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Image Search Results
Journal: iScience
Article Title: Chemerin 15 enhances microglial phagocytosis to attenuate cerebral ischemia-reperfusion injury through the ChemR23/p38 MAPK pathway
doi: 10.1016/j.isci.2025.113396
Figure Lengend Snippet: Effect of C15/ChemR23 on HMGB1 levels and ROS production in vivo (A) Western blot was used to measure the protein expression of HMGB1 after I/R in mice. (B) Densitometric quantification of HMGB1/β-actin expression ( n = 4, independent samples). (C) Representative DHE staining images show the production of ROS (red) after I/R in mice. Scale bar: 100 μm. (D) The bar graph represents the mean fluorescent intensity (MFI) of ROS ( n = 6, independent samples). Data are presented as means ± SDs and were analyzed using one-way ANOVA with post hoc Tukey’s multiple comparison test. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
Article Snippet: The following were the primary antibodies used: anti-phospho-p38 MAPK (Thr180/Tyr182) rabbit monoclonal antibody (No. 4511T, Cell Signaling Technology, Danvers, MA, USA); anti-p38 MAPK rabbit monoclonal antibody (No. 8690T, Cell Signaling Technology); anti-HMGB1 rabbit monoclonal antibody (No. ab79823, Abcam); and
Techniques: In Vivo, Western Blot, Expressing, Staining, Comparison