Journal: Journal of Neuroscience

Article Title: CD200 Ligand Receptor Interaction Modulates Microglial Activation In Vivo and In Vitro: A Role for IL-4

doi: 10.1523/jneurosci.1781-07.2007

Figure Lengend Snippet: Figure1. CD200isexpressedonneuronsandisreducedinchronicneuroinflammatorycon- ditions.A,DoubleimmunofluorescenceforCD200(i)andIII-tubulin(ii)andamergedimage (iii) in cultured neurons. B, Double immunofluorescence for MHCII (i) and CD200R (ii) and a merged image (iii) in mixed cultured glia treated with A. C, CD200 protein expression de- creases with age as shown by Western blot (*p 0.05; n 13). D, Fluorescent images of CD200 in the dentate gyrus of young (i) and aged (ii) animals. E, Age-related increase in the expression of MHCII mRNA (***p 0.001; n 13). F, Images of MHCII staining in the hip- pocampalCA1regionofyoung(i)andaged(ii)animals.Scalebars:A,B,20M;D,10M;F,50 M. Error bars indicate SEM.

Article Snippet: Cells and sections were treated overnight at 4°C as follows: mouse monoclonal CD200 antibody (1:200; Abcam, Cambridge, UK), mouse monoclonal MHCII antibody (1:100; Serotec), goat polyclonal CD200R antibody (1:100; Santa Cruz Biotechnology), and mouse monoclonal III-tubulin antibody (1:200; Chemicon).

Techniques: Cell Culture, Immunofluorescence, Expressing, Western Blot, Staining

Journal: Journal of Neuroscience

Article Title: CD200 Ligand Receptor Interaction Modulates Microglial Activation In Vivo and In Vitro: A Role for IL-4

doi: 10.1523/jneurosci.1781-07.2007

Figure Lengend Snippet: Figure 2. CD200 expression is reduced in acute neuroinflammatory conditions both in vivo and in vitro. A, A decreases CD200 protein expression in vivo as assessed by Western blot analysis(*p0.05;n12).B,FluorescentimagesofCD200inthedentategyrusofcontrol(i) and A-treated (ii) animals. C, A treatment increases MHCII mRNA expression (***p 0.001;n20).D,FluorescentimagesofMHCIIincontrol(i)andA-treated(ii)animals.E,A decreasesCD200proteinexpressioninvitroasdeterminedbyWesternblotanalysis(*p0.05; n 6). F, Fluorescent images of CD200 in the control (i) and A-treated (ii) neurons. G, A treatment increases MHCII mRNA expression in vitro (***p 0.001; n 4). H, Fluorescent images of microglia in the control (i) and A-treated (ii) cells. Scale bars, 20 M. Error bars indicate SEM.

Article Snippet: Cells and sections were treated overnight at 4°C as follows: mouse monoclonal CD200 antibody (1:200; Abcam, Cambridge, UK), mouse monoclonal MHCII antibody (1:100; Serotec), goat polyclonal CD200R antibody (1:100; Santa Cruz Biotechnology), and mouse monoclonal III-tubulin antibody (1:200; Chemicon).

Techniques: Expressing, In Vivo, In Vitro, Western Blot, Control

Journal: Journal of Neuroscience

Article Title: CD200 Ligand Receptor Interaction Modulates Microglial Activation In Vivo and In Vitro: A Role for IL-4

doi: 10.1523/jneurosci.1781-07.2007

Figure Lengend Snippet: Figure4. IL-4increasesneuronalCD200expression.A,IL-4increasesCD200proteinexpres- sion in cultured neurons by Western blot (***p 0.001). B, Fluorescent images of CD200 expression in cultured neurons in the absence (i) and presence (ii) of IL-4. C, Intracerebroven- tricular injection of IL-4 increases CD200 protein expression in hippocampus (*p 0.05; n 3).D,FluorescentimagesofincreasedCD200expressioninhippocampusofIL-4-treatedrats(ii) versuscontrol(i).E,CD200expressionissignificantlydecreasedinhippocampaltissueprepared from IL-4 / mice (*p 0.05; n 4). F, CD200 expression is significantly decreased in cultured neurons prepared from IL-4 / mice (ii) compared with neurons prepared from wild-type mice (i). G, MHCII mRNA expression is increased in cultured glial cells prepared from IL-4 / mice (*p 0.05; n 5). H, IL-1 concentration is increased in cultured glial cells prepared from IL-4 / mice (**p 0.01; n 10). Scale bars, 20 M. Error bars indicate SEM. WT, Wild type.

Article Snippet: Cells and sections were treated overnight at 4°C as follows: mouse monoclonal CD200 antibody (1:200; Abcam, Cambridge, UK), mouse monoclonal MHCII antibody (1:100; Serotec), goat polyclonal CD200R antibody (1:100; Santa Cruz Biotechnology), and mouse monoclonal III-tubulin antibody (1:200; Chemicon).

Techniques: Cell Culture, Western Blot, Expressing, Injection, Concentration Assay

Journal: Journal of Neuroscience

Article Title: Vascular Remodeling versus Amyloid -Induced Oxidative Stress in the Cerebrovascular Dysfunctions Associated with Alzheimer's Disease

doi: 10.1523/jneurosci.4031-05.2005

Figure Lengend Snippet: Figure6. NitrosativestressinthecerebralcortexofAPP andTGF mice.Nitrotyrosineimmunoreactivityisupregulatedin the vessel walls of young APP mice, whereas it is distributed in the neuropil and around intraparenchymal blood vessels in 12-month-old APP mice. As shown in the adjacent panels, nitrotyrosine immunoreactivity (red) is mainly located in CD11b- immunopositive microglial processes (green; the merge with nitrotyrosine is yellow) and cells (open arrows), but some neurons are also nitrotyrosine immunopositive (small arrows). In TGF mice, nitrotyrosine immunoreactivity is primarily distributed to glial elements in both wild-type (WT) and transgenic mice. Scale bar, 75 m. mo, Month.

Article Snippet: Changes in pial and/or intracortical microvessels in transgenic mice (perfusion-fixed brains) and AD patients (50- m-thick sections) were assessed by immunostaining for the following markers: SOD2 (rabbit anti-MnSOD; 1:400; Stressgen), nitrosative stress (mouse anti-nitrotyrosine; 1:1000; Upstate Biotechnology, Lake Placid, NY), basement membrane proteins collagen-I (goat anti- collagen type I; 1:400; Southern Biotechnology, Birmingham, AL) and collagen-IV (goat anti-collagen type IV; 1: 400; Chemicon), and astroglial (GFAP, rabbit anti-GFAP; 1:300; Dako, Glostrup, Denmark) or microglial (rat anti-CD11b; 1:100; Serotec, Raleigh, NC) activation.

Techniques: Transgenic Assay