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Image Search Results
Journal: Investigative ophthalmology & visual science
Article Title: Three-Dimensional Neurite Characterization of Small Incision Lenticule Extraction Derived Lenticules.
doi: 10.1167/iovs.19-27566
Figure Lengend Snippet: FIGURE 4. Interaction between lenticule neurites and SCs. Colocaliza- tion of SC marker expression and stromal neurites by confocal immunofluorescence: (A–C) GAP43 (green) and TuJ1 (red). (D–F) p75NTR (green) and TuJ1 (red).
Article Snippet: Antibodies Used in This Study Antibody Source Working Concentration 1 bIII-tubulin Covance MMS435P 0.5 lg/mL 2
Techniques: Marker, Expressing
Journal: Translational Vision Science & Technology
Article Title: Tissue Responses and Wound Healing following Laser Scleral Microporation for Presbyopia Therapy
doi: 10.1167/tvst.9.4.6
Figure Lengend Snippet: Bar graph showing that the quantification of positively staining cells of the L + C group had significantly less CD11b and CD45-positive cells than L group at 1 month (A; * P < 0.05). The evaluation of staining intensity of tenascin-C, fibronectin, α-SMA, HSP47, and GAP43 by semiquantifying the mean gray value of the intensity for the L and L + C groups at different time points (B). * P < 0.05.
Article Snippet: The following primary antibodies were then added and incubated for 2 hours at room temperature: mouse monoclonal antibody against cellular fibronectin (MAB-1940; Millipore, Billerica, MA, USA) diluted 1∶100, tenascin-C (SC-20932; Santa Cruz Biotechnology, Dallas, TA, USA) diluted 1∶200, mouse monoclonal antibody against heat shock protein 47 (HSP47) (ADI-SPA-470; Enzo Life Sciences, Lausen, Switzerland) diluted 1:200, rabbit polyclonal antibody against CD11b (AB-133357; Abcam, Cambridge, UK) diluted 1:100, mouse monoclonal antibody against α–smooth muscle actin (α-SMA) (M0851; Dako, Glostrup, Denmark) diluted 1:100, mouse monoclonal antibody against CD45 (Ab-10558; Abcam) diluted 1:100, mouse monoclonal antibody against CD31 (Ab-199012; Abcam) diluted 1:100, mouse monoclonal antibody against CD90 (LS-B3139; LifeSpan Biosciences, CA, USA) diluted 1:100 and growth-associated
Techniques: Staining
Journal: Translational Vision Science & Technology
Article Title: Tissue Responses and Wound Healing following Laser Scleral Microporation for Presbyopia Therapy
doi: 10.1167/tvst.9.4.6
Figure Lengend Snippet: Expression of GAP43 and CD31 for the L and L + C groups at different time points. GAP43 was significantly upregulated with comparable staining intensity for both groups, and it resolved with time (A). Expression of CD31 was also observed around the microspores in all eyes at all time points (B). Nuclei were counterstained with DAPI ( blue ). Original magnification: 100×. Scale bar : 500 µm.
Article Snippet: The following primary antibodies were then added and incubated for 2 hours at room temperature: mouse monoclonal antibody against cellular fibronectin (MAB-1940; Millipore, Billerica, MA, USA) diluted 1∶100, tenascin-C (SC-20932; Santa Cruz Biotechnology, Dallas, TA, USA) diluted 1∶200, mouse monoclonal antibody against heat shock protein 47 (HSP47) (ADI-SPA-470; Enzo Life Sciences, Lausen, Switzerland) diluted 1:200, rabbit polyclonal antibody against CD11b (AB-133357; Abcam, Cambridge, UK) diluted 1:100, mouse monoclonal antibody against α–smooth muscle actin (α-SMA) (M0851; Dako, Glostrup, Denmark) diluted 1:100, mouse monoclonal antibody against CD45 (Ab-10558; Abcam) diluted 1:100, mouse monoclonal antibody against CD31 (Ab-199012; Abcam) diluted 1:100, mouse monoclonal antibody against CD90 (LS-B3139; LifeSpan Biosciences, CA, USA) diluted 1:100 and growth-associated
Techniques: Expressing, Staining