gabra1 Search Results


human gabaa receptor α1  (OriGene)
93
OriGene human gabaa receptor α1
Fig. 2. Whole-cell patch-clamp recording of selected eight <t>GABAA</t> receptor <t>α1</t> subunit DAVs. a HEK293T cells were transiently transfected with α1 (wild type or the indicated variants), β2, and γ2 cDNAs of GABAA receptors. Forty-eight hours post transfection, whole-cell currents were recorded using the IonFlux Mercury 16 ensemble plates at a holding voltage of −60 mV. Representative whole-cell voltage-clamp recording traces are shown. Application of GABA (100 μM, 3 s) is indicated by the horizontal bar above the current traces. Quantification of the peak currents (Imax) is shown on the right (n = 4–10). nA: nano Ampere. b Dose-response curves of GABA are shown for the calculation of EC50 values for wild type and the indicated α1 DAVs (n = 3–4). Each data point is reported as mean ± SEM. One-way ANOVA followed by post-hoc Tukey test was used for statistical analysis. Statistical significance was labelled for the comparison to the WT group. ***, p < 0.001.
Human Gabaa Receptor α1, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gabra1/pm39216838-68-4-44?v=OriGene
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human alpha 1 subunit  (OriGene)
90
OriGene human alpha 1 subunit
Fig. 2. Whole-cell patch-clamp recording of selected eight <t>GABAA</t> receptor <t>α1</t> subunit DAVs. a HEK293T cells were transiently transfected with α1 (wild type or the indicated variants), β2, and γ2 cDNAs of GABAA receptors. Forty-eight hours post transfection, whole-cell currents were recorded using the IonFlux Mercury 16 ensemble plates at a holding voltage of −60 mV. Representative whole-cell voltage-clamp recording traces are shown. Application of GABA (100 μM, 3 s) is indicated by the horizontal bar above the current traces. Quantification of the peak currents (Imax) is shown on the right (n = 4–10). nA: nano Ampere. b Dose-response curves of GABA are shown for the calculation of EC50 values for wild type and the indicated α1 DAVs (n = 3–4). Each data point is reported as mean ± SEM. One-way ANOVA followed by post-hoc Tukey test was used for statistical analysis. Statistical significance was labelled for the comparison to the WT group. ***, p < 0.001.
Human Alpha 1 Subunit, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human gabaa receptors  (OriGene)
90
OriGene human gabaa receptors
Fig. 2. Whole-cell patch-clamp recording of selected eight <t>GABAA</t> receptor <t>α1</t> subunit DAVs. a HEK293T cells were transiently transfected with α1 (wild type or the indicated variants), β2, and γ2 cDNAs of GABAA receptors. Forty-eight hours post transfection, whole-cell currents were recorded using the IonFlux Mercury 16 ensemble plates at a holding voltage of −60 mV. Representative whole-cell voltage-clamp recording traces are shown. Application of GABA (100 μM, 3 s) is indicated by the horizontal bar above the current traces. Quantification of the peak currents (Imax) is shown on the right (n = 4–10). nA: nano Ampere. b Dose-response curves of GABA are shown for the calculation of EC50 values for wild type and the indicated α1 DAVs (n = 3–4). Each data point is reported as mean ± SEM. One-way ANOVA followed by post-hoc Tukey test was used for statistical analysis. Statistical significance was labelled for the comparison to the WT group. ***, p < 0.001.
Human Gabaa Receptors, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gabra1  (Proteintech)
93
Proteintech gabra1
Fig. 2. Whole-cell patch-clamp recording of selected eight <t>GABAA</t> receptor <t>α1</t> subunit DAVs. a HEK293T cells were transiently transfected with α1 (wild type or the indicated variants), β2, and γ2 cDNAs of GABAA receptors. Forty-eight hours post transfection, whole-cell currents were recorded using the IonFlux Mercury 16 ensemble plates at a holding voltage of −60 mV. Representative whole-cell voltage-clamp recording traces are shown. Application of GABA (100 μM, 3 s) is indicated by the horizontal bar above the current traces. Quantification of the peak currents (Imax) is shown on the right (n = 4–10). nA: nano Ampere. b Dose-response curves of GABA are shown for the calculation of EC50 values for wild type and the indicated α1 DAVs (n = 3–4). Each data point is reported as mean ± SEM. One-way ANOVA followed by post-hoc Tukey test was used for statistical analysis. Statistical significance was labelled for the comparison to the WT group. ***, p < 0.001.
Gabra1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gabra1/pm41754806-179-36-37?v=Proteintech
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rabbit anti gabaa α1 receptor  (Alomone Labs)
94
Alomone Labs rabbit anti gabaa α1 receptor
Fig. 2. Whole-cell patch-clamp recording of selected eight <t>GABAA</t> receptor <t>α1</t> subunit DAVs. a HEK293T cells were transiently transfected with α1 (wild type or the indicated variants), β2, and γ2 cDNAs of GABAA receptors. Forty-eight hours post transfection, whole-cell currents were recorded using the IonFlux Mercury 16 ensemble plates at a holding voltage of −60 mV. Representative whole-cell voltage-clamp recording traces are shown. Application of GABA (100 μM, 3 s) is indicated by the horizontal bar above the current traces. Quantification of the peak currents (Imax) is shown on the right (n = 4–10). nA: nano Ampere. b Dose-response curves of GABA are shown for the calculation of EC50 values for wild type and the indicated α1 DAVs (n = 3–4). Each data point is reported as mean ± SEM. One-way ANOVA followed by post-hoc Tukey test was used for statistical analysis. Statistical significance was labelled for the comparison to the WT group. ***, p < 0.001.
Rabbit Anti Gabaa α1 Receptor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gabra1/pm26365992-155-41-47?v=Alomone+Labs
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gene exp gabra1 hs00168058 m1  (Thermo Fisher)
85
Thermo Fisher gene exp gabra1 hs00168058 m1
Fig. 2. Whole-cell patch-clamp recording of selected eight <t>GABAA</t> receptor <t>α1</t> subunit DAVs. a HEK293T cells were transiently transfected with α1 (wild type or the indicated variants), β2, and γ2 cDNAs of GABAA receptors. Forty-eight hours post transfection, whole-cell currents were recorded using the IonFlux Mercury 16 ensemble plates at a holding voltage of −60 mV. Representative whole-cell voltage-clamp recording traces are shown. Application of GABA (100 μM, 3 s) is indicated by the horizontal bar above the current traces. Quantification of the peak currents (Imax) is shown on the right (n = 4–10). nA: nano Ampere. b Dose-response curves of GABA are shown for the calculation of EC50 values for wild type and the indicated α1 DAVs (n = 3–4). Each data point is reported as mean ± SEM. One-way ANOVA followed by post-hoc Tukey test was used for statistical analysis. Statistical significance was labelled for the comparison to the WT group. ***, p < 0.001.
Gene Exp Gabra1 Hs00168058 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp gabra1 rn00788315 m1  (Thermo Fisher)
94
Thermo Fisher gene exp gabra1 rn00788315 m1
Fig. 2. Whole-cell patch-clamp recording of selected eight <t>GABAA</t> receptor <t>α1</t> subunit DAVs. a HEK293T cells were transiently transfected with α1 (wild type or the indicated variants), β2, and γ2 cDNAs of GABAA receptors. Forty-eight hours post transfection, whole-cell currents were recorded using the IonFlux Mercury 16 ensemble plates at a holding voltage of −60 mV. Representative whole-cell voltage-clamp recording traces are shown. Application of GABA (100 μM, 3 s) is indicated by the horizontal bar above the current traces. Quantification of the peak currents (Imax) is shown on the right (n = 4–10). nA: nano Ampere. b Dose-response curves of GABA are shown for the calculation of EC50 values for wild type and the indicated α1 DAVs (n = 3–4). Each data point is reported as mean ± SEM. One-way ANOVA followed by post-hoc Tukey test was used for statistical analysis. Statistical significance was labelled for the comparison to the WT group. ***, p < 0.001.
Gene Exp Gabra1 Rn00788315 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gabra1/pmc06231992-0-6--1?v=Thermo+Fisher
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gene exp gabra1 rn00788315 m1 - by Bioz Stars, 2026-08
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gene exp gabra1 hs00971228 m1  (Thermo Fisher)
87
Thermo Fisher gene exp gabra1 hs00971228 m1
Fig. 2. Whole-cell patch-clamp recording of selected eight <t>GABAA</t> receptor <t>α1</t> subunit DAVs. a HEK293T cells were transiently transfected with α1 (wild type or the indicated variants), β2, and γ2 cDNAs of GABAA receptors. Forty-eight hours post transfection, whole-cell currents were recorded using the IonFlux Mercury 16 ensemble plates at a holding voltage of −60 mV. Representative whole-cell voltage-clamp recording traces are shown. Application of GABA (100 μM, 3 s) is indicated by the horizontal bar above the current traces. Quantification of the peak currents (Imax) is shown on the right (n = 4–10). nA: nano Ampere. b Dose-response curves of GABA are shown for the calculation of EC50 values for wild type and the indicated α1 DAVs (n = 3–4). Each data point is reported as mean ± SEM. One-way ANOVA followed by post-hoc Tukey test was used for statistical analysis. Statistical significance was labelled for the comparison to the WT group. ***, p < 0.001.
Gene Exp Gabra1 Hs00971228 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp gabra1 mm01224902 m1  (Thermo Fisher)
91
Thermo Fisher gene exp gabra1 mm01224902 m1
Fig. 2. Whole-cell patch-clamp recording of selected eight <t>GABAA</t> receptor <t>α1</t> subunit DAVs. a HEK293T cells were transiently transfected with α1 (wild type or the indicated variants), β2, and γ2 cDNAs of GABAA receptors. Forty-eight hours post transfection, whole-cell currents were recorded using the IonFlux Mercury 16 ensemble plates at a holding voltage of −60 mV. Representative whole-cell voltage-clamp recording traces are shown. Application of GABA (100 μM, 3 s) is indicated by the horizontal bar above the current traces. Quantification of the peak currents (Imax) is shown on the right (n = 4–10). nA: nano Ampere. b Dose-response curves of GABA are shown for the calculation of EC50 values for wild type and the indicated α1 DAVs (n = 3–4). Each data point is reported as mean ± SEM. One-way ANOVA followed by post-hoc Tukey test was used for statistical analysis. Statistical significance was labelled for the comparison to the WT group. ***, p < 0.001.
Gene Exp Gabra1 Mm01224902 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gabra1/pmc08898592__NIHMS1781185___supplement___1-64-29--1?v=Thermo+Fisher
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gene exp gabra1 mm00439046 m1  (Thermo Fisher)
98
Thermo Fisher gene exp gabra1 mm00439046 m1
Fig. 2. Whole-cell patch-clamp recording of selected eight <t>GABAA</t> receptor <t>α1</t> subunit DAVs. a HEK293T cells were transiently transfected with α1 (wild type or the indicated variants), β2, and γ2 cDNAs of GABAA receptors. Forty-eight hours post transfection, whole-cell currents were recorded using the IonFlux Mercury 16 ensemble plates at a holding voltage of −60 mV. Representative whole-cell voltage-clamp recording traces are shown. Application of GABA (100 μM, 3 s) is indicated by the horizontal bar above the current traces. Quantification of the peak currents (Imax) is shown on the right (n = 4–10). nA: nano Ampere. b Dose-response curves of GABA are shown for the calculation of EC50 values for wild type and the indicated α1 DAVs (n = 3–4). Each data point is reported as mean ± SEM. One-way ANOVA followed by post-hoc Tukey test was used for statistical analysis. Statistical significance was labelled for the comparison to the WT group. ***, p < 0.001.
Gene Exp Gabra1 Mm00439046 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gabra1/pmc03607152-68-19-11?v=Thermo+Fisher
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guinea pig anti gabaar alpha 1 subunit  (Alomone Labs)
92
Alomone Labs guinea pig anti gabaar alpha 1 subunit
Fig. 2. Whole-cell patch-clamp recording of selected eight <t>GABAA</t> receptor <t>α1</t> subunit DAVs. a HEK293T cells were transiently transfected with α1 (wild type or the indicated variants), β2, and γ2 cDNAs of GABAA receptors. Forty-eight hours post transfection, whole-cell currents were recorded using the IonFlux Mercury 16 ensemble plates at a holding voltage of −60 mV. Representative whole-cell voltage-clamp recording traces are shown. Application of GABA (100 μM, 3 s) is indicated by the horizontal bar above the current traces. Quantification of the peak currents (Imax) is shown on the right (n = 4–10). nA: nano Ampere. b Dose-response curves of GABA are shown for the calculation of EC50 values for wild type and the indicated α1 DAVs (n = 3–4). Each data point is reported as mean ± SEM. One-way ANOVA followed by post-hoc Tukey test was used for statistical analysis. Statistical significance was labelled for the comparison to the WT group. ***, p < 0.001.
Guinea Pig Anti Gabaar Alpha 1 Subunit, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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neurons  (Biorbyt)
91
Biorbyt neurons
Fig. 2. Whole-cell patch-clamp recording of selected eight <t>GABAA</t> receptor <t>α1</t> subunit DAVs. a HEK293T cells were transiently transfected with α1 (wild type or the indicated variants), β2, and γ2 cDNAs of GABAA receptors. Forty-eight hours post transfection, whole-cell currents were recorded using the IonFlux Mercury 16 ensemble plates at a holding voltage of −60 mV. Representative whole-cell voltage-clamp recording traces are shown. Application of GABA (100 μM, 3 s) is indicated by the horizontal bar above the current traces. Quantification of the peak currents (Imax) is shown on the right (n = 4–10). nA: nano Ampere. b Dose-response curves of GABA are shown for the calculation of EC50 values for wild type and the indicated α1 DAVs (n = 3–4). Each data point is reported as mean ± SEM. One-way ANOVA followed by post-hoc Tukey test was used for statistical analysis. Statistical significance was labelled for the comparison to the WT group. ***, p < 0.001.
Neurons, supplied by Biorbyt, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 2. Whole-cell patch-clamp recording of selected eight GABAA receptor α1 subunit DAVs. a HEK293T cells were transiently transfected with α1 (wild type or the indicated variants), β2, and γ2 cDNAs of GABAA receptors. Forty-eight hours post transfection, whole-cell currents were recorded using the IonFlux Mercury 16 ensemble plates at a holding voltage of −60 mV. Representative whole-cell voltage-clamp recording traces are shown. Application of GABA (100 μM, 3 s) is indicated by the horizontal bar above the current traces. Quantification of the peak currents (Imax) is shown on the right (n = 4–10). nA: nano Ampere. b Dose-response curves of GABA are shown for the calculation of EC50 values for wild type and the indicated α1 DAVs (n = 3–4). Each data point is reported as mean ± SEM. One-way ANOVA followed by post-hoc Tukey test was used for statistical analysis. Statistical significance was labelled for the comparison to the WT group. ***, p < 0.001.

Journal: Pharmacological research

Article Title: Pharmacological chaperones restore proteostasis of epilepsy-associated GABA A receptor variants.

doi: 10.1016/j.phrs.2024.107356

Figure Lengend Snippet: Fig. 2. Whole-cell patch-clamp recording of selected eight GABAA receptor α1 subunit DAVs. a HEK293T cells were transiently transfected with α1 (wild type or the indicated variants), β2, and γ2 cDNAs of GABAA receptors. Forty-eight hours post transfection, whole-cell currents were recorded using the IonFlux Mercury 16 ensemble plates at a holding voltage of −60 mV. Representative whole-cell voltage-clamp recording traces are shown. Application of GABA (100 μM, 3 s) is indicated by the horizontal bar above the current traces. Quantification of the peak currents (Imax) is shown on the right (n = 4–10). nA: nano Ampere. b Dose-response curves of GABA are shown for the calculation of EC50 values for wild type and the indicated α1 DAVs (n = 3–4). Each data point is reported as mean ± SEM. One-way ANOVA followed by post-hoc Tukey test was used for statistical analysis. Statistical significance was labelled for the comparison to the WT group. ***, p < 0.001.

Article Snippet: The pCMV6 plasmids containing human GABAA receptor α1 (Uniprot no. P14867–1) (catalog #: RC205390), β2 (isoform 2, Uniprot no. P47870–1) (catalog #: RC216424), γ2 (isoform 2, Uniprot no. P18507–2) (catalog #: RC209260), and pCMV6 Entry Vector plasmid (pCMV6-EV) (catalog #: PS100001) were obtained from Origene.

Techniques: Patch Clamp, Transfection, Comparison

Fig. 4. Hispidulin and TP003 promote the functional surface expression of α1 DAVs. a Effect of Hispidulin (10 µM, 24 h) or TP003 (5 µM, 24 h) on the surface protein expression of the α1 variants in HEK293T cells expressing α1(A322D)β2γ2, α1(D219N)β2γ2, α1(P260L)β2γ2, α1(R214C)β2γ2, α1(S76R)β2γ2, α1(G251D)β2γ2, α1 (M263T)β2γ2, or α1(T289P)β2γ2 GABAA receptors according to surface biotinylation analysis. Na+/K+ ATPase serves as a plasma membrane protein loading control. Quantification of the surface α1 band intensities was shown on the bottom panels (n = 3). b Effect of Hispidulin (10 µM, 24 h) or TP003 (5 µM, 24 h) on GABA- induced peak current amplitudes in HEK293T cells expressing α1(A322D)β2γ2, α1(D219N)β2γ2, α1(P260L)β2γ2, α1(R214C)β2γ2, α1(S76R)β2γ2, α1(G251D)β2γ2, α1(M263T)β2γ2, or α1(T289P)β2γ2 GABAA receptors. Representative whole-cell voltage-clamp recording traces are shown. The holding voltage was set at −60 mV. Application of GABA (100 μM, 3 s) is indicated by the horizontal bar above the current traces. Quantification of the peak currents (Imax) are shown on the bottom panels (n = 4–11). pA: pico Ampere. c Comparison of the peak current amplitudes of compound (DMSO, Hispidulin, or TP003)-treated α1 variants with those in wild type (WT) receptors. Data was taken from Fig. 2a and Fig. 4b. Each data point is reported as mean ± SEM. One-way ANOVA followed by post-hoc Tukey test was used for statistical analysis. Statistical significance was labelled for the comparison to the DMSO group ((a, b) or WT group (a, b) or WT group (c). NS, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001. Also see Supplementary Fig. S5.

Journal: Pharmacological research

Article Title: Pharmacological chaperones restore proteostasis of epilepsy-associated GABA A receptor variants.

doi: 10.1016/j.phrs.2024.107356

Figure Lengend Snippet: Fig. 4. Hispidulin and TP003 promote the functional surface expression of α1 DAVs. a Effect of Hispidulin (10 µM, 24 h) or TP003 (5 µM, 24 h) on the surface protein expression of the α1 variants in HEK293T cells expressing α1(A322D)β2γ2, α1(D219N)β2γ2, α1(P260L)β2γ2, α1(R214C)β2γ2, α1(S76R)β2γ2, α1(G251D)β2γ2, α1 (M263T)β2γ2, or α1(T289P)β2γ2 GABAA receptors according to surface biotinylation analysis. Na+/K+ ATPase serves as a plasma membrane protein loading control. Quantification of the surface α1 band intensities was shown on the bottom panels (n = 3). b Effect of Hispidulin (10 µM, 24 h) or TP003 (5 µM, 24 h) on GABA- induced peak current amplitudes in HEK293T cells expressing α1(A322D)β2γ2, α1(D219N)β2γ2, α1(P260L)β2γ2, α1(R214C)β2γ2, α1(S76R)β2γ2, α1(G251D)β2γ2, α1(M263T)β2γ2, or α1(T289P)β2γ2 GABAA receptors. Representative whole-cell voltage-clamp recording traces are shown. The holding voltage was set at −60 mV. Application of GABA (100 μM, 3 s) is indicated by the horizontal bar above the current traces. Quantification of the peak currents (Imax) are shown on the bottom panels (n = 4–11). pA: pico Ampere. c Comparison of the peak current amplitudes of compound (DMSO, Hispidulin, or TP003)-treated α1 variants with those in wild type (WT) receptors. Data was taken from Fig. 2a and Fig. 4b. Each data point is reported as mean ± SEM. One-way ANOVA followed by post-hoc Tukey test was used for statistical analysis. Statistical significance was labelled for the comparison to the DMSO group ((a, b) or WT group (a, b) or WT group (c). NS, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001. Also see Supplementary Fig. S5.

Article Snippet: The pCMV6 plasmids containing human GABAA receptor α1 (Uniprot no. P14867–1) (catalog #: RC205390), β2 (isoform 2, Uniprot no. P47870–1) (catalog #: RC216424), γ2 (isoform 2, Uniprot no. P18507–2) (catalog #: RC209260), and pCMV6 Entry Vector plasmid (pCMV6-EV) (catalog #: PS100001) were obtained from Origene.

Techniques: Functional Assay, Expressing, Clinical Proteomics, Membrane, Control, Comparison

Fig. 8. Proposed mechanism of action of Hispidulin and TP003 on rescuing α1 DAV trafficking. Hispidulin and TP003 act as pharmacological chaperones to bind GABAA receptor variants to stabilize them in the ER. Pharmacological chaperone treatment enhances the assembly of the variants and their forward trafficking to the Golgi and plasma membrane. The effect of Hispidulin and TP003 is also reflected from the enhanced folding and reduced degradation of the variants. Consistently, the interactions between the variants and pro-folding chaperones (BiP and calnexin) and a trafficking factor (LMAN1) are enhanced, whereas the interactions be tween the variants and ERAD factors (Grp94 and VCP) are reduced by pharmacological chaperone treatment.

Journal: Pharmacological research

Article Title: Pharmacological chaperones restore proteostasis of epilepsy-associated GABA A receptor variants.

doi: 10.1016/j.phrs.2024.107356

Figure Lengend Snippet: Fig. 8. Proposed mechanism of action of Hispidulin and TP003 on rescuing α1 DAV trafficking. Hispidulin and TP003 act as pharmacological chaperones to bind GABAA receptor variants to stabilize them in the ER. Pharmacological chaperone treatment enhances the assembly of the variants and their forward trafficking to the Golgi and plasma membrane. The effect of Hispidulin and TP003 is also reflected from the enhanced folding and reduced degradation of the variants. Consistently, the interactions between the variants and pro-folding chaperones (BiP and calnexin) and a trafficking factor (LMAN1) are enhanced, whereas the interactions be tween the variants and ERAD factors (Grp94 and VCP) are reduced by pharmacological chaperone treatment.

Article Snippet: The pCMV6 plasmids containing human GABAA receptor α1 (Uniprot no. P14867–1) (catalog #: RC205390), β2 (isoform 2, Uniprot no. P47870–1) (catalog #: RC216424), γ2 (isoform 2, Uniprot no. P18507–2) (catalog #: RC209260), and pCMV6 Entry Vector plasmid (pCMV6-EV) (catalog #: PS100001) were obtained from Origene.

Techniques: Clinical Proteomics, Membrane