g043h7 Search Results


tcr γδ  (fluidigm)
93
fluidigm tcr γδ
Unsupervised clustering of epigenetic changes between and within the different mucosal cell subsets after wild-type S . Typhi infections. Cells isolated cells from healthy terminal ileum surgical tissues were exposed to S . Typhi strain Ty2 (Ty2) at 1:100 MOI. Cells cultured with media only were used as controls (media). After 3 h of incubation, cells were stained using a panel of 33 metal-labeled Abs, and the chromatin modifications were analyzed at the single-cell level by mass cytometry. To visualize the clustering of the 11 cell subsets ( i.e ., B-, CD3+ T-, CD4+ T-, CD8 + T-, NK, <t>TCR-γδ,</t> Mucosal associated invariant (MAIT) and NKT cells, as well as monocytes, macrophages, and epithelial cells), tSNE-defined population distributions and clustering were colored by meta-cluster. ( A ) t-SNE maps of chromatin modifications. Settings to run the t-SNE algorithm were set-up in Cytobank. ( B ) Color-coded key showing the location of epigenetic marks meta-clusters. Data are representative of one out of three experiments with terminal ileum segments from 2 different donors, one replicate each.
Tcr γδ, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/g043h7/pmc07423951-221-60-63?v=fluidigm
Average 93 stars, based on 1 article reviews
tcr γδ - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier
g043h7  (fluidigm)
93
fluidigm g043h7
Unsupervised clustering of epigenetic changes between and within the different mucosal cell subsets after wild-type S . Typhi infections. Cells isolated cells from healthy terminal ileum surgical tissues were exposed to S . Typhi strain Ty2 (Ty2) at 1:100 MOI. Cells cultured with media only were used as controls (media). After 3 h of incubation, cells were stained using a panel of 33 metal-labeled Abs, and the chromatin modifications were analyzed at the single-cell level by mass cytometry. To visualize the clustering of the 11 cell subsets ( i.e ., B-, CD3+ T-, CD4+ T-, CD8 + T-, NK, <t>TCR-γδ,</t> Mucosal associated invariant (MAIT) and NKT cells, as well as monocytes, macrophages, and epithelial cells), tSNE-defined population distributions and clustering were colored by meta-cluster. ( A ) t-SNE maps of chromatin modifications. Settings to run the t-SNE algorithm were set-up in Cytobank. ( B ) Color-coded key showing the location of epigenetic marks meta-clusters. Data are representative of one out of three experiments with terminal ileum segments from 2 different donors, one replicate each.
G043h7, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/g043h7/pmc08671535__41467_2021_27574_MOESM2_ESM-12-25-30?v=fluidigm
Average 93 stars, based on 1 article reviews
g043h7 - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier
cd197-fitc 5:100, clone g043h7 antibody  (Sony)
90
Sony cd197-fitc 5:100, clone g043h7 antibody
Unsupervised clustering of epigenetic changes between and within the different mucosal cell subsets after wild-type S . Typhi infections. Cells isolated cells from healthy terminal ileum surgical tissues were exposed to S . Typhi strain Ty2 (Ty2) at 1:100 MOI. Cells cultured with media only were used as controls (media). After 3 h of incubation, cells were stained using a panel of 33 metal-labeled Abs, and the chromatin modifications were analyzed at the single-cell level by mass cytometry. To visualize the clustering of the 11 cell subsets ( i.e ., B-, CD3+ T-, CD4+ T-, CD8 + T-, NK, <t>TCR-γδ,</t> Mucosal associated invariant (MAIT) and NKT cells, as well as monocytes, macrophages, and epithelial cells), tSNE-defined population distributions and clustering were colored by meta-cluster. ( A ) t-SNE maps of chromatin modifications. Settings to run the t-SNE algorithm were set-up in Cytobank. ( B ) Color-coded key showing the location of epigenetic marks meta-clusters. Data are representative of one out of three experiments with terminal ileum segments from 2 different donors, one replicate each.
Cd197 Fitc 5:100, Clone G043h7 Antibody, supplied by Sony, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/g043h7/pm37240393-371-58-84?v=Sony
Average 90 stars, based on 1 article reviews
cd197-fitc 5:100, clone g043h7 antibody - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
anti human cd197/ccr7 flow cytometry monoclonal clone g043h7 pe/cyanine5 ready to use  (Innovative Research Inc)
N/A
Anti Human CD197/CCR7 Flow Cytometry Monoclonal Clone G043H7 PE/Cyanine5 Ready To Use from Innovative Research is a flow cytometry antibody, buffered in PBS with 0.05% Proclin300, 1% BSA. This flow cytometry antibody has been specifically
   Buy from Supplier
anti human cd197/ccr7 flow cytometry monoclonal clone g043h7 apc ready to use  (Innovative Research Inc)
N/A
Anti Human CD197/CCR7 Flow Cytometry Monoclonal Clone G043H7 APC Ready To Use from Innovative Research is a flow cytometry antibody, buffered in PBS with 0.05% Proclin300, 1% BSA. This flow cytometry antibody has been specifically
   Buy from Supplier
anti human cd197/ccr7 flow cytometry monoclonal clone g043h7 fitc ready to use  (Innovative Research Inc)
N/A
Anti Human CD197/CCR7 Flow Cytometry Monoclonal Clone G043H7 FITC Ready To Use from Innovative Research is a flow cytometry antibody, buffered in PBS with 0.05% Proclin300, 1% BSA. This flow cytometry antibody has been specifically
   Buy from Supplier
anti human cd197/ccr7 flow cytometry monoclonal clone g043h7 percp/cyanine5.5 ready to use  (Innovative Research Inc)
N/A
Anti Human CD197/CCR7 Flow Cytometry Monoclonal Clone G043H7 PerCP/Cyanine5.5 Ready To Use from Innovative Research is a flow cytometry antibody, buffered in PBS with 0.05% Proclin300, 1% BSA. This flow cytometry antibody has been specifically
   Buy from Supplier
anti human cd197/ccr7 flow cytometry monoclonal clone g043h7 af647 ready to use  (Innovative Research Inc)
N/A
Anti Human CD197/CCR7 Flow Cytometry Monoclonal Clone G043H7 AF647 Ready To Use from Innovative Research is a flow cytometry antibody, buffered in PBS with 0.05% Proclin300, 1% BSA. This flow cytometry antibody has been specifically
   Buy from Supplier
anti human cd197/ccr7 flow cytometry monoclonal clone g043h7 ev450 ready to use  (Innovative Research Inc)
N/A
Anti Human CD197/CCR7 Flow Cytometry Monoclonal Clone G043H7 EV450 Ready To Use from Innovative Research is a flow cytometry antibody, buffered in PBS with 0.05% Proclin300, 1% BSA. This flow cytometry antibody has been specifically
   Buy from Supplier
anti human cd197/ccr7 flow cytometry monoclonal clone g043h7 af488 ready to use  (Innovative Research Inc)
N/A
Anti Human CD197/CCR7 Flow Cytometry Monoclonal Clone G043H7 AF488 Ready To Use from Innovative Research is a flow cytometry antibody, buffered in PBS with 0.05% Proclin300, 1% BSA. This flow cytometry antibody has been specifically
   Buy from Supplier
anti human cd197/ccr7 flow cytometry monoclonal clone g043h7 pe/cyanine7 ready to use  (Innovative Research Inc)
N/A
Anti Human CD197/CCR7 Flow Cytometry Monoclonal Clone G043H7 PE/Cyanine7 Ready To Use from Innovative Research is a flow cytometry antibody, buffered in PBS with 0.05% Proclin300, 1% BSA. This flow cytometry antibody has been specifically
   Buy from Supplier
anti human cd197/ccr7 flow cytometry monoclonal clone g043h7 pe/cyanine5.5 ready to use  (Innovative Research Inc)
N/A
Anti Human CD197/CCR7 Flow Cytometry Monoclonal Clone G043H7 PE/Cyanine5.5 Ready To Use from Innovative Research is a flow cytometry antibody, buffered in PBS with 0.05% Proclin300, 1% BSA. This flow cytometry antibody has been specifically
   Buy from Supplier

Image Search Results


Unsupervised clustering of epigenetic changes between and within the different mucosal cell subsets after wild-type S . Typhi infections. Cells isolated cells from healthy terminal ileum surgical tissues were exposed to S . Typhi strain Ty2 (Ty2) at 1:100 MOI. Cells cultured with media only were used as controls (media). After 3 h of incubation, cells were stained using a panel of 33 metal-labeled Abs, and the chromatin modifications were analyzed at the single-cell level by mass cytometry. To visualize the clustering of the 11 cell subsets ( i.e ., B-, CD3+ T-, CD4+ T-, CD8 + T-, NK, TCR-γδ, Mucosal associated invariant (MAIT) and NKT cells, as well as monocytes, macrophages, and epithelial cells), tSNE-defined population distributions and clustering were colored by meta-cluster. ( A ) t-SNE maps of chromatin modifications. Settings to run the t-SNE algorithm were set-up in Cytobank. ( B ) Color-coded key showing the location of epigenetic marks meta-clusters. Data are representative of one out of three experiments with terminal ileum segments from 2 different donors, one replicate each.

Journal: Scientific Reports

Article Title: Salmonella enterica serovar Typhi exposure elicits ex vivo cell-type-specific epigenetic changes in human gut cells

doi: 10.1038/s41598-020-70492-2

Figure Lengend Snippet: Unsupervised clustering of epigenetic changes between and within the different mucosal cell subsets after wild-type S . Typhi infections. Cells isolated cells from healthy terminal ileum surgical tissues were exposed to S . Typhi strain Ty2 (Ty2) at 1:100 MOI. Cells cultured with media only were used as controls (media). After 3 h of incubation, cells were stained using a panel of 33 metal-labeled Abs, and the chromatin modifications were analyzed at the single-cell level by mass cytometry. To visualize the clustering of the 11 cell subsets ( i.e ., B-, CD3+ T-, CD4+ T-, CD8 + T-, NK, TCR-γδ, Mucosal associated invariant (MAIT) and NKT cells, as well as monocytes, macrophages, and epithelial cells), tSNE-defined population distributions and clustering were colored by meta-cluster. ( A ) t-SNE maps of chromatin modifications. Settings to run the t-SNE algorithm were set-up in Cytobank. ( B ) Color-coded key showing the location of epigenetic marks meta-clusters. Data are representative of one out of three experiments with terminal ileum segments from 2 different donors, one replicate each.

Article Snippet: Cells were surface stained with anti-human mAbs to CD3 (clone UCHT1), CD4 (clone SK3), CD8 (clone RPA-T8), CD11b (clone ICRF44), CD16 (clone 3G8), CD19 (clone HIB19), CD38 (clone HIT2), CD45 (clone HI30), CD45RO (clone UCHL), CD56 (clone HCD56), CD57 (clone HCD57), CD69 (clone FN50), CD161 (clone HP-3G10), CD163 (clone GHI/61), CCR7 (clone G043H7), EpCAM (CD326, clone 9C4), HLA-DR (clone L243), TCR γδ (clone 11F2)(Fluidigm, Sunnyvale, CA), CD14 (clone 3C10) (Invitrogen, Carlsbad, CA), and TCR Vα7.2 (clone 3C10) (Biolegend, San Diego, CA).

Techniques: Isolation, Cell Culture, Incubation, Staining, Labeling, Mass Cytometry

Chromatin profiles of the epigenetic changes in TCR-γδ cells induced by S . Typhi. Cells isolated cells from healthy terminal ileum surgical tissues were exposed to S . Typhi strain Ty2 (Ty2) and cultured as described in Fig. . Cells cultured with media only were used as controls (media). FCOM data of the 28 combinations within the acceptability criteria for changes of the chromatin marks are shown. Bars represent the net difference ( S . Typhi-infected minus uninfected cultures). Bar graphs extend from the 25th to 75th percentiles; the line in the middle represents the median of the pooled data. The whiskers delineate the smallest to the largest value. Data are representative of three experiments with terminal ileum segments from 4 different donors, one replicate each. P values < 0.05 were considered significant (red-colored boxes).

Journal: Scientific Reports

Article Title: Salmonella enterica serovar Typhi exposure elicits ex vivo cell-type-specific epigenetic changes in human gut cells

doi: 10.1038/s41598-020-70492-2

Figure Lengend Snippet: Chromatin profiles of the epigenetic changes in TCR-γδ cells induced by S . Typhi. Cells isolated cells from healthy terminal ileum surgical tissues were exposed to S . Typhi strain Ty2 (Ty2) and cultured as described in Fig. . Cells cultured with media only were used as controls (media). FCOM data of the 28 combinations within the acceptability criteria for changes of the chromatin marks are shown. Bars represent the net difference ( S . Typhi-infected minus uninfected cultures). Bar graphs extend from the 25th to 75th percentiles; the line in the middle represents the median of the pooled data. The whiskers delineate the smallest to the largest value. Data are representative of three experiments with terminal ileum segments from 4 different donors, one replicate each. P values < 0.05 were considered significant (red-colored boxes).

Article Snippet: Cells were surface stained with anti-human mAbs to CD3 (clone UCHT1), CD4 (clone SK3), CD8 (clone RPA-T8), CD11b (clone ICRF44), CD16 (clone 3G8), CD19 (clone HIB19), CD38 (clone HIT2), CD45 (clone HI30), CD45RO (clone UCHL), CD56 (clone HCD56), CD57 (clone HCD57), CD69 (clone FN50), CD161 (clone HP-3G10), CD163 (clone GHI/61), CCR7 (clone G043H7), EpCAM (CD326, clone 9C4), HLA-DR (clone L243), TCR γδ (clone 11F2)(Fluidigm, Sunnyvale, CA), CD14 (clone 3C10) (Invitrogen, Carlsbad, CA), and TCR Vα7.2 (clone 3C10) (Biolegend, San Diego, CA).

Techniques: Isolation, Cell Culture, Infection