Journal: Dentistry Journal

Article Title: Investigation of the Molecular Profile of Granular Cell Tumours and Schwannomas of the Oral Cavity

doi: 10.3390/dj10030038

Figure Lengend Snippet: Summary of the antibodies used for immunohistochemistry of GCTs and schwannomas.

Article Snippet: GAP43 , Bio-Techne Canada, Oakville On, NB300-143 , Rabbit polyclonal , Regenerating neural tissues/growth cones, GAP43 intracellular growth protein/membrane protein , 1/5000.

Techniques: Immunohistochemistry, Cell Surface Receptor Assay, Membrane, Plasmid Preparation

Journal: Neuroscience letters

Article Title: Overexpression of miR-338-5p in exosomes derived from mesenchymal stromal cells provides neuroprotective effects by the Cnr1/Rap1/Akt pathway after spinal cord injury in rats.

doi: 10.1016/j.neulet.2021.136124

Figure Lengend Snippet: Fig. 3. The expression of miR-338-5p was lower in the rats with SCI than the control rats, and the miR- 338-5p-overexpressing exosomes provided neuro protective effects after SCI. (A) Expression of miR- 338-5p was assessed by qRT-PCR at 1, 4 and 7 days after acute spinal cord injury in rats. (B, C) Western blot analysis of NF-M, GAP43, MAG and GFAP levels following various treatments at day 4 post-SCI. Data are presented as the mean ± SD. n = 4 per group (A), n = 3 per group (C), #p < 0.05, ##p < 0.01, *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: After the membranes were blocked with 5% nonfat milk in TBST for 1 h at room temperature, they were incubated with primary antibodies overnight at 4◦C as follows: TSG101 (1:1000, Santa Cruz Biotechnology), HSP70 (1:1000, Santa Cruz Biotechnology), CD63 (1:1000, Santa Cruz Biotechnology), CD9 (1:1000, Santa Cruz Biotechnology), Cnr1 (1:1000, Santa Cruz Biotechnology), Rap1 (1:1000, Santa Cruz Biotechnology), neurofilament-M (NF-M) (1:1000, Proteintech Group), growth associated protein-43 (GAP43) (1:1000, Santa Cruz Biotechnology), myelinassociated glycoprotein (MAG) (1:1000, Proteintech Group), glial fibrillary acidic protein (GFAP) (1:1000, Proteintech Group), Bax (1:1000, Cell Signaling Technology), Bcl-2 (1:1000, Cell Signaling Technology), pro caspase-3 (1:1000, Cell Signaling Technology), cleaved-caspase-3 (1:1000, Cell Signaling Technology), total-PI3K (1:1000, Cell Signaling Technology), p-PI3K (1:1000, Cell Signaling Technology), total-AKT (1:1000, Cell Signaling Technology), and p-AKT (1:1000, Cell Signaling Technology).

Techniques: Expressing, Control, Quantitative RT-PCR, Western Blot

Journal: Neuroscience letters

Article Title: Overexpression of miR-338-5p in exosomes derived from mesenchymal stromal cells provides neuroprotective effects by the Cnr1/Rap1/Akt pathway after spinal cord injury in rats.

doi: 10.1016/j.neulet.2021.136124

Figure Lengend Snippet: Fig. 4. Immunofluorescence double staining of NF-M, GAP43, MAG and GFAP levels following various treatments at day 4 post-SCI in rats. (A) Increased levels of the NF-M and GAP43 proteins in the region of anterior horn and adjacent lateral funiculus were observed in the miR-338-5p exo group compared to the other SCI groups at day 4 post-SCI. (B) Decreased protein levels of MAG and GFAP in the region of anterior horn and adjacent lateral funiculus were observed in the miR-338-5p exo group compared to the other SCI groups at day 4 post-SCI. Scale bar, 200 µm. (C) Semi-quantification of the mean fluorescence intensity of NF-M, GAP43, MAG and GFAP were measured. Data are presented as the mean ± SD. n = 3 per group, #p < 0.05, ##p < 0.01, ###p < 0.001, *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: After the membranes were blocked with 5% nonfat milk in TBST for 1 h at room temperature, they were incubated with primary antibodies overnight at 4◦C as follows: TSG101 (1:1000, Santa Cruz Biotechnology), HSP70 (1:1000, Santa Cruz Biotechnology), CD63 (1:1000, Santa Cruz Biotechnology), CD9 (1:1000, Santa Cruz Biotechnology), Cnr1 (1:1000, Santa Cruz Biotechnology), Rap1 (1:1000, Santa Cruz Biotechnology), neurofilament-M (NF-M) (1:1000, Proteintech Group), growth associated protein-43 (GAP43) (1:1000, Santa Cruz Biotechnology), myelinassociated glycoprotein (MAG) (1:1000, Proteintech Group), glial fibrillary acidic protein (GFAP) (1:1000, Proteintech Group), Bax (1:1000, Cell Signaling Technology), Bcl-2 (1:1000, Cell Signaling Technology), pro caspase-3 (1:1000, Cell Signaling Technology), cleaved-caspase-3 (1:1000, Cell Signaling Technology), total-PI3K (1:1000, Cell Signaling Technology), p-PI3K (1:1000, Cell Signaling Technology), total-AKT (1:1000, Cell Signaling Technology), and p-AKT (1:1000, Cell Signaling Technology).

Techniques: Immunofluorescence, Double Staining, Fluorescence

Journal: Neuroscience letters

Article Title: Overexpression of miR-338-5p in exosomes derived from mesenchymal stromal cells provides neuroprotective effects by the Cnr1/Rap1/Akt pathway after spinal cord injury in rats.

doi: 10.1016/j.neulet.2021.136124

Figure Lengend Snippet: Fig. 6. Overexpression of miR-338-5p alleviated H2O2-induced cell injury in PC12 cells. (A) PC12 cells were transfected with NC mimics/inhibitors, miR-338-5p mimics and inhibitors. qRT-PCR analysis of the miR-338-5p level. (B, C) Measurement of intracellular ROS and SOD. (D) CCK-8 assay for cell viability. (E, F) Western blot analysis of NF-M, GAP43, MAG and GFAP levels following various treatments in PC12 cells. n = 3 per group, #p < 0.05, ##p < 0.01, ###p < 0.001, *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: After the membranes were blocked with 5% nonfat milk in TBST for 1 h at room temperature, they were incubated with primary antibodies overnight at 4◦C as follows: TSG101 (1:1000, Santa Cruz Biotechnology), HSP70 (1:1000, Santa Cruz Biotechnology), CD63 (1:1000, Santa Cruz Biotechnology), CD9 (1:1000, Santa Cruz Biotechnology), Cnr1 (1:1000, Santa Cruz Biotechnology), Rap1 (1:1000, Santa Cruz Biotechnology), neurofilament-M (NF-M) (1:1000, Proteintech Group), growth associated protein-43 (GAP43) (1:1000, Santa Cruz Biotechnology), myelinassociated glycoprotein (MAG) (1:1000, Proteintech Group), glial fibrillary acidic protein (GFAP) (1:1000, Proteintech Group), Bax (1:1000, Cell Signaling Technology), Bcl-2 (1:1000, Cell Signaling Technology), pro caspase-3 (1:1000, Cell Signaling Technology), cleaved-caspase-3 (1:1000, Cell Signaling Technology), total-PI3K (1:1000, Cell Signaling Technology), p-PI3K (1:1000, Cell Signaling Technology), total-AKT (1:1000, Cell Signaling Technology), and p-AKT (1:1000, Cell Signaling Technology).

Techniques: Over Expression, Transfection, Quantitative RT-PCR, CCK-8 Assay, Western Blot

Journal: Scientific Reports

Article Title: Expression and regulation of FRMD6 in mouse DRG neurons and spinal cord after nerve injury

doi: 10.1038/s41598-020-58261-7

Figure Lengend Snippet: Primary antibodies.

Article Snippet: GAP43 , Rabbit , IHC (Coons) , 1 μg/ml , Atlas Antibodies AB/HPA015600.

Techniques: