
Rockland Immunochemicals sds polyacrylamide gel electrophoresis
a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing <t>gel</t> <t>electrophoresis</t> with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).
Sds Polyacrylamide Gel Electrophoresis, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gel filtration standards  (Bio-Rad)
96
Bio-Rad gel filtration standards
a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing <t>gel</t> <t>electrophoresis</t> with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).
Gel Filtration Standards, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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oriole fluorescent gel stain  (Bio-Rad)
95
Bio-Rad oriole fluorescent gel stain
a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing <t>gel</t> <t>electrophoresis</t> with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).
Oriole Fluorescent Gel Stain, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gel trays  (Bio-Rad)
93
Bio-Rad gel trays
a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing <t>gel</t> <t>electrophoresis</t> with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).
Gel Trays, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) SDS-PAGE analyses of the crude cell extract of P. pastoris cells expressing AxyC and purified AxyC. Black triangle represents protein band of AxyC. M: molecular marker. (B) Gel filtration chromatography elution profile of AxyC. Calibration curve used to estimate molecular weight of AxyC is indicated by a dashed line. Dots indicate molecular weight of marker proteins: thyroglobulin (669×10 3 ), ferritin (440×10 3 ), aldolase (158×10 3 ), conalbumin (75×10 3 ), ovalbumin (43×10 3 ), carbonic anhydrase (29×10 3 ), and ribonuclease A (RNase A; 13.7×10 3 ).

Journal: Journal of Applied Glycoscience

Article Title: Identification and Characterization of Novel Intracellular α-Xylosidase in Aspergillus oryzae

doi: 10.5458/jag.jag.JAG-2023_0007

Figure Lengend Snippet: (A) SDS-PAGE analyses of the crude cell extract of P. pastoris cells expressing AxyC and purified AxyC. Black triangle represents protein band of AxyC. M: molecular marker. (B) Gel filtration chromatography elution profile of AxyC. Calibration curve used to estimate molecular weight of AxyC is indicated by a dashed line. Dots indicate molecular weight of marker proteins: thyroglobulin (669×10 3 ), ferritin (440×10 3 ), aldolase (158×10 3 ), conalbumin (75×10 3 ), ovalbumin (43×10 3 ), carbonic anhydrase (29×10 3 ), and ribonuclease A (RNase A; 13.7×10 3 ).

Article Snippet: A calibration curve used to estimate the molecular weight of AxyC was constructed using marker proteins (Gel Filtration Calibration Kit, HMW and LMW; Cytiva).

Techniques: SDS Page, Expressing, Purification, Marker, Filtration, Chromatography, Molecular Weight

a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing gel electrophoresis with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).

Journal: Nature

Article Title: SIGLEC12 mediates plasma membrane rupture during necroptotic cell death

doi: 10.1038/s41586-025-09741-1

Figure Lengend Snippet: a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing gel electrophoresis with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).

Article Snippet: The following reagents were used in this study: SM-164 (S7089), Nec-1s (S8641), GSK′872 (S8465), NSA (S8251), emricasan (S7775) and erastin (S7242) from SelleckChem; etoposide (E1383), α-ketoglutarate (349631), lipopolysaccharide (L4391), luminol (A8511), p -coumaric acid (C9008) and anti-FLAG M2 agarose beads (M8823) from Sigma-Aldrich; nigericin (11437) from the Cayman Chemical Company; Lipofectamine 3000 (L3000015), SuperSignal West Atto Ultimate Sensitivity Substrate (A38556) and Prolong Diamond Antifade Mountant with DAPI ( P36966 ) from Thermo Fisher Scientific; polyethylenimine (PEI; 24765-100) from Kyfora Bio; polybrene (TR-1003) from EMD Millipore; and 10X Tris/Glycine/SDS Electrophoresis Buffer (1610772), Tween 20 (1610781), Stacking Gel Buffer for PAGE (1610799), Resolving Gel Buffer for PAGE (1610798), Precision Plus Protein Dual Color Standards (1610394) and nitrocellulose membrane (1620115) from Bio-Rad.

Techniques: Nucleic Acid Electrophoresis, shRNA, Fluorescence, Confocal Microscopy, Electron Microscopy, Western Blot, Two Tailed Test